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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DNAB_ECOLI




SWISS-2DPAGE:  DNAB_ECOLI


DNAB_ECOLI


General information about the entry
View entry in simple text format
Entry nameDNAB_ECOLI
Primary accession numberP0ACB0
Secondary accession number(s) P03005
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Replicative DNA helicase; EC=3.6.4.12;.
Gene nameName=dnaB
Synonyms=groP, grpA
OrderedLocusNames=b4052, JW4012
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: HOMOHEXAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.94; Mw=49448

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB047.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0ACB0; DNAB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDNAB_ECOLI
Primary accession numberP0ACB0
Secondary accession number(s) P03005 Q2M6Q2
Sequence was last modified on August 13, 1987 (version 1)
Annotations were last modified on October 19, 2011 (version 56)
Name and origin of the protein
DescriptionRecName: Full=Replicative DNA helicase; EC=3.6.4.12;
Gene nameName=dnaB
Synonyms=groP, grpA
OrderedLocusNames=b4052, JW4012
Encoded onName=dnaB; Synonyms=groP, grpA; OrderedLocusNames=b4052, JW4012
Keywords3D-structure; ATP-binding; Complete proteome; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Primosome; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK01174; AAA23689.1; -; Genomic_DNA
EMBLL02312; AAA23690.1; -; Genomic_DNA
EMBLU00006; AAC43146.1; -; Genomic_DNA
EMBLU00096; AAC77022.1; -; Genomic_DNA
EMBLAP009048; BAE78054.1; -; Genomic_DNA
PIRC65213; IQECDB; .
RefSeqNP_418476.1; NC_000913.2; .
PDB1B79; X-ray; 2.30 A; A/B/C/D=16-129
PDB1JWE; NMR; -; A=25-137
PDBsum1B79; -; .
PDBsum1JWE; -; .
ProteinModelPortalP0ACB0; -; .
SMRP0ACB0; 26-463; .
DIPDIP-35913N; -; .
IntActP0ACB0; 29; .
MINTMINT-584605; -; .
SWISS-2DPAGEP0ACB0; -; .
ECO2DBASEB047.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002743; EBESCP00000002743; EBESCG00000002234; .
EnsemblBacteriaEBESCT00000016057; EBESCP00000015348; EBESCG00000015117; .
GeneID948555; -; .
GenomeReviewsAP009048_GR; JW4012; .
GenomeReviewsU00096_GR; b4052; .
KEGGecj:JW4012; -; .
KEGGeco:b4052; -; .
EchoBASEEB0232; -; .
EcoGeneEG10236; dnaB; .
eggNOGCOG0305; -; .
GeneTreeEBGT00050000008962; -; .
HOGENOMHBG734566; -; .
OMAVASDFYS; -; .
ProtClustDBPRK08006; -; .
BioCycEcoCyc:EG10236-MONOMER; -; .
BioCycMetaCyc:EG10236-MONOMER; -; .
GenevestigatorP0ACB0; -; .
GOGO:0005658; C:alpha DNA polymerase:primase complex; IEA:UniProtKB-KW; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0003678; F:DNA helicase activity; IEA:InterPro; .
GOGO:0006269; P:DNA replication; synthesis of RNA primer; IEA:UniProtKB-KW
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR007692; DNA_helicase_DnaB; .
InterProIPR007694; DNA_helicase_DnaB-like_C; .
InterProIPR007693; DNA_helicase_DnaB-like_N; .
InterProIPR016136; DNA_helicase_N/primase_C; .
Gene3DG3DSA:1.10.860.10; DnaB_N; 1; .
PfamPF00772; DnaB; 1; .
PfamPF03796; DnaB_C; 1; .
SMARTSM00382; AAA; 1; .
SUPFAMSSF48024; DNA_helicase_DnaB-like_N; 1; .
TIGRFAMsTIGR00665; DnaB; 1; .
PROSITEPS51199; SF4_HELICASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server