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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: GLTD_ECOLI |
SWISS-2DPAGE: GLTD_ECOLI
GLTD_ECOLI:
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ID GLTD_ECOLI; STANDARD; 2DG.
AC P09832;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 01-OCT-2001, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Glutamate synthase [NADPH] small chain; EC=1.4.1.13;
DE AltName: Full=Glutamate synthase subunit beta; Short=GLTS beta chain;
DE AltName: Full=NADPH-GOGAT;.
GN Name=gltD; Synonyms=aspB; OrderedLocusNames=b3213, JW3180;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5-6.
IM ECOLI, ECOLI5-6.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=83270974; PubMed=6308410; [NCBI, Expasy, EBI, Israel, Japan]
RA Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL Microbiol. Rev. 47(1):231-284(1983).
RN [3]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [4]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=92138602; PubMed=1346534; [NCBI, Expasy, EBI, Israel, Japan]
RA Ernsting B.R., Atkinson M.R., Ninfa A.J., Matthews R.G.;
RT ''''''Characterization of the regulon controlled by the
RT leucine-responsive regulatory protein in Escherichia coli'';'';'';
RL J. Bacteriol. 174(1):1109-1118(1992).
RN [6]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
CC -!- SUBUNIT: AGGREGATE OF 4 CATALYTICAL ACTIVE HETERODIMERS
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KQL=5.40/52658;
2D -!- PI/MW: SPOT 2D-000KQS=5.47/52555;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000KQL: B=11.2, Z=12, S=5.2, H=1,
2D G=13.2, T=4.4, A=9.7, P=4.9, Y=1.3, R=7.4, V=7.7, M=2.2, I=4.9, L=6.9,
2D F=4.9, K=3.1;
2D -!- EXPRESSION: AN APPROXIMATELY TWO-FOLD-LOWER GLUTAMATE SYNTHASE
2D ACTIVITY WAS DETECTED WHEN LRP+ STRAINS WERE GROWN IN THE PRESENCE OF
2D EXOGENOUS LEUCINE [5].
2D -!- MAPPING: SPOT 2D-000KQL: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D (SQNV) [4]; SPOT 2D-000KQS: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS
2D BY NEIDHARDT [2] AND VANBOGELEN [3].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001KYZ=5.48/53050;
2D -!- PEPTIDE MASSES: SPOT 2D-001KYZ: 906.441; 947.543; 977.467;
2D 1038.516; 1054.509; 1127.56; 1206.539; 1271.66; 1287.698; 1510.78;
2D 1564.75; 1610.753; 1626.757; 1642.754; 1767.996; 1788.879; 1825.007;
2D 1953.141; 2014.014; 2272.117; 2455.135; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [6].
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DR ECO2DBASE; F050.4; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P09832; GLTD_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(5-6)
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SWISS-2DPAGE (search AC)
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