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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ALF_ECOLI




SWISS-2DPAGE: ALF_ECOLI



ALF_ECOLI:

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ID   ALF_ECOLI; STANDARD; 2DG.
AC   P0AB71; P11604;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Fructose-bisphosphate aldolase class 2; Short=FBP aldolase;
DE   Short=FBPA; EC=4.1.2.13; AltName: Full=Fructose-1,6-bisphosphate
DE   aldolase; AltName: Full=Fructose-bisphosphate aldolase class II;.
GN   Name=fbaA; Synonyms=fba, fda; OrderedLocusNames=b2925, JW2892;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L0H=5.55/40732;
2D   -!-   PI/MW: SPOT 2D-000L1R=5.43/39855;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000L1R: B=10.9, Z=10.5, S=7.2, H=3,
2D         G=10.5, T=5.3, A=9.4, P=4.3, Y=3.6, R=3.2, V=7.4, M=1.7, I=5.3, L=8.6,
2D         F=4.3, K=4.8;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (SKIF) [1].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001L5L=5.56/50220;
2D   -!-   PI/MW: SPOT 2D-001L6U=5.56/49421;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L5L: 1320.801; 1502.988; 1878.257;
2D         2591.649; 2719.736; 2871.916; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L6U: 934.591; 950.583; 953.573;
2D         1320.797; 1502.947; 1878.221; 2591.534; 2719.66; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WMY=5.49/39104;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WMY: 955.51; 1502.78; 1762.98; 1878.01; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [3].
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DR   2DBase-Ecoli; P0AB71; ALF_ECOLI.
DR   UniProtKB/Swiss-Prot; P0AB71; ALF_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server