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Sample Preparation and Post-separation Analysis

AHPF_ECOLI:

ID   AHPF_ECOLI; STANDARD; 2DG.
AC   P35340;
DT   01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 8.
DE   RecName: Full=Alkyl hydroperoxide reductase subunit F; EC=1.8.1.-;
DE   AltName: Full=Alkyl hydroperoxide reductase F52A protein;.
GN   Name=ahpF; OrderedLocusNames=b0606, JW0599;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LA1=5.20/41503; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LA1: 929.545; 930.566; 1186.742;
2D         1330.817; 1404.86; 1713.053; 1774.975; 2006.097; 2016.168; 2174.378;
2D         2208.271; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WHP=5.44/53485;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WHP: (K)LTKPVELIATLDDSAK(S),17-32;
2D         (K)ELLAEIAELSDK(V),38-49; (K)EDNSLPVR(K),54-61; (K)EAQSLLEQIR(H),104-113;
2D         (R)FGGQILDTVDIENYISVPK(T),245-263; (R)SIIVATGAK(W),317-325;
2D         (K)ADQVLQDK(L),392-399; (K)QIIIATGEGAK(A),496-506; (K)ASLSAFDYLIR(T),507-517.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [2].
2D   -!-   MAPPING: Tandem mass spectrometry [2].
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DR   UniProtKB/Swiss-Prot; P35340; AHPF_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli(5-6)	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)