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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: DAPA_ECOLI SWISS-2DPAGE:  DAPA_ECOLI DAPA_ECOLI General information about the entry View entry in simple text format Entry name DAPA_ECOLI Primary accession number P0A6L2 Secondary accession number(s) P05640 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on March 31, 2004 (version 2) General Annotations were last modified on  May 19, 2011 (version 14) Name and origin of the protein Description RecName: Full=Dihydrodipicolinate synthase; Short=DHDPS; EC=4.2.1.52;. Gene name Name=dapA OrderedLocusNames=b2478, JW2463 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   MAPPING ON GEL MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F. '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'' Electrophoresis 19(1):1960-1971(1998) Comments SUBUNIT: HOMOTETRAMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000L8E: pI=5.94; Mw=32970   [identification data] SPOT 2D-000L8W: pI=6.09; Mw=32408 MAPPING (identification): SPOT 2D-000L8E: AMINO ACID COMPOSITION AND SEQUENCE TAG (MFTG) [3]; SPOT 2D-000L8W: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE G031.3; 6TH EDITION. UniProtKB/Swiss-Prot P0A6L2; DAPA_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name DAPA_ECOLI Primary accession number P0A6L2 Secondary accession number(s) P05640 P78223 Sequence was last modified on  May 10, 2005 (version 1) Annotations were last modified on  October 19, 2011 (version 65) Name and origin of the protein Description RecName: Full=Dihydrodipicolinate synthase; Short=DHDPS; EC=4.2.1.52; Gene name Name=dapA OrderedLocusNames=b2478, JW2463 Encoded on Name=dapA; OrderedLocusNames=b2478, JW2463 Keywords 3D-structure; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Diaminopimelate biosynthesis; Direct protein sequencing; Lyase; Lysine biosynthesis; Reference proteome; Schiff base. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M12844; AAA23665.1; -; Genomic_DNA EMBL U00096; AAC75531.1; -; Genomic_DNA EMBL AP009048; BAA16355.1; -; Genomic_DNA EMBL M33928; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL X57402; CAA40660.1; -; Genomic_DNA PIR E65023; SYECDP; . RefSeq NP_416973.1; NC_000913.2; . PDB 1DHP; X-ray; 2.30 A; A/B=1-292 PDB 1S5T; X-ray; 2.30 A; A/B=1-292 PDB 1S5V; X-ray; 2.35 A; A/B=1-292 PDB 1S5W; X-ray; 2.32 A; A/B=1-292 PDB 1YXC; X-ray; 1.90 A; A/B=1-292 PDB 1YXD; X-ray; 2.00 A; A/B=1-292 PDB 2A6L; X-ray; 2.05 A; A/B=1-292 PDB 2A6N; X-ray; 1.94 A; A/B=1-292 PDB 2ATS; X-ray; 1.90 A; A/B=1-292 PDB 2OJP; X-ray; 1.70 A; A/B=1-292 PDB 2PUR; X-ray; 1.70 A; A/B=1-292 PDB 3C0J; X-ray; 2.40 A; A/B=1-292 PDB 3DEN; X-ray; 2.20 A; A/B=1-292 PDB 3DU0; X-ray; 2.00 A; A/B=1-292 PDB 3I7Q; X-ray; 2.00 A; A/B=1-292 PDB 3I7R; X-ray; 2.10 A; A/B=1-292 PDB 3I7S; X-ray; 2.30 A; A/B=1-292 PDBsum 1DHP; -; . PDBsum 1S5T; -; . PDBsum 1S5V; -; . PDBsum 1S5W; -; . PDBsum 1YXC; -; . PDBsum 1YXD; -; . PDBsum 2A6L; -; . PDBsum 2A6N; -; . PDBsum 2ATS; -; . PDBsum 2OJP; -; . PDBsum 2PUR; -; . PDBsum 3C0J; -; . PDBsum 3DEN; -; . PDBsum 3DU0; -; . PDBsum 3I7Q; -; . PDBsum 3I7R; -; . PDBsum 3I7S; -; . ProteinModelPortal P0A6L2; -; . SMR P0A6L2; 1-292; . IntAct P0A6L2; 3; . SWISS-2DPAGE P0A6L2; -; . ECO2DBASE G031.3; 6TH EDITION; . EnsemblBacteria EBESCT00000003863; EBESCP00000003863; EBESCG00000003158; . EnsemblBacteria EBESCT00000017618; EBESCP00000016909; EBESCG00000016674; . GeneID 946952; -; . GenomeReviews AP009048_GR; JW2463; . GenomeReviews U00096_GR; b2478; . KEGG ecj:JW2463; -; . KEGG eco:b2478; -; . EchoBASE EB0201; -; . EcoGene EG10205; dapA; . eggNOG COG0329; -; . GeneTree EBGT00050000008975; -; . HOGENOM HBG358848; -; . OMA FMLCGGH; -; . ProtClustDB PRK03170; -; . BioCyc EcoCyc:DIHYDRODIPICSYN-MONOMER; -; . BioCyc MetaCyc:DIHYDRODIPICSYN-MONOMER; -; . Genevestigator P0A6L2; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0008840; F:dihydrodipicolinate synthase activity; IDA:EcoliWiki; . GO GO:0019877; P:diaminopimelate biosynthetic process; IDA:EcoliWiki; . HAMAP MF_00418; DapA; 1; - InterPro IPR013785; Aldolase_TIM; . InterPro IPR002220; Dihydrodipicolinate_synth-like; . InterPro IPR020625; Dihydrodipicolinate_synth_AS; . InterPro IPR020624; Dihydrodipicolinate_synth_CS; . InterPro IPR005263; Dihydrodipicolinate_synth_DapA; . Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 1; . PANTHER PTHR12128; DHDPS; 1; . Pfam PF00701; DHDPS; 1; . PIRSF PIRSF001365; DHDPS; 1; . PRINTS PR00146; DHPICSNTHASE; . TIGRFAMs TIGR00674; DapA; 1; . PROSITE PS00665; DHDPS_1; 1; . PROSITE PS00666; DHDPS_2; 1; . SWISS-2DPAGE (search AC)