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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: ACKA_ECOLI |
SWISS-2DPAGE: ACKA_ECOLI
ACKA_ECOLI:
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ID ACKA_ECOLI; STANDARD; 2DG.
AC P0A6A3; P15046;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Acetate kinase; EC=2.7.2.1; AltName: Full=Acetokinase;.
GN Name=ackA; Synonyms=ack; OrderedLocusNames=b2296, JW2293;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [4]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KXK=5.84/44088;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LH3=5.72/31846;
2D -!- PEPTIDE MASSES: SPOT 2D-001LH3: 1102.49; 1330.642; 1346.638;
2D 1370.713; 1835.942; 1838.925; 1866.879; 2022.965; 2465.251; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WMA=5.79/41417;
2D -!- PEPTIDE MASSES: SPOT 2D-001WMA: 1102.42; 1370.68; 1389.74; 1636.81;
2D 1642.87; 1737.91; 1866.91; 2314.24; 2465.31; 2921.45; 4011.37; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WMA: (K)LVLVLNCGSSSLK(F),5-17;
2D (K)FAIIDAVNGEEYLSGLAECFHLPEAR(I),18-43; (K)YTSSVVIDESVIQGIK(D),99-114;
2D (K)ESGLLGLTEVTSDCR(Y),273-287; (R)YVEDNYATK(E),288-296; (K)EGTRPAVVIPTNEELVIAQDASR(L),375-397.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [4].
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DR ECO2DBASE; G041.3; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A6A3; ACKA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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