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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: FABI_ECOLI |
SWISS-2DPAGE: FABI_ECOLI
FABI_ECOLI:
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ID FABI_ECOLI; STANDARD; 2DG.
AC P0AEK4; P29132;
DT 01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT 15-MAY-2003, 2D annotation version 2.
DT 19-MAY-2011, general annotation version 8.
DE RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH] FabI;
DE Short=ENR; EC=1.3.1.9; AltName: Full=NADH-dependent enoyl-ACP reductase;.
GN Name=fabI; Synonyms=envM; OrderedLocusNames=b1288, JW1281;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [2]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LBQ=5.37/38366;
2D -!- PI/MW: SPOT 2D-001LCK=5.33/37266;
2D -!- PEPTIDE MASSES: SPOT 2D-001LBQ: 997.596; 1295.619; 1311.587;
2D 1327.597; 1430.782; 1446.743; 1485.682; 1655.827; 1993.03; 2009.011;
2D 2797.251; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001LCK: 997.838; 1447.162; 1486.1;
2D 1656.257; 2009.576; 2797.999; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [1].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WQG=5.40/32959;
2D -!- PI/MW: SPOT 2D-001WQS=5.60/32613;
2D -!- PEPTIDE MASSES: SPOT 2D-001WQS: 859.43; 887.48; 997.53; 1043.6;
2D 1430.75; 1485.67; 1553.8; 1639.8; 1726.87; 1993.1; 2797.44; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WQG: (R)ILVTGVASK(L),8-16;
2D (R)EGAELAFTYQNDKLK(G),30-44; (R)VNAISAGPIR(T),183-192.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WQS:
2D (K)FDGFVHSIGFAPGDQLDGDYVNAVTR,84-109; (R)VNAISAGPIR(T),183-192.
2D -!- EXPRESSION: decrease after benzoic acid treatment [2].
2D -!- MAPPING: Tandem mass spectrometry [2]; SPOT 2D-001WQS: PEPTIDE MASS
2D FINGERPRINTING [2].
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DR UniProtKB/Swiss-Prot; P0AEK4; FABI_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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