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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P69797




SWISS-2DPAGE:  P69797


P69797


General information about the entry
View entry in simple text format
Entry namePTNAB_ECOLI
Primary accession numberP69797
Secondary accession number(s) P08186
integrated into SWISS-2DPAGE on October 1, 1998 (release 8)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=PTS system mannose-specific EIIAB component; AltName: Full=EIIAB-Man; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIII-Man; AltName: Full=PTS system mannose-specific EIIA component; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system mannose-specific EIIB component;.
Gene nameName=manX
Synonyms=gptB, ptsL
OrderedLocusNames=b1817, JW1806
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.17; Mw=26207  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP69797; PTNAB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePTNAB_ECOLI
Primary accession numberP69797
Secondary accession number(s) P08186 Q47350
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 76)
Name and origin of the protein
DescriptionRecName: Full=PTS system mannose-specific EIIAB component; AltName: Full=EIIAB-Man; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIII-Man; AltName: Full=PTS system mannose-specific EIIA component; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system mannose-specific EIIB component;
Gene nameName=manX
Synonyms=gptB, ptsL
OrderedLocusNames=b1817, JW1806
Encoded onName=manX; Synonyms=gptB, ptsL; OrderedLocusNames=b1817, JW1806
Keywords3D-structure; Acetylation; Cell inner membrane; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM36404; AAA24110.1; -; Genomic_DNA
EMBLJ02699; AAA24443.1; -; Genomic_DNA
EMBLU00096; AAC74887.1; -; Genomic_DNA
EMBLAP009048; BAA15624.1; -; Genomic_DNA
PIRA30286; WQECM3; .
RefSeqNP_416331.1; NC_000913.2; .
PDB1PDO; X-ray; 1.70 A; A=2-133
PDB1VRC; NMR; -; A/B=2-132
PDB1VSQ; NMR; -; A/B=2-134
C=159-323PDB; 2JZH; NMR; -
A=154-323PDB; 2JZN; NMR; -
A/B=2-134C=159-323; PDB; 2JZO; NMR
-A/B=2-134; D=159-323; PDBsum; 1PDO
PDBsum1VRC; -; .
PDBsum1VSQ; -; .
PDBsum2JZH; -; .
PDBsum2JZN; -; .
PDBsum2JZO; -; .
ProteinModelPortalP69797; -; .
SMRP69797; 2-130; 159-323; .
DIPDIP-35846N; -; .
IntActP69797; 12; .
MINTMINT-1228428; -; .
TCDB4.A.6.1.1; PTS mannose-fructose-sorbose (Man) family; .
PhosSiteP69797; -; .
SWISS-2DPAGEP69797; -; .
PRIDEP69797; -; .
EnsemblBacteriaEBESCT00000001866; EBESCP00000001866; EBESCG00000001533; .
EnsemblBacteriaEBESCT00000018240; EBESCP00000017531; EBESCG00000017295; .
GeneID946334; -; .
GenomeReviewsAP009048_GR; JW1806; .
GenomeReviewsU00096_GR; b1817; .
KEGGecj:JW1806; -; .
KEGGeco:b1817; -; .
EchoBASEEB0562; -; .
EcoGeneEG10567; manX; .
eggNOGCOG3444; -; .
GeneTreeEBGT00050000009724; -; .
HOGENOMHBG296634; -; .
OMATTEMLIG; -; .
ProtClustDBPRK15088; -; .
BioCycEcoCyc:MANX-MONOMER; -; .
GenevestigatorP69797; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0016021; C:integral to membrane; IEA:InterPro; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0016301; F:kinase activity; IEA:UniProtKB-KW; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC; .
GOGO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW; .
InterProIPR004701; PTS_EIIA_fruc; .
InterProIPR013789; PTS_EIIA_fruc_subgr; .
InterProIPR004720; PTS_IIB_sorbose-sp; .
InterProIPR018455; PTS_IIB_sorbose-sp_subgr; .
Gene3DG3DSA:3.40.50.510; PTS_EIIA_fruc; 1; .
Gene3DG3DSA:3.40.35.10; PTS_IIB_sorb; 1; .
PfamPF03610; EIIA-man; 1; .
PfamPF03830; PTSIIB_sorb; 1; .
SUPFAMSSF53062; PTS_EIIA_fruc; 1; .
SUPFAMSSF52728; PTS_IIB_sorb; 1; .
TIGRFAMsTIGR00824; EIIA-man; 1; .
TIGRFAMsTIGR00854; Pts-sorbose; 1; .
PROSITEPS51096; PTS_EIIA_TYPE_4; 1; .
PROSITEPS51101; PTS_EIIB_TYPE_4; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server