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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: ASSY_ECOLI |
SWISS-2DPAGE: ASSY_ECOLI
ASSY_ECOLI:
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ID ASSY_ECOLI; STANDARD; 2DG.
AC P0A6E4; P22767;
DT 01-OCT-1998, integrated into SWISS-2DPAGE (release 8).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName:
DE Full=Citrulline--aspartate ligase;.
GN Name=argG; OrderedLocusNames=b3172, JW3140;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0014XO=5.28/46220;
2D -!- PEPTIDE MASSES: SPOT 2D-0014XO: 910.38; 947.446; 1013.541;
2D 1094.461; 1127.469; 1282.61; 1296.673; 1372.633; 1701.924; 1920;
2D 2101.163; 2204.134; 2313.201; 2469.304; 2625.275; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001KZX=5.08/51667;
2D -!- PEPTIDE MASSES: SPOT 2D-001KZX: 910.432; 1013.56; 1094.494; 1111.5;
2D 1127.507; 1266.645; 1282.622; 1356.633; 1372.616; 1540.715; 1673.728;
2D 1701.901; 1903.939; 1919.932; 2101.029; 2204.07; 2313.108; 2625.184; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WK2=5.27/46667;
2D -!- PEPTIDE MASSES: SPOT 2D-001WK2: 910.4; 947.44; 955.49; 1013.52;
2D 1094.42; 1167.51; 1266.57; 1267.55; 1296.59; 1356.59; 1701.84; 1865.69;
2D 1903.88; 1978.97; 2203.99; 2286.02; 2313.03; 2469.11; 2625.04; 2781.13;
2D 2881.17; 3140.35; 3378.45; 3506.42; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WK2:
2D (K)GAVPYAYTANLGQPDEEDYDAIPR(R),34-57;
2D (R)YGLLTNAELQIYKPWLDTDFIDELGGR,142-168; (K)FWDESVKIPAEEVTVR(F),224-239;
2D (K)IPAEEVTVR(F),231-239; (R)LLTGIHNEDTIEQYHAHGR(Q),306-324;
2D (R)GNDYSILNTVSENLTYKPER(L),366-385; (R)NLDITDTR(E),408-415; (K)TGLLSSSAASGVPQVENLENK(G),424-444.
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [2].
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DR 2DBase-Ecoli; P0A6E4; ASSY_ECOLI.
DR UniProtKB/Swiss-Prot; P0A6E4; ASSY_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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