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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DKSA_ECOLI




SWISS-2DPAGE:  DKSA_ECOLI


DKSA_ECOLI


General information about the entry
View entry in simple text format
Entry nameDKSA_ECOLI
Primary accession numberP0ABS1
Secondary accession number(s) P18274
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=DnaK suppressor protein;.
Gene nameName=dksA
OrderedLocusNames=b0145, JW0141
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.89; Mw=18709
pI=5.01; Mw=17828

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (MQEG) [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.08; Mw=19656  [identification data]

MAPPING (identification):
Tandem mass spectrometry [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP0ABS1; DKSA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDKSA_ECOLI
Primary accession numberP0ABS1
Secondary accession number(s) P18274
Sequence was last modified on October 25, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 56)
Name and origin of the protein
DescriptionRecName: Full=DnaK suppressor protein;
Gene nameName=dksA
OrderedLocusNames=b0145, JW0141
Encoded onName=dksA; OrderedLocusNames=b0145, JW0141
Keywords3D-structure; Complete proteome; Direct protein sequencing; Metal-binding; Reference proteome; Zinc; Zinc-finger.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM34945; AAA23687.1; -; Genomic_DNA
EMBLU00096; AAC73256.1; -; Genomic_DNA
EMBLAP009048; BAB96722.1; -; Genomic_DNA
EMBLM60726; AAA72978.1; -; Genomic_DNA
PIRS45214; S45214; .
RefSeqNP_414687.1; NC_000913.2; .
PDB1TJL; X-ray; 2.00 A; A/B/C/D/E/F/G/H/I/J=1-151
PDBsum1TJL; -; .
ProteinModelPortalP0ABS1; -; .
SMRP0ABS1; 7-151; .
DIPDIP-31875N; -; .
IntActP0ABS1; 9; .
SWISS-2DPAGEP0ABS1; -; .
EnsemblBacteriaEBESCT00000000809; EBESCP00000000809; EBESCG00000000675; .
EnsemblBacteriaEBESCT00000014586; EBESCP00000013877; EBESCG00000013647; .
GeneID944850; -; .
GenomeReviewsAP009048_GR; JW0141; .
GenomeReviewsU00096_GR; b0145; .
KEGGecj:JW0141; -; .
KEGGeco:b0145; -; .
EchoBASEEB0226; -; .
EcoGeneEG10230; dksA; .
eggNOGCOG1734; -; .
GeneTreeEBGT00050000011460; -; .
HOGENOMHBG728818; -; .
OMAVDCKTLA; -; .
ProtClustDBPRK10778; -; .
BioCycEcoCyc:EG10230-MONOMER; -; .
GenevestigatorP0ABS1; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
InterProIPR012784; DksA_RNA_pol-bd; .
InterProIPR020460; Znf_C4-type_bac; .
InterProIPR000962; Znf_DskA_TraR; .
InterProIPR020458; Znf_DskA_TraR_CS; .
PfamPF01258; zf-dskA_traR; 1; .
PRINTSPR00618; DKSAZNFINGER; .
TIGRFAMsTIGR02420; DksA; 1; .
PROSITEPS01102; ZF_DKSA_1; 1; .
PROSITEPS51128; ZF_DKSA_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server