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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: FABI_ECOLI




SWISS-2DPAGE: FABI_ECOLI



FABI_ECOLI:

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ID   FABI_ECOLI; STANDARD; 2DG.
AC   P0AEK4; P29132;
DT   01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 8.
DE   RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] FabI;
DE   Short=ENR; EC=1.3.1.9; AltName: Full=NADH-dependent enoyl-ACP reductase;.
GN   Name=fabI; Synonyms=envM; OrderedLocusNames=b1288, JW1281;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LBQ=5.37/38366;
2D   -!-   PI/MW: SPOT 2D-001LCK=5.33/37266;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LBQ: 997.596; 1295.619; 1311.587;
2D         1327.597; 1430.782; 1446.743; 1485.682; 1655.827; 1993.03; 2009.011;
2D         2797.251; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LCK: 997.838; 1447.162; 1486.1;
2D         1656.257; 2009.576; 2797.999; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WQG=5.40/32959;
2D   -!-   PI/MW: SPOT 2D-001WQS=5.60/32613;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WQS: 859.43; 887.48; 997.53; 1043.6;
2D         1430.75; 1485.67; 1553.8; 1639.8; 1726.87; 1993.1; 2797.44; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WQG: (R)ILVTGVASK(L),8-16;
2D         (R)EGAELAFTYQNDKLK(G),30-44; (R)VNAISAGPIR(T),183-192.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WQS:
2D         (K)FDGFVHSIGFAPGDQLDGDYVNAVTR,84-109; (R)VNAISAGPIR(T),183-192.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [2].
2D   -!-   MAPPING: Tandem mass spectrometry [2]; SPOT 2D-001WQS: PEPTIDE MASS
2D         FINGERPRINTING [2].
CC   ---------------------------------------------------------------------------
CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
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CC   ---------------------------------------------------------------------------
DR   UniProtKB/Swiss-Prot; P0AEK4; FABI_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server