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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: AAT_ECOLI




SWISS-2DPAGE:  AAT_ECOLI


AAT_ECOLI


General information about the entry
View entry in simple text format
Entry nameAAT_ECOLI
Primary accession numberP00509
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Aspartate aminotransferase; Short=AspAT; EC=2.6.1.1; AltName: Full=Transaminase A;.
Gene nameName=aspC
OrderedLocusNames=b0928, JW0911
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=81069671; PubMed=7002901; [NCBI, ExPASy, EBI, Israel, Japan]
Phillips T.A., Bloch P.L., Neidhardt F.C.
''''''Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins'';'';''
J. Bacteriol. 144(1):1024-1033(1980)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.40; Mw=42043
pI=5.52; Mw=41960

MAPPING (identification):
TAGGING ANALYSIS AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND PHILLIPS [3].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.53; Mw=50441  [identification data]
pI=5.56; Mw=49421  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEF039.6; 6TH EDITION.
ECO2DBASEF039.7; 6TH EDITION.
UniProtKB/Swiss-ProtP00509; AAT_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameAAT_ECOLI
Primary accession numberP00509
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 131)
Name and origin of the protein
DescriptionRecName: Full=Aspartate aminotransferase; Short=AspAT; EC=2.6.1.1; AltName: Full=Transaminase A;
Gene nameName=aspC
OrderedLocusNames=b0928, JW0911
Encoded onName=aspC; OrderedLocusNames=b0928, JW0911
Keywords3D-structure; Aminotransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX03629; CAA27279.1; -; Genomic_DNA
EMBLX05904; CAA29333.1; -; Genomic_DNA
EMBLU00096; AAC74014.1; -; Genomic_DNA
EMBLAP009048; BAA35674.1; -; Genomic_DNA
PIRA00598; XNECD; .
RefSeqNP_415448.1; NC_000913.2; .
PDB1AAM; X-ray; 2.80 A; A=1-396
PDB1AAW; X-ray; 2.40 A; A=1-396
PDB1AHE; X-ray; 2.30 A; A/B=1-396
PDB1AHF; X-ray; 2.30 A; A/B=1-396
PDB1AHG; X-ray; 2.50 A; A/B=1-396
PDB1AHX; X-ray; 2.00 A; A/B=1-396
PDB1AHY; X-ray; 2.30 A; A/B=1-396
PDB1AIA; X-ray; 2.20 A; A/B=1-396
PDB1AIB; X-ray; 2.80 A; A/B=1-396
PDB1AIC; X-ray; 2.40 A; A/B=1-396
PDB1AMQ; X-ray; 2.20 A; A=1-396
PDB1AMR; X-ray; 2.10 A; A=1-396
PDB1AMS; X-ray; 2.70 A; A=1-396
PDB1ARG; X-ray; 2.20 A; A/B=1-396
PDB1ARH; X-ray; 2.30 A; A/B=1-396
PDB1ARI; X-ray; 2.30 A; A/B=1-396
PDB1ARS; X-ray; 1.80 A; A=1-396
PDB1ART; X-ray; 1.80 A; A=1-396
PDB1ASA; X-ray; 2.40 A; A=1-396
PDB1ASB; X-ray; 2.60 A; A=1-396
PDB1ASC; X-ray; 2.40 A; A=1-396
PDB1ASD; X-ray; 2.20 A; A=1-396
PDB1ASE; X-ray; 2.50 A; A=1-396
PDB1ASF; X-ray; 2.80 A; A=1-396
PDB1ASG; X-ray; 2.80 A; A=1-396
PDB1ASL; X-ray; 2.60 A; A/B=1-396
PDB1ASM; X-ray; 2.35 A; A/B=1-396
PDB1ASN; X-ray; 2.50 A; A/B=1-396
PDB1B4X; X-ray; 2.45 A; A=1-396
PDB1BQA; X-ray; 2.10 A; A/B=1-396
PDB1BQD; X-ray; 2.10 A; A/B=1-396
PDB1C9C; X-ray; 2.40 A; A=1-396
PDB1CQ6; X-ray; 2.70 A; A=1-396
PDB1CQ7; X-ray; 2.40 A; A=1-396
PDB1CQ8; X-ray; 2.40 A; A=1-396
PDB1CZC; X-ray; 2.50 A; A=1-396
PDB1CZE; X-ray; 2.40 A; A=1-396
PDB1G4V; X-ray; 2.00 A; A=1-396
PDB1G4X; X-ray; 2.20 A; A=1-396
PDB1G7W; X-ray; 2.20 A; A=1-396
PDB1G7X; X-ray; 2.20 A; A=1-396
PDB1IX6; X-ray; 2.20 A; A=1-396
PDB1IX7; X-ray; 2.20 A; A=1-396
PDB1IX8; X-ray; 2.20 A; A=1-396
PDB1QIR; X-ray; 2.20 A; A=1-396
PDB1QIS; X-ray; 1.90 A; A=1-396
PDB1QIT; X-ray; 1.90 A; A=1-396
PDB1SPA; X-ray; 2.00 A; A=1-396
PDB1TOE; X-ray; 2.00 A; A=1-388
PDB1TOG; X-ray; 2.31 A; A/B=1-388
PDB1TOI; X-ray; 1.90 A; A=1-388
PDB1TOJ; X-ray; 1.90 A; A=1-388
PDB1TOK; X-ray; 1.85 A; A/B=1-388
PDB1X28; X-ray; 2.40 A; A/B=1-396
PDB1X29; X-ray; 2.20 A; A/B=1-396
PDB1X2A; X-ray; 2.20 A; A/B=1-396
PDB1YOO; X-ray; 2.40 A; A=1-396
PDB2AAT; X-ray; 2.80 A; A=1-396
PDB2D5Y; X-ray; 1.98 A; A=1-396
PDB2D61; X-ray; 2.01 A; A=1-396
PDB2D63; X-ray; 2.05 A; A=1-396
PDB2D64; X-ray; 2.05 A; A=1-396
PDB2D65; X-ray; 2.30 A; A=1-396
PDB2D66; X-ray; 2.18 A; A=1-396
PDB2D7Y; X-ray; 2.66 A; A=1-396
PDB2D7Z; X-ray; 2.65 A; A=1-396
PDB2Q7W; X-ray; 1.40 A; A=1-396
PDB2QA3; X-ray; 1.75 A; A=1-396
PDB2QB2; X-ray; 1.70 A; A=1-396
PDB2QB3; X-ray; 1.45 A; A=1-396
PDB2QBT; X-ray; 1.75 A; A=1-396
PDB3AAT; X-ray; 2.80 A; A=1-396
PDB3QN6; X-ray; 1.79 A; A=1-396
PDB3QPG; X-ray; 1.79 A; A=1-396
PDB5EAA; X-ray; 2.40 A; A=1-396
PDBsum1AAM; -; .
PDBsum1AAW; -; .
PDBsum1AHE; -; .
PDBsum1AHF; -; .
PDBsum1AHG; -; .
PDBsum1AHX; -; .
PDBsum1AHY; -; .
PDBsum1AIA; -; .
PDBsum1AIB; -; .
PDBsum1AIC; -; .
PDBsum1AMQ; -; .
PDBsum1AMR; -; .
PDBsum1AMS; -; .
PDBsum1ARG; -; .
PDBsum1ARH; -; .
PDBsum1ARI; -; .
PDBsum1ARS; -; .
PDBsum1ART; -; .
PDBsum1ASA; -; .
PDBsum1ASB; -; .
PDBsum1ASC; -; .
PDBsum1ASD; -; .
PDBsum1ASE; -; .
PDBsum1ASF; -; .
PDBsum1ASG; -; .
PDBsum1ASL; -; .
PDBsum1ASM; -; .
PDBsum1ASN; -; .
PDBsum1B4X; -; .
PDBsum1BQA; -; .
PDBsum1BQD; -; .
PDBsum1C9C; -; .
PDBsum1CQ6; -; .
PDBsum1CQ7; -; .
PDBsum1CQ8; -; .
PDBsum1CZC; -; .
PDBsum1CZE; -; .
PDBsum1G4V; -; .
PDBsum1G4X; -; .
PDBsum1G7W; -; .
PDBsum1G7X; -; .
PDBsum1IX6; -; .
PDBsum1IX7; -; .
PDBsum1IX8; -; .
PDBsum1QIR; -; .
PDBsum1QIS; -; .
PDBsum1QIT; -; .
PDBsum1SPA; -; .
PDBsum1TOE; -; .
PDBsum1TOG; -; .
PDBsum1TOI; -; .
PDBsum1TOJ; -; .
PDBsum1TOK; -; .
PDBsum1X28; -; .
PDBsum1X29; -; .
PDBsum1X2A; -; .
PDBsum1YOO; -; .
PDBsum2AAT; -; .
PDBsum2D5Y; -; .
PDBsum2D61; -; .
PDBsum2D63; -; .
PDBsum2D64; -; .
PDBsum2D65; -; .
PDBsum2D66; -; .
PDBsum2D7Y; -; .
PDBsum2D7Z; -; .
PDBsum2Q7W; -; .
PDBsum2QA3; -; .
PDBsum2QB2; -; .
PDBsum2QB3; -; .
PDBsum2QBT; -; .
PDBsum3AAT; -; .
PDBsum3QN6; -; .
PDBsum3QPG; -; .
PDBsum5EAA; -; .
ProteinModelPortalP00509; -; .
SMRP00509; 1-396; .
DIPDIP-9181N; -; .
IntActP00509; 4; .
SWISS-2DPAGEP00509; -; .
ECO2DBASEF039.6; 6TH EDITION; .
ECO2DBASEF039.7; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004900; EBESCP00000004900; EBESCG00000004001; .
EnsemblBacteriaEBESCT00000016426; EBESCP00000015717; EBESCG00000015486; .
GeneID945553; -; .
GenomeReviewsAP009048_GR; JW0911; .
GenomeReviewsU00096_GR; b0928; .
KEGGecj:JW0911; -; .
KEGGeco:b0928; -; .
EchoBASEEB0094; -; .
EcoGeneEG10096; aspC; .
eggNOGCOG1448; -; .
GeneTreeEBGT00050000010085; -; .
HOGENOMHBG446828; -; .
OMAPNHNAIA; -; .
ProtClustDBPRK09257; -; .
BioCycEcoCyc:ASPAMINOTRANS-MONOMER; -; .
BioCycMetaCyc:ASPAMINOTRANS-MONOMER; -; .
BRENDA2.6.1.1; 2026; .
GenevestigatorP00509; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016212; F:kynurenine-oxoglutarate transaminase activity; IDA:EcoliWiki; .
GOGO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; IDA:EcoliWiki; .
GOGO:0004838; F:L-tyrosine:2-oxoglutarate aminotransferase activity; IMP:EcoliWiki; .
GOGO:0042803; F:protein homodimerization activity; IDA:EcoCyc; .
GOGO:0030170; F:pyridoxal phosphate binding; IDA:EcoliWiki; .
GOGO:0006532; P:aspartate biosynthetic process; IMP:EcoliWiki; .
GOGO:0033585; P:L-phenylalanine biosynthetic process from chorismate via phenylpyruvate; IMP:EcoCyc; .
InterProIPR004839; Aminotransferase_I/II; .
InterProIPR000796; Asp_trans; .
InterProIPR004838; NHTrfase_class1_PyrdxlP-BS; .
InterProIPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterProIPR015421; PyrdxlP-dep_Trfase_major_sub1; .
Gene3DG3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
PANTHERPTHR11879; Asp_trans; 1; .
PfamPF00155; Aminotran_1_2; 1; .
PRINTSPR00799; TRANSAMINASE; .
SUPFAMSSF53383; PyrdxlP-dep_Trfase_major; 1; .
PROSITEPS00105; AA_TRANSFER_CLASS_1; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server