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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P14211




SWISS-2DPAGE:  P14211


P14211


General information about the entry
View entry in simple text format
Entry nameCALR_MOUSE
Primary accession numberP14211
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 3)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; Flags: Precursor;.
Gene nameName=Calr
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=4.31; Mw=57638
pI=4.29; Mw=54136
pI=4.29; Mw=53630
pI=4.29; Mw=50214

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [1].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=4.41; Mw=62422  [identification data]
pI=4.41; Mw=59639
pI=4.41; Mw=58295
pI=4.41; Mw=55496
pI=4.61; Mw=45248  [identification data]

MAPPING (identification):
SPOTS 2D-0018F3, 2D-0018H*, 2D-0018JF: MATCHING WITH THE MOUSE LIVER MASTER GEL [1]; SPOTS 2D-0018F3, 2D-0018R3: MASS FINGERPRINTING [1].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=4.44; Mw=59607  [identification data]
pI=4.43; Mw=56566  [identification data]
pI=4.43; Mw=52618

MAPPING (identification):
MICROSEQUENCING [1]; SPOTS 2D-00138B, 2D-0013B9: MASS FINGERPRINTING [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.46; Mw=60264  [identification data]

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [1], AND MASS FINGERPRINTING [1].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=4.52; Mw=59451
pI=4.52; Mw=55685
pI=4.52; Mw=52961

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP14211; CALR_MOUSE.
REPRODUCTION-2DPAGEP14211; P14211.
REPRODUCTION-2DPAGEIPI00123639; IPI00123639.
UniProtKB/Swiss-ProtP14211; CALR_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCALR_MOUSE
Primary accession numberP14211
Sequence was last modified on January 1, 1990 (version 1)
Annotations were last modified on October 19, 2011 (version 130)
Name and origin of the protein
DescriptionRecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; Flags: Precursor;
Gene nameName=Calr
Encoded onName=Calr
Keywords3D-structure; Acetylation; Calcium; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Lectin; Metal-binding; Reference proteome; Repeat; Signal; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX14926; CAA33053.1; -; mRNA
EMBLM92988; AAA37569.1; -; mRNA
EMBLAK075605; BAC35852.1; -; mRNA
EMBLBC003453; AAH03453.1; -; mRNA
IPIIPI00123639; -; .
PIRS06763; S06763; .
RefSeqNP_031617.1; NM_007591.3; .
UniGeneMm.1971; -; .
UniGeneMm.467043; -; .
PDB3RG0; X-ray; 2.57 A; A=18-368
PDBsum3RG0; -; .
ProteinModelPortalP14211; -; .
SMRP14211; 16-364; .
IntActP14211; 7; .
STRINGP14211; -; .
PhosphoSiteP14211; -; .
SWISS-2DPAGEP14211; -; .
COMPLUYEAST-2DPAGEP14211; -; .
REPRODUCTION-2DPAGEIPI00123639; -; .
REPRODUCTION-2DPAGEP14211; -; .
PRIDEP14211; -; .
EnsemblENSMUST00000003912; ENSMUSP00000003912; ENSMUSG00000003814; .
GeneID12317; -; .
KEGGmmu:12317; -; .
CTD811; -; .
MGIMGI:88252; Calr; .
eggNOGroNOG10495; -; .
HOGENOMHBG444251; -; .
HOVERGENHBG005407; -; .
InParanoidP14211; -; .
OMADSNIYAY; -; .
OrthoDBEOG41JZCD; -; .
PhylomeDBP14211; -; .
NextBio280884; -; .
ArrayExpressP14211; -; .
BgeeP14211; -; .
CleanExMM_CALR; -; .
GenevestigatorP14211; -; .
GermOnlineENSMUSG00000003814; Mus musculus; .
GOGO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GOGO:0009897; C:external side of plasma membrane; IDA:MGI; .
GOGO:0005615; C:extracellular space; IDA:MGI; .
GOGO:0042824; C:MHC class I peptide loading complex; IMP:BHF-UCL; .
GOGO:0005792; C:microsome; IDA:MGI; .
GOGO:0005844; C:polysome; IDA:BHF-UCL; .
GOGO:0005509; F:calcium ion binding; IDA:UniProtKB; .
GOGO:0003729; F:mRNA binding; IDA:BHF-UCL; .
GOGO:0005529; F:sugar binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0090398; P:cellular senescence; IMP:BHF-UCL; .
GOGO:0030866; P:cortical actin cytoskeleton organization; IDA:MGI; .
GOGO:0002502; P:peptide antigen assembly with MHC class I protein complex; IMP:BHF-UCL; .
GOGO:0045787; P:positive regulation of cell cycle; IMP:BHF-UCL; .
GOGO:0010628; P:positive regulation of gene expression; IMP:UniProtKB; .
GOGO:0050766; P:positive regulation of phagocytosis; IMP:BHF-UCL; .
GOGO:0034504; P:protein localization to nucleus; IMP:UniProtKB; .
GOGO:0050821; P:protein stabilization; IDA:UniProtKB; .
GOGO:0040020; P:regulation of meiosis; IDA:MGI; .
InterProIPR001580; Calret/calnex; .
InterProIPR018124; Calret/calnex_CS; .
InterProIPR009169; Calreticulin; .
InterProIPR008985; ConA-like_lec_gl; .
InterProIPR013320; ConA-like_subgrp; .
Gene3DG3DSA:2.60.120.200; ConA_like_subgrp; 2; .
PANTHERPTHR11073; Calret/calnex; 1; .
PfamPF00262; Calreticulin; 1; .
PIRSFPIRSF002356; Calreticulin; 1; .
PRINTSPR00626; CALRETICULIN; .
SUPFAMSSF49899; ConA_like_lec_gl; 2; .
PROSITEPS00803; CALRETICULIN_1; 1; .
PROSITEPS00804; CALRETICULIN_2; 1; .
PROSITEPS00805; CALRETICULIN_REPEAT; 3; .
PROSITEPS00014; ER_TARGET; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server