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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ATPA_ECOLI




SWISS-2DPAGE: ATPA_ECOLI



ATPA_ECOLI:

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ID   ATPA_ECOLI; STANDARD; 2DG.
AC   P0ABB0; P00822;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=ATP synthase subunit alpha; EC=3.6.3.14; AltName: Full=ATP
DE   synthase F1 sector subunit alpha; AltName: Full=F-ATPase subunit alpha;.
GN   Name=atpA; Synonyms=papA, uncA; OrderedLocusNames=b3734, JW3712;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI6-11, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: F-TYPE ATPASES HAVE 2 COMPONENTS, CF(1) AND CF(0) 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KQD=5.84/53178;
2D   -!-   PI/MW: SPOT 2D-000KYE=5.98/43139;
2D   -!-   MAPPING: SPOT 2D-000KQD: AMINO ACID COMPOSITION AND MICROSEQUENCE
2D         ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]; SPOT
2D         2D-000KYE: AMINO ACID COMPOSITION AND MICROSEQUENCING (MQLNSTEI) [3].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LDG=5.81/36727;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LDG: 882.533; 946.554; 1062.608;
2D         1074.568; 1298.724; 1537.692; 1722.776; 2149.925; 2641.087; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI6-11;
2D   -!-   PI/MW: SPOT 2D-001TDD=6.11/57637;
2D   -!-   PI/MW: SPOT 2D-001TNA=6.02/30657; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TDD: 882.303; 946.279; 1051.293;
2D         1058.268; 1062.343; 1183.391; 1270.349; 1298.46; 1349.374; 1451.438;
2D         1505.408; 1521.441; 1537.427; 1722.508; 1925.464; 1941.442; 2149.629; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001TNA: 882.634; 946.656; 1051.655;
2D         1058.618; 1062.719; 1074.678; 1183.732; 1298.901; 1349.819; 1521.909;
2D         2150.348; 2641.688; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WIG=5.82/52204;
2D   -!-   PI/MW: SPOT 2D-001WMM=5.15/39861; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WIG: 1062.6; 1074.55; 1183.68; 1270.66;
2D         1298.8; 1349.69; 1537.81; 1551.77; 1568.83; 1722.89; 1856.91; 1925.92;
2D         1941.92; 1984.99; 2125.09; 2150.1; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WMM: 882.42; 946.4; 953.33; 1051.36;
2D         1058.37; 1062.44; 1094.36; 1270.4; 1298.53; 1349.4; 1505.49; 1524.54;
2D         1537.46; 1722.52; 1856.51; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WMM: (-)MQLNSTEISELIK(Q),1-13;
2D         (R)IAQFNVVSEAHNEGTIVSVSDGVIR(I),16-40; (R)YAIALNLER(D),60-68;
2D         (R)VVNTLGAPIDGK(G),107-118; (K)GPLDHDGFSAVEAIAPGVIER(Q),119-139;
2D         (K)TALAIDAIINQR(D),176-187; (K)CIYVAIGQK(A),193-201;
2D         (R)DRGEDALIIYDDLSK(Q),251-265; (R)EAFPGDVFYLHSR(L),284-296;
2D         (R)VNAEYVEAFTK(G),304-314; (R)ELAAFSQFASDLDDATR(K),402-418;
2D         (R)ELAAFSQFASDLDDATRK(Q),402-419; (K)QYAPMSVAQQSLVLFAAER(G),435-453.
2D   -!-   MAPPING: Peptide mass fingerprinting [5]; SPOT 2D-001WMM: TANDEM
2D         MASS SPECTROMETRY [5].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; G051.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0ABB0; ATPA_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli(6-11)
ECOLI6-11
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server