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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ENO_ECOLI




SWISS-2DPAGE:  ENO_ECOLI


ENO_ECOLI


General information about the entry
View entry in simple text format
Entry nameENO_ECOLI
Primary accession numberP0A6P9
Secondary accession number(s) P08324
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 6)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase;.
Gene nameName=eno
OrderedLocusNames=b2779, JW2750
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[4]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[5]   PROTEIN EXPRESSION
MEDLINE=83160793; PubMed=6339476; [NCBI, ExPASy, EBI, Israel, Japan]
Smith M.W., Neidhardt F.C.
''''''Proteins induced by anaerobiosis in Escherichia coli'';'';''
J. Bacteriol. 154(1):336-343(1983)
[6]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[7]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.34; Mw=46601  [identification data]
pI=5.29; Mw=46325  [identification data]

EXPRESSION:
INCREASED DURING ANAEROBIC GROWTH [5].

MAPPING (identification):
SPOT 2D-000KV0: TAGGING ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3];
SPOT 2D-000KVF: AMINO ACID COMPOSITION AND SEQUENCE TAG (SKIV) [4].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=4.74; Mw=22910  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.46; Mw=45494  [identification data]
pI=5.20; Mw=40139  [identification data]
pI=5.16; Mw=31562  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6];
SPOT 2D-0014Y3: MICROSEQUENCING (XKIV) [6].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.29; Mw=51580  [identification data]
pI=5.27; Mw=51301  [identification data]
pI=5.16; Mw=51319  [identification data]
pI=5.00; Mw=50698  [identification data]
pI=5.02; Mw=50647  [identification data]
pI=5.26; Mw=50000  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6].



ECOLI6-11 {Escherichia coli(6-11)}
Escherichia coli
ECOLI6-11
  map experimental info
  protein estimated location
 
ECOLI6-11

MAP LOCATIONS:
pI=6.95; Mw=24005  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6].



ECOLI6-9 {Escherichia coli(6-9)}
Escherichia coli
ECOLI6-9
  map experimental info
  protein estimated location
 
ECOLI6-9

MAP LOCATIONS:
pI=6.72; Mw=25314

MAPPING (identification):
GEL MATCHING WITH ECOLI6-11 [6].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.35; Mw=43401  [identification data]

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [7].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A6P9; ENO_ECOLI.
ECO2DBASEF043.8; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6P9; ENO_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameENO_ECOLI
Primary accession numberP0A6P9
Secondary accession number(s) P08324 Q2MA53
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 70)
Name and origin of the protein
DescriptionRecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase;
Gene nameName=eno
OrderedLocusNames=b2779, JW2750
Encoded onName=eno; OrderedLocusNames=b2779, JW2750
Keywords3D-structure; Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome; Secreted.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX82400; CAA57795.1; -; Genomic_DNA
EMBLU29580; AAA69289.1; -; Genomic_DNA
EMBLU00096; AAC75821.1; -; Genomic_DNA
EMBLAP009048; BAE76853.1; -; Genomic_DNA
EMBLM12843; AAA24486.1; -; mRNA
PIRG65059; NOEC; .
RefSeqNP_417259.1; NC_000913.2; .
PDB1E9I; X-ray; 2.48 A; A/B/C/D=2-432
PDB2FYM; X-ray; 1.60 A; A/C/D/F=2-432
PDB3H8A; X-ray; 1.90 A; A/B/C/D=1-432
PDBsum1E9I; -; .
PDBsum2FYM; -; .
PDBsum3H8A; -; .
ProteinModelPortalP0A6P9; -; .
SMRP0A6P9; 2-431; .
DIPDIP-31847N; -; .
IntActP0A6P9; 30; .
MINTMINT-1230935; -; .
PhosSiteP0A6P9; -; .
SWISS-2DPAGEP0A6P9; -; .
2DBase-EcoliP0A6P9; -; .
ECO2DBASEF043.8; 6TH EDITION; .
PRIDEP0A6P9; -; .
EnsemblBacteriaEBESCT00000003599; EBESCP00000003599; EBESCG00000002939; .
EnsemblBacteriaEBESCT00000003600; EBESCP00000003600; EBESCG00000002939; .
EnsemblBacteriaEBESCT00000003601; EBESCP00000003601; EBESCG00000002939; .
EnsemblBacteriaEBESCT00000003602; EBESCP00000003602; EBESCG00000002939; .
EnsemblBacteriaEBESCT00000003603; EBESCP00000003603; EBESCG00000002939; .
EnsemblBacteriaEBESCT00000017301; EBESCP00000016592; EBESCG00000016358; .
GeneID945032; -; .
GenomeReviewsAP009048_GR; JW2750; .
GenomeReviewsU00096_GR; b2779; .
KEGGecj:JW2750; -; .
KEGGeco:b2779; -; .
EchoBASEEB0254; -; .
EcoGeneEG10258; eno; .
eggNOGCOG0148; -; .
GeneTreeEBGT00050000010838; -; .
HOGENOMHBG726599; -; .
OMAQEYMIMP; -; .
ProtClustDBPRK00077; -; .
BioCycEcoCyc:ENOLASE-MONOMER; -; .
BioCycMetaCyc:ENOLASE-MONOMER; -; .
BRENDA4.2.1.11; 2026; .
GenevestigatorP0A6P9; -; .
GOGO:0009986; C:cell surface; IEA:UniProtKB-SubCell; .
GOGO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell; .
GOGO:0005576; C:extracellular region; IEA:UniProtKB-SubCell; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0000287; F:magnesium ion binding; IDA:EcoliWiki; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IDA:EcoliWiki; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006096; P:glycolysis; IMP:EcoliWiki; .
HAMAPMF_00318; Enolase; 1; -
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server