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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DUT_ECOLI




SWISS-2DPAGE:  DUT_ECOLI


DUT_ECOLI


General information about the entry
View entry in simple text format
Entry nameDUT_ECOLI
Primary accession numberP06968
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase; Short=dUTPase; EC=3.6.1.23; AltName: Full=dUTP pyrophosphatase;.
Gene nameName=dut
Synonyms=dnaS, sof
OrderedLocusNames=b3640, JW3615
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMOTRIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.03; Mw=16155

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.10; Mw=17526  [identification data]

MAPPING (identification):
Tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEC017.2; 6TH EDITION.
UniProtKB/Swiss-ProtP06968; DUT_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDUT_ECOLI
Primary accession numberP06968
Secondary accession number(s) Q2M7V4
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 114)
Name and origin of the protein
DescriptionRecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase; Short=dUTPase; EC=3.6.1.23; AltName: Full=dUTP pyrophosphatase;
Gene nameName=dut
Synonyms=dnaS, sof
OrderedLocusNames=b3640, JW3615
Encoded onName=dut; Synonyms=dnaS, sof; OrderedLocusNames=b3640, JW3615
Keywords3D-structure; Complete proteome; Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX01714; CAA25859.1; -; Genomic_DNA
EMBLV01578; CAA24897.1; -; Genomic_DNA
EMBLL10328; AAA61993.1; -; Genomic_DNA
EMBLU00096; AAC76664.1; -; Genomic_DNA
EMBLAP009048; BAE77652.1; -; Genomic_DNA
PIRA30388; WPECDU; .
RefSeqNP_418097.1; NC_000913.2; .
PDB1DUD; X-ray; 2.30 A; A=1-151
PDB1DUP; X-ray; 1.90 A; A=1-151
PDB1EU5; X-ray; 1.45 A; A=1-151
PDB1EUW; X-ray; 1.05 A; A=1-151
PDB1RN8; X-ray; 1.93 A; A=1-151
PDB1RNJ; X-ray; 1.70 A; A=1-151
PDB1SEH; X-ray; 1.47 A; A=1-151
PDB1SYL; X-ray; 1.95 A; A=1-151
PDB2HR6; X-ray; 1.84 A; A=1-151
PDB2HRM; X-ray; 1.70 A; A=1-151
PDBsum1DUD; -; .
PDBsum1DUP; -; .
PDBsum1EU5; -; .
PDBsum1EUW; -; .
PDBsum1RN8; -; .
PDBsum1RNJ; -; .
PDBsum1SEH; -; .
PDBsum1SYL; -; .
PDBsum2HR6; -; .
PDBsum2HRM; -; .
ProteinModelPortalP06968; -; .
SMRP06968; 1-135; .
DisProtDP00337; -; .
DIPDIP-9483N; -; .
IntActP06968; 10; .
MINTMINT-1279301; -; .
SWISS-2DPAGEP06968; -; .
ECO2DBASEC017.2; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002984; EBESCP00000002984; EBESCG00000002444; .
EnsemblBacteriaEBESCT00000016134; EBESCP00000015425; EBESCG00000015194; .
GeneID948607; -; .
GenomeReviewsAP009048_GR; JW3615; .
GenomeReviewsU00096_GR; b3640; .
KEGGecj:JW3615; -; .
KEGGeco:b3640; -; .
EchoBASEEB0247; -; .
EcoGeneEG10251; dut; .
eggNOGCOG0756; -; .
GeneTreeEBGT00050000011819; -; .
HOGENOMHBG436079; -; .
OMALDLRACI; -; .
ProtClustDBPRK00601; -; .
BioCycEcoCyc:DUTP-PYROP-MONOMER; -; .
BioCycMetaCyc:DUTP-PYROP-MONOMER; -; .
GenevestigatorP06968; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0004170; F:dUTP diphosphatase activity; IDA:EcoCyc; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0046080; P:dUTP metabolic process; IEA:InterPro; .
HAMAPMF_00116; dUTPase_bact; 1; -
InterProIPR008180; dUTP_pyroPase; .
InterProIPR008181; dUTP_pyroPase_sf; .
PANTHERPTHR11241; PTHR11241; 1; .
PfamPF00692; dUTPase; 1; .
TIGRFAMsTIGR00576; Dut; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server