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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: DHE4_ECOLI |
SWISS-2DPAGE: DHE4_ECOLI
DHE4_ECOLI:
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ID DHE4_ECOLI; STANDARD; 2DG.
AC P00370;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=NADP-specific glutamate dehydrogenase; Short=NADP-GDH; EC=1.4.1.4;.
GN Name=gdhA; OrderedLocusNames=b1761, JW1750;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMOHEXAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KWE=5.96/45417;
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WK7=5.81/46385;
2D -!- PI/MW: SPOT 2D-001WKM=5.94/44524;
2D -!- PEPTIDE MASSES: SPOT 2D-001WK7: 1116.58; 1148.46; 1175.64; 1196.66;
2D 1375.71; 1531.8; 1899.97; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WKM: 888.41; 1116.49; 1196.53; 1254.62;
2D 1375.58; 1531.67; 1608.71; 1706.75; 1740.8; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WKM: (R)NQIQVNR(A),72-78;
2D (R)VQFSSAIGPYK(G),82-92; (K)FLGFEQTFK(N),108-116; (K)LSNNTACVFTGK(G),186-197.
2D -!- MAPPING: Peptide mass fingerprinting [3]; SPOT 2D-001WKM: TANDEM
2D MASS SPECTROMETRY [3].
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DR ECO2DBASE; G043.6; 6TH EDITION.
DR ECO2DBASE; G043.7; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P00370; DHE4_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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