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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: CLUS_HUMAN




SWISS-2DPAGE:  CLUS_HUMAN


CLUS_HUMAN


General information about the entry
View entry in simple text format
Entry nameCLUS_HUMAN
Primary accession numberP10909
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 30, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 16)
Name and origin of the protein
DescriptionRecName: Full=Clusterin; AltName: Full=Aging-associated gene 4 protein; AltName: Full=Apolipoprotein J; Short=Apo-J; AltName: Full=Complement cytolysis inhibitor; Short=CLI; AltName: Full=Complement-associated protein SP-40,40; AltName: Full=Ku70-binding protein 1; AltName: Full=NA1/NA2; AltName: Full=Testosterone-repressed prostate message 2; Short=TRPM-2; Contains: RecName: Full=Clusterin beta chain; AltName: Full=ApoJalpha; AltName: Full=Complement cytolysis inhibitor a chain; Contains: RecName: Full=Clusterin alpha chain; AltName: Full=ApoJbeta; AltName: Full=Complement cytolysis inhibitor b chain; Flags: Precursor;.
Gene nameName=CLU
Synonyms=APOJ, CLI, KUB1
ORFNames=AAG4
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.
''''''Plasma protein map: an update by microsequencing'';'';''
Electrophoresis 13(1):707-714(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: NA1 AND NA2 CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=4.65; Mw=39035
pI=4.73; Mw=38269
pI=4.82; Mw=37892
pI=5.04; Mw=36691
pI=5.14; Mw=36061
pI=4.75; Mw=36782
pI=4.82; Mw=36782
pI=4.77; Mw=35705
pI=4.85; Mw=35883
pI=5.28; Mw=35353
pI=4.93; Mw=35705
pI=5.08; Mw=35266
pI=4.86; Mw=35092
pI=4.95; Mw=35005
pI=5.45; Mw=34403
pI=5.60; Mw=34233
pI=5.04; Mw=34318
pI=5.67; Mw=34233
pI=4.95; Mw=34064
pI=5.14; Mw=33980
pI=5.15; Mw=33149
pI=5.08; Mw=33314
pI=5.60; Mw=33149
pI=5.66; Mw=32099
pI=5.60; Mw=32019

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=4.66; Mw=33819
pI=4.74; Mw=33585
pI=4.84; Mw=33276
pI=4.96; Mw=33046
pI=4.68; Mw=32441
pI=4.75; Mw=32366
pI=4.87; Mw=31700

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=4.73; Mw=39537
pI=4.73; Mw=38905
pI=4.78; Mw=38827
pI=4.93; Mw=38827
pI=4.83; Mw=38827
pI=4.84; Mw=37976
pI=4.89; Mw=37900
pI=4.95; Mw=38053
pI=4.85; Mw=37219
pI=4.96; Mw=37445
pI=4.89; Mw=37219
pI=4.81; Mw=37069
pI=4.96; Mw=36920
pI=4.91; Mw=36550
pI=4.96; Mw=36477
pI=4.99; Mw=35750
pI=5.07; Mw=35178

MAPPING (identification):
MICROSEQUENCING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
DOSAC-COBS 2D-PAGEP10909; P10909.
DOSAC-COBS-2DPAGEP10909; CLUS_HUMAN.
OGP-WWWP10909.
REPRODUCTION-2DPAGEIPI00291262; IPI00291262.
Siena-2DPAGEP10909; CLUS_HUMAN.
UniProtKB/Swiss-ProtP10909; CLUS_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCLUS_HUMAN
Primary accession numberP10909
Secondary accession number(s) B2R9Q1 B3KSE6 P11380 P11381 Q2TU75 Q5HYC1 Q7Z5B9
Sequence was last modified on July 1, 1989 (version 1)
Annotations were last modified on October 19, 2011 (version 141)
Name and origin of the protein
DescriptionRecName: Full=Clusterin; AltName: Full=Aging-associated gene 4 protein; AltName: Full=Apolipoprotein J; Short=Apo-J; AltName: Full=Complement cytolysis inhibitor; Short=CLI; AltName: Full=Complement-associated protein SP-40,40; AltName: Full=Ku70-binding protein 1; AltName: Full=NA1/NA2; AltName: Full=Testosterone-repressed prostate message 2; Short=TRPM-2; Contains: RecName: Full=Clusterin beta chain; AltName: Full=ApoJalpha; AltName: Full=Complement cytolysis inhibitor a chain; Contains: RecName: Full=Clusterin alpha chain; AltName: Full=ApoJbeta; AltName: Full=Complement cytolysis inhibitor b chain; Flags: Precursor;
Gene nameName=CLU
Synonyms=APOJ, CLI, KUB1
ORFNames=AAG4
Encoded onName=CLU; Synonyms=APOJ, CLI, KUB1; ORFNames=AAG4
KeywordsAlternative splicing; Apoptosis; Chaperone; Complement pathway; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Immunity; Innate immunity; Membrane; Microsome; Mitochondrion; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Secreted; Signal; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM25915; AAA35692.1; ALT_INIT; mRNA
EMBLM63379; AAB06507.1; -; Genomic_DNA
EMBLM63376; AAB06507.1; JOINED; Genomic_DNA
EMBLM63377; AAB06507.1; JOINED; Genomic_DNA
EMBLM63378; AAB06507.1; JOINED; Genomic_DNA
EMBLM64722; AAB06508.1; ALT_SEQ; mRNA
EMBLAK093399; BAG52708.1; -; mRNA
EMBLAK313870; BAG36598.1; ALT_INIT; mRNA
EMBLCR599675; -; NOT_ANNOTATED_CDS; mRNA
EMBLBX648414; CAI45990.1; -; mRNA
EMBLAY341244; AAP88927.1; ALT_INIT; Genomic_DNA
EMBLAF311103; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBC010514; AAH10514.1; ALT_INIT; mRNA
EMBLBC019588; AAH19588.1; ALT_INIT; mRNA
EMBLBU150467; -; NOT_ANNOTATED_CDS; mRNA
EMBLJ02908; AAA51765.1; -; mRNA
EMBLM74816; AAA60321.1; -; mRNA
EMBLL00974; AAA60567.1; -; Genomic_DNA
EMBLX14723; CAA32847.1; ALT_INIT; mRNA
EMBLAY513288; AAT08041.1; ALT_INIT; mRNA
IPIIPI00291262; -; .
IPIIPI00400826; -; .
IPIIPI00954954; -; .
IPIIPI00976752; -; .
IPIIPI00978715; -; .
PIRS43646; A41386; .
RefSeqNP_976084.1; NM_203339.2; .
UniGeneHs.436657; -; .
ProteinModelPortalP10909; -; .
IntActP10909; 16; .
MINTMINT-3007494; -; .
STRINGP10909; -; .
PhosphoSiteP10909; -; .
SWISS-2DPAGEP10909; -; .
Cornea-2DPAGEP10909; -; .
DOSAC-COBS-2DPAGEP10909; -; .
OGPP10909; -; .
REPRODUCTION-2DPAGEIPI00291262; -; .
PRIDEP10909; -; .
EnsemblENST00000316403; ENSP00000315130; ENSG00000120885; .
EnsemblENST00000380446; ENSP00000369812; ENSG00000120885; .
EnsemblENST00000405140; ENSP00000385419; ENSG00000120885; .
GeneID1191; -; .
KEGGhsa:1191; -; .
UCSCuc003xfw.1; human; .
CTD1191; -; .
GeneCardsGC08M025999; -; .
H-InvDBHIX0007408; -; .
HGNCHGNC:2095; CLU; .
HPACAB000476; -; .
HPACAB016253; -; .
HPAHPA000572; -; .
MIM185430; gene; .
neXtProtNX_P10909; -; .
PharmGKBPA26620; -; .
eggNOGprNOG17094; -; .
HOVERGENHBG006908; -; .
InParanoidP10909; -; .
OMANGDRIDS; -; .
OrthoDBEOG447FTH; -; .
ReactomeREACT_604; Hemostasis; .
NextBio4922; -; .
PMAP-CutDBP10909; -; .
ArrayExpressP10909; -; .
BgeeP10909; -; .
CleanExHS_CLU; -; .
GenevestigatorP10909; -; .
GermOnlineENSG00000120885; Homo sapiens; .
GOGO:0042583; C:chromaffin granule; IEA:UniProtKB-SubCell; .
GOGO:0005829; C:cytosol; IEA:UniProtKB-SubCell; .
GOGO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell; .
GOGO:0005792; C:microsome; IEA:UniProtKB-SubCell; .
GOGO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB; .
GOGO:0031093; C:platelet alpha granule lumen; TAS:Reactome; .
GOGO:0034366; C:spherical high-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0051787; F:misfolded protein binding; IDA:UniProtKB; .
GOGO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB; .
GOGO:0061077; P:chaperone-mediated protein folding; IDA:UniProtKB; .
GOGO:0006958; P:complement activation; classical pathway; IEA:UniProtKB-KW
GOGO:0008629; P:induction of apoptosis by intracellular signals; IDA:UniProtKB; .
GOGO:0045087; P:innate immune response; IEA:UniProtKB-KW; .
GOGO:0006629; P:lipid metabolic process; NAS:ProtInc; .
GOGO:0032463; P:negative regulation of protein homooligomerization; IMP:BHF-UCL; .
GOGO:0030168; P:platelet activation; TAS:Reactome; .
GOGO:0002576; P:platelet degranulation; TAS:Reactome; .
GOGO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IMP:UniProtKB; .
GOGO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB; .
GOGO:2000060; P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IMP:UniProtKB; .
GOGO:0050821; P:protein stabilization; IDA:UniProtKB; .
GOGO:0001836; P:release of cytochrome c from mitochondria; IC:BHF-UCL; .
GOGO:0051788; P:response to misfolded protein; IDA:BHF-UCL; .
GOGO:0009615; P:response to virus; IEP:UniProtKB; .
GOGO:0043691; P:reverse cholesterol transport; TAS:BHF-UCL; .
InterProIPR016016; Clusterin; .
InterProIPR000753; Clusterin-like; .
InterProIPR016015; Clusterin_C; .
InterProIPR016014; Clusterin_N; .
PfamPF01093; Clusterin; 1; .
PIRSFPIRSF002368; Clusterin; 1; .
SMARTSM00035; CLa; 1; .
SMARTSM00030; CLb; 1; .
PROSITEPS00492; CLUSTERIN_1; 1; .
PROSITEPS00493; CLUSTERIN_2; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server