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Sample Preparation and Post-separation Analysis

ACADS_HUMAN:

ID   ACADS_HUMAN; STANDARD; 2DG.
AC   P16219;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   01-DEC-2000, 2D annotation version 3.
DT   09-NOV-2011, general annotation version 12.
DE   RecName: Full=Short-chain specific acyl-CoA dehydrogenase, mitochondrial;
DE   Short=SCAD; EC=1.3.8.1; AltName: Full=Butyryl-CoA dehydrogenase; Flags: Precursor;.
GN   Name=ACADS;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, HEPG2_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN.
IM   DLD1_HUMAN, HEPG2_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [4]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001E9S=6.32/37524;
2D   -!-   MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [4].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00034Y=6.50/38000;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000NB3=6.41/37376;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000106=6.38/38095;
2D   -!-   PATHOLOGICAL LEVEL: DECREASED DURING ACUTE ACIDOSIS AND
2D         LIPID-STORAGE MYOPATHY.
2D   -!-   MAPPING: MICROSEQUENCING [1].
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DR   DOSAC-COBS 2D-PAGE; P16219; P16219.
DR   DOSAC-COBS-2DPAGE; P16219; ACADS_HUMAN.
DR   UCD-2DPAGE; P16219; ACADS_HUMAN.
DR   UniProtKB/Swiss-Prot; P16219; ACADS_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)	HepG2	Kidney
	Liver

SWISS-2DPAGE (search AC)