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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: CALR_HUMAN




SWISS-2DPAGE: CALR_HUMAN



CALR_HUMAN:

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ID   CALR_HUMAN; STANDARD; 2DG.
AC   P27797;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   30-DEC-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 16.
DE   RecName: Full=Calreticulin; AltName: Full=CRP55; AltName:
DE   Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60;
DE   Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor;.
GN   Name=CALR; Synonyms=CRTC;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN, U937_HUMAN.
IM   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM   LIVER_HUMAN, LYMPHOCYTE_HUMAN, LYMPHOMA_HUMAN, PLATELET_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA   Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA   R.D., Hughes G.J.;
RT   ''''''Human liver protein map: a reference database established by
RT   microsequencing and gel comparison'';'';'';
RL   Electrophoresis 13(1):992-1001(1992).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant
RA   L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.;
RT   ''''''Human blood platelet protein map established by two-dimensional
RT   polyacrylamide gel electrophoresis'';'';'';
RL   Electrophoresis 16(1):1152-1159(1995).
RN   [4]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [5]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [6]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [7]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [8]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [9]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: SINGLE CHAIN 
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000TB6=4.40/58700;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [7].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001DPP=4.40/58700;
2D   -!-   MAPPING: MATCHING WITH THE HEPG2 MASTER GEL [8].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000E1E=4.40/60638;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0002K8=4.36/59311;
2D   -!-   PI/MW: SPOT 2D-0002KB=4.40/58700;
2D   -!-   PI/MW: SPOT 2D-0002KE=4.42/58700;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000YMC=4.33/57239;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [5][6].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000MS2=4.40/58560;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000MS2: B=15, Z=16.5, S=4.4, H=1,
2D         G=9.4, T=3.2, A=3.9, P=5.1, Y=10.4, R=2.2, V=4.7, M=0.5, I=4.3, L=4.8,
2D         F=6.3, K=8.2;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID
2D         COMPOSITION ANALYSIS [4].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0000K7=4.40/58795;
2D   -!-   PATHOLOGICAL LEVEL: INCREASED DURING HYPERCALCEMIA.
2D   -!-   MAPPING: MICROSEQUENCING [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001XX7=4.29/44500;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001XX7: 853.434; 887.3898; 906.4702;
2D         927.4915; 975.4933; 1019.5579; 1045.564; 1047.5083; 1130.5341; 1147.6566;
2D         1219.6814; 1410.6379; 1476.6938; 1607.7465; 1800.8494; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [9].
2D   -!- MASTER: LYMPHOMA_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0007EP=4.40/59000;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: PLATELET_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000FLH=4.41/61232;
2D   -!-   PI/MW: SPOT 2D-000FM6=4.40/58200;
2D   -!-   PI/MW: SPOT 2D-000FMG=4.46/58200;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [3].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000CRG=3.73/58084;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
CC   Usage by and for commercial entities requires a license agreement (See
CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P27797; P27797.
DR   DOSAC-COBS-2DPAGE; P27797; CALR_HUMAN.
DR   HSC-2DPAGE; P27797; HUMAN.
DR   OGP-WWW; P27797.
DR   PHCI-2DPAGE; P27797; CRTC_HUMAN.
DR   PMMA-2DPAGE; P27797; CRTC_HUMAN.
DR   REPRODUCTION-2DPAGE; IPI00020599; IPI00020599.
DR   Siena-2DPAGE; P27797; CALR_HUMAN.
DR   UCD-2DPAGE; P27797; CALR_HUMAN.
DR   UniProtKB/Swiss-Prot; P27797; CALR_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal epithelia cells
CEC_HUMAN
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
HepG2
HEPG2_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
Lymphoma
LYMPHOMA_HUMAN
Platelet
PLATELET_HUMAN
Macrophage Like Cell Line
U937_HUMAN
 


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server