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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: CH60_ECOLI SWISS-2DPAGE:  CH60_ECOLI CH60_ECOLI General information about the entry View entry in simple text format Entry name CH60_ECOLI Primary accession number P0A6F5 Secondary accession number(s) P06139 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on March 31, 2004 (version 5) General Annotations were last modified on  May 19, 2011 (version 14) Name and origin of the protein Description RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60;. Gene name Name=groL Synonyms=groEL, mopA OrderedLocusNames=b4143, JW4103 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   PROTEIN EXPRESSION MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan] Gage D.J., Neidhardt F.C. ''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';'' J. Bacteriol. 175(1):7105-7108(1993) [4]   PROTEIN EXPRESSION MEDLINE=87083376; PubMed=3539918; [NCBI, Expasy, EBI, Israel, Japan] Vanbogelen R.A., Kelley P.M., Neidhardt F.C. ''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';'' J. Bacteriol. 169(1):26-32(1987) [5]   MAPPING ON GEL PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''''''New perspectives in the Escherichia coli proteome investigation'';'';'' Proteomics 1(1):409-423(2001) [6]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) Comments SUBUNIT: OLIGOMER OF 14 SUBUNITS COMPOSED OF TWO STACKED RINGS OF 7 SUBUNITS 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000KMU: pI=4.85; Mw=56743 EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4]. MAPPING (identification): AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]. ECOLI4-5 {Escherichia coli(4-5)} Escherichia coli   map experimental info   protein estimated location   ECOLI4-5 MAP LOCATIONS: SPOT 2D-001HFT: pI=4.97; Mw=61125   [identification data] SPOT 2D-001HGK: pI=4.99; Mw=56877   [identification data] SPOT 2D-001HGR: pI=4.93; Mw=55185   [identification data] SPOT 2D-001HGT: pI=4.95; Mw=55185   [identification data] SPOT 2D-001HGU: pI=4.95; Mw=55185   [identification data] SPOT 2D-001HH2: pI=4.84; Mw=52802   [identification data] MAPPING (identification): Peptide mass fingerprinting [5]; SPOTS 1HGK, 1HGU: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [5]. ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI4.5-5.5 MAP LOCATIONS: SPOT 2D-0014UQ: pI=4.99; Mw=55445   [identification data] SPOT 2D-0014V3: pI=4.96; Mw=54287   [identification data] MAPPING (identification): Peptide mass fingerprinting [5]; SPOT 2D-0014V3: MICROSEQUENCING (XLRG) [5]. ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WFT: pI=4.93; Mw=61278   [identification data] SPOT 2D-001WFW: pI=4.96; Mw=59987   [identification data] SPOT 2D-001WG2: pI=4.98; Mw=60758   [identification data] MAPPING (identification): Peptide mass fingerprinting [6]; SPOT 2D-001WFW: TANDEM MASS SPECTROMETRY [6]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references 2DBase-Ecoli P0A6F5; CH60_ECOLI. ECO2DBASE B056.5; 6TH EDITION. UniProtKB/Swiss-Prot P0A6F5; CH60_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name CH60_ECOLI Primary accession number P0A6F5 Secondary accession number(s) P06139 Q2M6G1 Sequence was last modified on  January 23, 2007 (version 2) Annotations were last modified on  October 19, 2011 (version 79) Name and origin of the protein Description RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60; Gene name Name=groL Synonyms=groEL, mopA OrderedLocusNames=b4143, JW4103 Encoded on Name=groL; Synonyms=groEL, mopA; OrderedLocusNames=b4143, JW4103 Keywords 3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X07850; CAA30698.1; -; Genomic_DNA EMBL U14003; AAA97042.1; -; Genomic_DNA EMBL U00096; AAC77103.1; -; Genomic_DNA EMBL AP009048; BAE78145.1; -; Genomic_DNA EMBL X07899; CAA30739.1; -; Genomic_DNA EMBL M11294; AAA23934.1; ALT_FRAME; Genomic_DNA PIR S56371; BVECGL; . RefSeq NP_418567.1; NC_000913.2; . PDB 1AON; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548 PDB 1DK7; X-ray; 2.02 A; A/B=191-336 PDB 1DKD; X-ray; 2.10 A; A/B/C/D=191-336 PDB 1FY9; X-ray; 2.20 A; A=191-376 PDB 1FYA; X-ray; 2.20 A; A=191-376 PDB 1GR5; EM; 7.90 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548 PDB 1GRL; X-ray; 2.80 A; A/B/C/D/E/F/G=1-548 PDB 1GRU; EM; 12.50 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548 PDB 1J4Z; X-ray; 3.52 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 1JON; X-ray; 2.50 A; A=191-345 PDB 1KID; X-ray; 1.70 A; A=191-375 PDB 1KP8; X-ray; 2.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 1KPO; X-ray; 3.52 A; 1/2/O/P/Q/R/S/T/U/V/W/X/Y/Z=2-547 PDB 1LA1; X-ray; 2.06 A; A=188-379 PDB 1MNF; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 1OEL; X-ray; 2.80 A; A/B/C/D/E/F/G=2-548 PDB 1PCQ; X-ray; 2.81 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524 PDB 1PF9; X-ray; 2.99 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-525 PDB 1SS8; X-ray; 2.70 A; A/B/C/D/E/F/G=2-524 PDB 1SVT; X-ray; 2.81 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524 PDB 1SX3; X-ray; 2.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-525 PDB 1SX4; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524 PDB 1XCK; X-ray; 2.92 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-548 PDB 2C7C; EM; 7.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 2C7D; EM; 8.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 2C7E; EM; 9.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548 PDB 2CGT; EM; 8.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547 PDB 2EU1; X-ray; 3.29 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-548 PDB 2NWC; X-ray; 3.02 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548 PDB 2YEY; X-ray; 3.30 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-525 PDB 3C9V; EM; 4.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-527 PDB 3CAU; EM; 4.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-527 PDBsum 1AON; -; . PDBsum 1DK7; -; . PDBsum 1DKD; -; . PDBsum 1FY9; -; . PDBsum 1FYA; -; . PDBsum 1GR5; -; . PDBsum 1GRL; -; . PDBsum 1GRU; -; . PDBsum 1J4Z; -; . PDBsum 1JON; -; . PDBsum 1KID; -; . PDBsum 1KP8; -; . PDBsum 1KPO; -; . PDBsum 1LA1; -; . PDBsum 1MNF; -; . PDBsum 1OEL; -; . PDBsum 1PCQ; -; . PDBsum 1PF9; -; . PDBsum 1SS8; -; . PDBsum 1SVT; -; . PDBsum 1SX3; -; . PDBsum 1SX4; -; . PDBsum 1XCK; -; . PDBsum 2C7C; -; . PDBsum 2C7D; -; . PDBsum 2C7E; -; . PDBsum 2CGT; -; . PDBsum 2EU1; -; . PDBsum 2NWC; -; . PDBsum 2YEY; -; . PDBsum 3C9V; -; . PDBsum 3CAU; -; . ProteinModelPortal P0A6F5; -; . SMR P0A6F5; 2-525; . DIP DIP-339N; -; . IntAct P0A6F5; 690; . MINT MINT-5232496; -; . SWISS-2DPAGE P0A6F5; -; . 2DBase-Ecoli P0A6F5; -; . ECO2DBASE B056.5; 6TH EDITION; . PRIDE P0A6F5; -; . EnsemblBacteria EBESCT00000003358; EBESCP00000003358; EBESCG00000002749; . EnsemblBacteria EBESCT00000017770; EBESCP00000017061; EBESCG00000016826; . GeneID 948665; -; . GenomeReviews AP009048_GR; JW4103; . GenomeReviews U00096_GR; b4143; . KEGG ecj:JW4103; -; . KEGG eco:b4143; -; . EchoBASE EB0594; -; . EcoGene EG10599; groL; . eggNOG COG0459; -; . GeneTree EBGT00050000009756; -; . HOGENOM HBG625289; -; . OMA DKPAAGM; -; . ProtClustDB PRK00013; -; . BioCyc EcoCyc:EG10599-MONOMER; -; . Genevestigator P0A6F5; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0005515; F:protein binding; IPI:IntAct; . GO GO:0007049; P:cell cycle; IEA:UniProtKB-KW; . GO GO:0051301; P:cell division; IEA:UniProtKB-KW; . GO GO:0042026; P:protein refolding; IEA:InterPro; . HAMAP MF_00600; CH60; 1; - InterPro IPR018370; Chaperonin_Cpn60_CS; . InterPro IPR001844; Chaprnin_Cpn60; . InterPro IPR002423; Cpn60/TCP-1; . PANTHER PTHR11353; Cpn60/TCP-1; 1; . Pfam PF00118; Cpn60_TCP1; 1; . PRINTS PR00298; CHAPERONIN60; . SUPFAM SSF48592; GroEL-ATPase; 1; . TIGRFAMs TIGR02348; GroEL; 1; . PROSITE PS00296; CHAPERONINS_CPN60; 1; . SWISS-2DPAGE (search AC)