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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ALKH_ECOLI




SWISS-2DPAGE:  ALKH_ECOLI


ALKH_ECOLI


General information about the entry
View entry in simple text format
Entry nameALKH_ECOLI
Primary accession numberP0A955
Secondary accession number(s) P10177
integrated into SWISS-2DPAGE on October 31, 1999 (release 11)
2D Annotations were last modified onDecember 30, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=KHG/KDPG aldolase; Includes: RecName: Full=4-hydroxy-2-oxoglutarate aldolase; EC=4.1.3.16; AltName: Full=2-keto-4-hydroxyglutarate aldolase; Short=KHG-aldolase; Includes: RecName: Full=2-dehydro-3-deoxy-phosphogluconate aldolase; EC=4.1.2.14; AltName: Full=2-keto-3-deoxy-6-phosphogluconate aldolase; Short=KDPG-aldolase; AltName: Full=Phospho-2-dehydro-3-deoxygluconate aldolase; AltName: Full=Phospho-2-keto-3-deoxygluconate aldolase;.
Gene nameName=eda
Synonyms=hga, kdgA
OrderedLocusNames=b1850, JW1839
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.43; Mw=27937  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=4.93; Mw=23247  [identification data]

EXPRESSION:
increase after benzoic acid treatment [2].

MAPPING (identification):
Peptide mass fingerprinting [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEF022.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A955; ALKH_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameALKH_ECOLI
Primary accession numberP0A955
Secondary accession number(s) P10177
Sequence was last modified on July 19, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 57)
Name and origin of the protein
DescriptionRecName: Full=KHG/KDPG aldolase; Includes: RecName: Full=4-hydroxy-2-oxoglutarate aldolase; EC=4.1.3.16; AltName: Full=2-keto-4-hydroxyglutarate aldolase; Short=KHG-aldolase; Includes: RecName: Full=2-dehydro-3-deoxy-phosphogluconate aldolase; EC=4.1.2.14; AltName: Full=2-keto-3-deoxy-6-phosphogluconate aldolase; Short=KDPG-aldolase; AltName: Full=Phospho-2-dehydro-3-deoxygluconate aldolase; AltName: Full=Phospho-2-keto-3-deoxygluconate aldolase;
Gene nameName=eda
Synonyms=hga, kdgA
OrderedLocusNames=b1850, JW1839
Encoded onName=eda; Synonyms=hga, kdgA; OrderedLocusNames=b1850, JW1839
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Multifunctional enzyme; Reference proteome; Schiff base.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX68871; CAA48732.1; -; Genomic_DNA
EMBLM87458; AAA23723.1; -; Genomic_DNA
EMBLL20897; AAA23862.1; -; Genomic_DNA
EMBLX63694; CAA45222.1; -; Genomic_DNA
EMBLU00096; AAC74920.1; -; Genomic_DNA
EMBLAP009048; BAA15658.1; -; Genomic_DNA
PIRB42986; ADECOG; .
RefSeqNP_416364.1; NC_000913.2; .
PDB1EUA; X-ray; 1.95 A; A/B/C=1-213
PDB1EUN; X-ray; 2.00 A; A/B/C=1-213
PDB1FQ0; X-ray; 2.10 A; A/B/C=1-213
PDB1FWR; X-ray; 2.70 A; A/B/C=1-213
PDB1WAU; X-ray; 2.80 A; A=1-213
PDB1WBH; X-ray; 1.55 A; A/B/C=1-213
PDB2C0A; X-ray; 1.55 A; A/B/C=1-213
PDBsum1EUA; -; .
PDBsum1EUN; -; .
PDBsum1FQ0; -; .
PDBsum1FWR; -; .
PDBsum1WAU; -; .
PDBsum1WBH; -; .
PDBsum2C0A; -; .
ProteinModelPortalP0A955; -; .
SMRP0A955; 1-213; .
DIPDIP-36196N; -; .
IntActP0A955; 8; .
SWISS-2DPAGEP0A955; -; .
ECO2DBASEF022.5; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001939; EBESCP00000001939; EBESCG00000001592; .
EnsemblBacteriaEBESCT00000001940; EBESCP00000001940; EBESCG00000001592; .
EnsemblBacteriaEBESCT00000001941; EBESCP00000001941; EBESCG00000001592; .
EnsemblBacteriaEBESCT00000001942; EBESCP00000001942; EBESCG00000001592; .
EnsemblBacteriaEBESCT00000018400; EBESCP00000017691; EBESCG00000017454; .
GeneID946367; -; .
GenomeReviewsAP009048_GR; JW1839; .
GenomeReviewsU00096_GR; b1850; .
KEGGecj:JW1839; -; .
KEGGeco:b1850; -; .
EchoBASEEB0252; -; .
EcoGeneEG10256; eda; .
eggNOGCOG0800; -; .
GeneTreeEBGT00050000010303; -; .
HOGENOMHBG670118; -; .
OMAMKFFPAE; -; .
ProtClustDBPRK05718; -; .
BioCycEcoCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER; -; .
BioCycMetaCyc:KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER; -; .
GenevestigatorP0A955; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IMP:EcoliWiki; .
GOGO:0008700; F:4-hydroxy-2-oxoglutarate aldolase activity; IEA:EC; .
InterProIPR000887; Aldlse_KDPG_KHG; .
InterProIPR013785; Aldolase_TIM; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PfamPF01081; Aldolase; 1; .
TIGRFAMsTIGR01182; Eda; 1; .
PROSITEPS00159; ALDOLASE_KDPG_KHG_1; 1; .
PROSITEPS00160; ALDOLASE_KDPG_KHG_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server