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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ACTB_MOUSE




SWISS-2DPAGE:  ACTB_MOUSE


ACTB_MOUSE


General information about the entry
View entry in simple text format
Entry nameACTB_MOUSE
Primary accession numberP99041
Secondary accession number(s) P99020 P99021 P99022
integrated into SWISS-2DPAGE on April 1, 2000 (release 12)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;.
Gene nameName=Actb
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
MEDLINE=93162044; PubMed=1286668; [NCBI, ExPASy, EBI, Israel, Japan]
Giometti C.S., Taylor J., Tollaksen S.L.
''''''Mouse liver protein database: a catalog of proteins detected by two- dimensional gel electrophoresis'';'';''
Electrophoresis 13(1):970-991(1992)
[3]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=5.10; Mw=40780
pI=5.17; Mw=40780
pI=5.21; Mw=40780
pI=5.25; Mw=40780
pI=5.27; Mw=40880
pI=5.02; Mw=40780
pI=5.29; Mw=40780
pI=4.96; Mw=40730
pI=5.28; Mw=40730

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [3].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=5.12; Mw=48613  [identification data]
pI=5.16; Mw=48747  [identification data]
pI=5.17; Mw=44382  [identification data]
pI=5.21; Mw=44505  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=5.15; Mw=44157  [identification data]
pI=5.26; Mw=41907
pI=5.27; Mw=42117

MAPPING (identification):
SPOT 2D-0013LF: Peptide mass fingerprinting [3]; SPOTS 2D-0013N*: MATCHING [3] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1], AND WITH GIOMETTI MOUSE LIVER 2-DE MAP [2].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=5.20; Mw=50527
pI=5.19; Mw=47815
pI=5.20; Mw=44017  [identification data]
pI=5.13; Mw=43175  [identification data]
pI=5.06; Mw=43414  [identification data]
pI=5.27; Mw=42937  [identification data]
pI=5.09; Mw=41351  [identification data]

MAPPING (identification):
SPOTS 2D-0016*, 2D-0017*: MATCHING [3] WITH THE MOUSE CARDIAC 2-DE GEL [3]; SPOTS 2D-0016ZF, 2D-0017*: MASS FINGERPRINTING [3].



NUCLEI_LIVER_MOUSE {Soluble nuclear proteins and matrix from liver tissue}
Mus musculus (Mouse)
Tissue: Liver
NUCLEI_LIVER_MOUSE
  map experimental info
  protein estimated location
 
NUCLEI_LIVER_MOUSE

MAP LOCATIONS:
pI=5.24; Mw=41605  [identification data]
pI=5.14; Mw=41718  [identification data]
pI=5.05; Mw=41946  [identification data]
pI=5.19; Mw=41504  [identification data]
pI=4.57; Mw=27449  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=5.27; Mw=42000  [identification data]
pI=5.23; Mw=42000
pI=5.26; Mw=41845  [identification data]
pI=5.18; Mw=42132

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [3]; SPOTS 1BBI, 1BBV: Peptide mass fingerprinting [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP60710; ACTB_MOUSE.
REPRODUCTION-2DPAGEIPI00110850; IPI00110850.
REPRODUCTION-2DPAGEP60710; P60710.
UCD-2DPAGEP60710; ACTB_MOUSE.
UniProtKB/Swiss-ProtP60710; ACTB_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameACTB_MOUSE
Primary accession numberP60710
Secondary accession number(s) P02570 P70514 P99021 Q11211 Q3TI89 Q3TVP6 Q64316 Q6ZWM3
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 85)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;
Gene nameName=Actb
Encoded onName=Actb
KeywordsAcetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methylation; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX03672; CAA27307.1; -; mRNA
EMBLAK088691; BAC40507.1; -; mRNA
EMBLAK145191; BAE26283.1; -; mRNA
EMBLAK145196; BAE26288.1; -; mRNA
EMBLAK145308; BAE26359.1; -; mRNA
EMBLAK150711; BAE29789.1; -; mRNA
EMBLAK150879; BAE29928.1; -; mRNA
EMBLAK151010; BAE30031.1; -; mRNA
EMBLAK151136; BAE30144.1; -; mRNA
EMBLAK151145; BAE30152.1; -; mRNA
EMBLAK151159; BAE30164.1; -; mRNA
EMBLAK151166; BAE30169.1; -; mRNA
EMBLAK151190; BAE30187.1; -; mRNA
EMBLAK151202; BAE30199.1; -; mRNA
EMBLAK151226; BAE30218.1; -; mRNA
EMBLAK151277; BAE30264.1; -; mRNA
EMBLAK151350; BAE30326.1; -; mRNA
EMBLAK151398; BAE30366.1; -; mRNA
EMBLAK151995; BAE30859.1; -; mRNA
EMBLAK151999; BAE30863.1; -; mRNA
EMBLAK152615; BAE31359.1; -; mRNA
EMBLAK152651; BAE31388.1; -; mRNA
EMBLAK152844; BAE31537.1; -; mRNA
EMBLAK159759; BAE35350.1; -; mRNA
EMBLAK159834; BAE35412.1; -; mRNA
EMBLAK160029; BAE35572.1; -; mRNA
EMBLAK166349; BAE38723.1; -; mRNA
EMBLAK166498; BAE38810.1; -; mRNA
EMBLAK167117; BAE39265.1; -; mRNA
EMBLAK167960; BAE39957.1; -; mRNA
EMBLX03765; CAA27396.1; -; mRNA
EMBLM12481; AAA37144.1; -; mRNA
IPIIPI00110850; -; .
PIRA39104; ATMSB; .
RefSeqNP_031419.1; NM_007393.3; .
UniGeneMm.328431; -; .
UniGeneMm.391967; -; .
ProteinModelPortalP60710; -; .
SMRP60710; 2-375; .
IntActP60710; 17; .
MINTMINT-1202772; -; .
STRINGP60710; -; .
PhosphoSiteP60710; -; .
SWISS-2DPAGEP99041; -; .
COMPLUYEAST-2DPAGEP60710; -; .
REPRODUCTION-2DPAGEP60710; -; .
UCD-2DPAGEP60710; -; .
PRIDEP60710; -; .
EnsemblENSMUST00000100497; ENSMUSP00000098066; ENSMUSG00000029580; .
GeneID11461; -; .
KEGGmmu:11461; -; .
CTD60; -; .
MGIMGI:87904; Actb; .
eggNOGroNOG11419; -; .
HOVERGENHBG003771; -; .
InParanoidP60710; -; .
OMAIKNLMER; -; .
OrthoDBEOG41JZC9; -; .
PhylomeDBP60710; -; .
NextBio278784; -; .
ArrayExpressP60710; -; .
BgeeP60710; -; .
CleanExMM_ACTB; -; .
GenevestigatorP60710; -; .
GermOnlineENSMUSG00000029580; Mus musculus; .
GOGO:0030863; C:cortical cytoskeleton; IDA:MGI; .
GOGO:0005829; C:cytosol; IDA:MGI; .
GOGO:0005625; C:soluble fraction; IDA:MGI; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
InterProIPR004000; Actin-like; .
InterProIPR020902; Actin/actin-like_CS; .
InterProIPR004001; Actin_CS; .
PANTHERPTHR11937; Actin_like; 1; .
PfamPF00022; Actin; 1; .
PRINTSPR00190; ACTIN; .
SMARTSM00268; ACTIN; 1; .
PROSITEPS00406; ACTINS_1; 1; .
PROSITEPS00432; ACTINS_2; 1; .
PROSITEPS01132; ACTINS_ACT_LIKE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server