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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DHE3_MOUSE




SWISS-2DPAGE:  DHE3_MOUSE


DHE3_MOUSE


General information about the entry
View entry in simple text format
Entry nameDHE3_MOUSE
Primary accession numberP26443
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor;.
Gene nameName=Glud1
Synonyms=Glud
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=6.59; Mw=50530
pI=6.74; Mw=50688

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [1].



ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=6.82; Mw=54135  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.47; Mw=52035  [identification data]
pI=6.56; Mw=51747  [identification data]
pI=6.77; Mw=51174
pI=6.69; Mw=51459  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



NUCLEI_LIVER_MOUSE {Soluble nuclear proteins and matrix from liver tissue}
Mus musculus (Mouse)
Tissue: Liver
NUCLEI_LIVER_MOUSE
  map experimental info
  protein estimated location
 
NUCLEI_LIVER_MOUSE

MAP LOCATIONS:
pI=6.92; Mw=51569  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=6.59; Mw=51167
pI=6.71; Mw=51007

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP26443; P26443.
UCD-2DPAGEP26443; DHE3_MOUSE.
UniProtKB/Swiss-ProtP26443; DHE3_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDHE3_MOUSE
Primary accession numberP26443
Secondary accession number(s) Q8C273
Sequence was last modified on August 1, 1992 (version 1)
Annotations were last modified on September 21, 2011 (version 111)
Name and origin of the protein
DescriptionRecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor;
Gene nameName=Glud1
Synonyms=Glud
Encoded onName=Glud1; Synonyms=Glud
KeywordsAcetylation; ATP-binding; Complete proteome; Direct protein sequencing; GTP-binding; Mitochondrion; NADP; Nucleotide-binding; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX57024; CAA40341.1; -; mRNA
EMBLAK089152; BAC40767.1; -; mRNA
EMBLBC052724; AAH52724.1; -; mRNA
EMBLBC057347; AAH57347.1; -; mRNA
IPIIPI00114209; -; .
PIRS16239; S16239; .
RefSeqNP_032159.1; NM_008133.4; .
UniGeneMm.10600; -; .
ProteinModelPortalP26443; -; .
SMRP26443; 63-558; .
IntActP26443; 4; .
STRINGP26443; -; .
PhosphoSiteP26443; -; .
SWISS-2DPAGEP26443; -; .
REPRODUCTION-2DPAGEP26443; -; .
UCD-2DPAGEP26443; -; .
PRIDEP26443; -; .
EnsemblENSMUST00000022322; ENSMUSP00000022322; ENSMUSG00000021794; .
EnsemblENSMUST00000171067; ENSMUSP00000131223; ENSMUSG00000091316; .
GeneID14661; -; .
KEGGmmu:14661; -; .
NMPDRfig|10090.3.peg.28839; -; .
UCSCuc007tas.2; mouse; .
CTD2746; -; .
MGIMGI:95753; Glud1; .
eggNOGroNOG05123; -; .
HOGENOMHBG590661; -; .
HOVERGENHBG005479; -; .
InParanoidP26443; -; .
OMACIGVIEH; -; .
OrthoDBEOG4H72B7; -; .
PhylomeDBP26443; -; .
NextBio286534; -; .
ArrayExpressP26443; -; .
BgeeP26443; -; .
GenevestigatorP26443; -; .
GermOnlineENSMUSG00000021794; Mus musculus; .
GOGO:0005743; C:mitochondrial inner membrane; IDA:MGI; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004353; F:glutamate dehydrogenase [NAD(P)+] activity; IDA:MGI; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0005515; F:protein binding; IPI:MGI; .
GOGO:0006520; P:cellular amino acid metabolic process; IEA:InterPro; .
GOGO:0032024; P:positive regulation of insulin secretion; IGI:MGI; .
InterProIPR006095; Glu/Leu/Phe/Val_DH; .
InterProIPR006096; Glu/Leu/Phe/Val_DH_C; .
InterProIPR006097; Glu/Leu/Phe/Val_DH_dimer_dom; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF00208; ELFV_dehydrog; 1; .
PfamPF02812; ELFV_dehydrog_N; 1; .
PRINTSPR00082; GLFDHDRGNASE; .
SMARTSM00839; ELFV_dehydrog; 1; .
PROSITEPS00074; GLFV_DEHYDROGENASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server