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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: APOE_HUMAN




SWISS-2DPAGE:  APOE_HUMAN


APOE_HUMAN


General information about the entry
View entry in simple text format
Entry nameAPOE_HUMAN
Primary accession numberP02649
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;.
Gene nameName=APOE
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Anderson N.G.
''''''High resolution two-dimensional electrophoresis of human plasma proteins'';'';''
Proc. Natl. Acad. Sci. U.S.A. 74(1):5421-5425(1977)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=5.07; Mw=34489
pI=5.23; Mw=33314  [identification data]
pI=5.34; Mw=32904
pI=5.47; Mw=31940

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=5.24; Mw=35320
pI=5.35; Mw=34966
pI=5.49; Mw=34200

NORMAL LEVEL:
30-50 MG/L.

PATHOLOGICAL LEVEL:
APOE ABNORMALITIES IN TYPE III AND IV HYPERLIPAEMIA.

POSITIONAL VARIANTS:
APOE 2-2, 2-3, 2-4, 3-3, 3-4 AND 4-4.

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP02649; P02649.
DOSAC-COBS-2DPAGEP02649; APOE_HUMAN.
UniProtKB/Swiss-ProtP02649; APOE_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameAPOE_HUMAN
Primary accession numberP02649
Secondary accession number(s) B2RC15 C0JYY5 Q9P2S4
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 160)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;
Gene nameName=APOE
Encoded onName=APOE
Keywords3D-structure; Alzheimer disease; Amyloidosis; Chylomicron; Complete proteome; Direct protein sequencing; Disease mutation; Glycation; Glycoprotein; HDL; Heparin-binding; Hyperlipidemia; Lipid transport; Neurodegeneration; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Secreted; Signal; Transport; VLDL.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM12529; AAB59518.1; -; mRNA
EMBLK00396; AAB59546.1; -; mRNA
EMBLM10065; AAB59397.1; -; Genomic_DNA
EMBLAF050154; AAD02505.1; -; Genomic_DNA
EMBLAF261279; AAG27089.1; -; Genomic_DNA
EMBLAK314898; BAG37412.1; -; mRNA
EMBLFJ525876; ACN81314.1; -; Genomic_DNA
EMBLBC003557; AAH03557.1; -; mRNA
EMBLAB035149; BAA96080.1; -; Genomic_DNA
IPIIPI00021842; -; .
PIRA92478; LPHUE; .
RefSeqNP_000032.1; NM_000041.2; .
UniGeneHs.654439; -; .
PDB1B68; X-ray; 2.00 A; A=19-209
PDB1BZ4; X-ray; 1.85 A; A=40-183
PDB1EA8; X-ray; 1.95 A; A=19-209
PDB1GS9; X-ray; 1.70 A; A=19-183
PDB1H7I; X-ray; 1.90 A; A=19-209
PDB1LE2; X-ray; 3.00 A; A=41-184
PDB1LE4; X-ray; 2.50 A; A=41-184
PDB1LPE; X-ray; 2.25 A; A=41-184
PDB1NFN; X-ray; 1.80 A; A=19-209
PDB1NFO; X-ray; 2.00 A; A=19-209
PDB1OEF; NMR; -; A=281-304
PDB1OEG; NMR; -; A=285-307
PDB1OR2; X-ray; 2.50 A; A=19-183
PDB1OR3; X-ray; 1.73 A; A=19-183
PDB2KC3; NMR; -; A=19-201
PDB2KNY; NMR; -; A=147-167
PDB2L7B; NMR; -; A=19-317
PDBsum1B68; -; .
PDBsum1BZ4; -; .
PDBsum1EA8; -; .
PDBsum1GS9; -; .
PDBsum1H7I; -; .
PDBsum1LE2; -; .
PDBsum1LE4; -; .
PDBsum1LPE; -; .
PDBsum1NFN; -; .
PDBsum1NFO; -; .
PDBsum1OEF; -; .
PDBsum1OEG; -; .
PDBsum1OR2; -; .
PDBsum1OR3; -; .
PDBsum2KC3; -; .
PDBsum2KNY; -; .
PDBsum2L7B; -; .
ProteinModelPortalP02649; -; .
SMRP02649; 19-304; .
DisProtDP00355; -; .
DIPDIP-1120N; -; .
IntActP02649; 15; .
MINTMINT-4999641; -; .
STRINGP02649; -; .
PhosphoSiteP02649; -; .
SWISS-2DPAGEP02649; -; .
DOSAC-COBS-2DPAGEP02649; -; .
PeptideAtlasP02649; -; .
PRIDEP02649; -; .
EnsemblENST00000252486; ENSP00000252486; ENSG00000130203; .
GeneID348; -; .
KEGGhsa:348; -; .
UCSCuc002pab.1; human; .
CTD348; -; .
GeneCardsGC19P041839; -; .
HGNCHGNC:613; APOE; .
HPACAB008363; -; .
MIM104310; phenotype; .
MIM107741; gene+phenotype; .
MIM269600; phenotype; .
MIM611771; phenotype; .
neXtProtNX_P02649; -; .
Orphanet238616; Alzheimer disease; .
Orphanet412; Hyperlipidemia type 3; .
Orphanet158029; Sea-blue histiocytosis; .
PharmGKBPA55; -; .
eggNOGprNOG10603; -; .
HOGENOMHBG126848; -; .
HOVERGENHBG010582; -; .
InParanoidP02649; -; .
OMAEMGSRTR; -; .
OrthoDBEOG4GB76S; -; .
PhylomeDBP02649; -; .
ReactomeREACT_22258; Metabolism of lipids and lipoproteins; .
DrugBankDB00062; Human Serum Albumin; .
DrugBankDB00064; Serum albumin iodonated; .
NextBio1435; -; .
PMAP-CutDBP02649; -; .
ArrayExpressP02649; -; .
BgeeP02649; -; .
CleanExHS_APOE; -; .
GenevestigatorP02649; -; .
GermOnlineENSG00000130203; Homo sapiens; .
GOGO:0042627; C:chylomicron; IDA:BHF-UCL; .
GOGO:0030425; C:dendrite; NAS:BHF-UCL; .
GOGO:0034364; C:high-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0034363; C:intermediate-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0034362; C:low-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0043025; C:neuronal cell body; NAS:BHF-UCL; .
GOGO:0034361; C:very-low-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0016209; F:antioxidant activity; IDA:BHF-UCL; .
GOGO:0001540; F:beta-amyloid binding; IDA:UniProtKB; .
GOGO:0008201; F:heparin binding; IDA:BHF-UCL; .
GOGO:0050750; F:low-density lipoprotein particle receptor binding; IDA:BHF-UCL; .
GOGO:0046911; F:metal chelating activity; IDA:BHF-UCL; .
GOGO:0060228; F:phosphatidylcholine-sterol O-acyltransferase activator activity; IDA:BHF-UCL; .
GOGO:0005543; F:phospholipid binding; IDA:BHF-UCL; .
GOGO:0046982; F:protein heterodimerization activity; IPI:BHF-UCL; .
GOGO:0042803; F:protein homodimerization activity; IDA:BHF-UCL; .
GOGO:0048156; F:tau protein binding; IPI:BHF-UCL; .
GOGO:0070326; F:very-low-density lipoprotein particle receptor binding; IDA:BHF-UCL; .
GOGO:0006916; P:anti-apoptosis; IDA:BHF-UCL; .
GOGO:0032488; P:Cdc42 protein signal transduction; IDA:BHF-UCL; .
GOGO:0008219; P:cell death; IEA:UniProtKB-KW; .
GOGO:0019934; P:cGMP-mediated signaling; IDA:BHF-UCL; .
GOGO:0033344; P:cholesterol efflux; IDA:BHF-UCL; .
GOGO:0042632; P:cholesterol homeostasis; IDA:BHF-UCL; .
GOGO:0034382; P:chylomicron remnant clearance; IMP:BHF-UCL; .
GOGO:0007010; P:cytoskeleton organization; TAS:UniProtKB; .
GOGO:0007186; P:G-protein coupled receptor protein signaling pathway; IDA:BHF-UCL; .
GOGO:0034380; P:high-density lipoprotein particle assembly; IDA:BHF-UCL; .
GOGO:0034384; P:high-density lipoprotein particle clearance; IDA:BHF-UCL; .
GOGO:0034375; P:high-density lipoprotein particle remodeling; IGI:BHF-UCL; .
GOGO:0006917; P:induction of apoptosis; IDA:UniProtKB; .
GOGO:0046907; P:intracellular transport; TAS:UniProtKB; .
GOGO:0043537; P:negative regulation of blood vessel endothelial cell migration; IDA:BHF-UCL; .
GOGO:0045541; P:negative regulation of cholesterol biosynthetic process; IDA:BHF-UCL; .
GOGO:0001937; P:negative regulation of endothelial cell proliferation; IDA:BHF-UCL; .
GOGO:0050728; P:negative regulation of inflammatory response; IC:BHF-UCL; .
GOGO:0043407; P:negative regulation of MAP kinase activity; IDA:BHF-UCL; .
GOGO:0010544; P:negative regulation of platelet activation; IDA:BHF-UCL; .
GOGO:0007263; P:nitric oxide mediated signal transduction; IDA:BHF-UCL; .
GOGO:0033700; P:phospholipid efflux; IDA:BHF-UCL; .
GOGO:0030828; P:positive regulation of cGMP biosynthetic process; IDA:BHF-UCL; .
GOGO:0010875; P:positive regulation of cholesterol efflux; IDA:BHF-UCL; .
GOGO:0010873; P:positive regulation of cholesterol esterification; IDA:BHF-UCL; .
GOGO:0032805; P:positive regulation of low-density lipoprotein particle receptor catabolic process; IDA:BHF-UCL; .
GOGO:0051044; P:positive regulation of membrane protein ectodomain proteolysis; IDA:BHF-UCL; .
GOGO:0051000; P:positive regulation of nitric-oxide synthase activity; IDA:BHF-UCL; .
GOGO:0006898; P:receptor-mediated endocytosis; IDA:BHF-UCL; .
GOGO:0030516; P:regulation of axon extension; TAS:UniProtKB; .
GOGO:0048168; P:regulation of neuronal synaptic plasticity; TAS:UniProtKB; .
GOGO:0000302; P:response to reactive oxygen species; NAS:UniProtKB; .
GOGO:0043691; P:reverse cholesterol transport; IDA:BHF-UCL; .
GOGO:0007271; P:synaptic transmission; cholinergic; TAS:UniProtKB
GOGO:0006641; P:triglyceride metabolic process; IDA:BHF-UCL; .
GOGO:0034447; P:very-low-density lipoprotein particle clearance; IDA:BHF-UCL; .
GOGO:0034372; P:very-low-density lipoprotein particle remodeling; IDA:BHF-UCL; .
InterProIPR013326; ApoA/E_ApoLp; .
InterProIPR000074; ApoA1_A4_E; .
Gene3DG3DSA:1.20.120.20; ApoA/E_ApoLp; 2; .
PfamPF01442; Apolipoprotein; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server