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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: GLYA_ECOLI |
SWISS-2DPAGE: GLYA_ECOLI
GLYA_ECOLI:
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ID GLYA_ECOLI; STANDARD; 2DG.
AC P0A825; P00477;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Serine hydroxymethyltransferase; Short=SHMT; Short=Serine
DE methylase; EC=2.1.2.1;.
GN Name=glyA; OrderedLocusNames=b2551, JW2535;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: HOMOTETRAMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KVN=5.94/46234;
2D -!- PI/MW: SPOT 2D-000KVU=6.04/46051;
2D -!- MAPPING: SPOT 2D-000KVN: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D (MLK) [3]; SPOT 2D-000KVU: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON
2D 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WKH=6.02/45274;
2D -!- PI/MW: SPOT 2D-001WKJ=6.00/45000;
2D -!- PI/MW: SPOT 2D-001WLG=6.00/43217;
2D -!- PEPTIDE MASSES: SPOT 2D-001WKH: 846.45; 963.47; 1040.44; 1109.49;
2D 1320.58; 1478.7; 1814.91; 2065.94; 2163.01; 2480.19; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WKJ: 870.43; 882.36; 963.46; 1010.45;
2D 1040.43; 1093.49; 1927.84; 2015.8; 2162.82; 2318.94; 2441.07; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WKH: (K)GGSEELYK(K),243-250;
2D (K)TYQQQVAK(N),286-293; (K)NSVPNDPKSPFVTSGIR(V),347-363.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WKJ:
2D (K)LYNIVPYGIDATGHIDYADLEK(Q),139-160; (K)GGSEELYK(S),243-250;
2D (K)VVSGGTDNHLFLVDLVDK(N),309-326; (K)NSVPNDPK(S),347-354.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WLG: (K)TYQQQVAK(N),286-293; (K)NSVPNDPKSPFVTSGIR(V),347-363.
2D -!- EXPRESSION: increase after benzoic acid treatment [4].
2D -!- MAPPING: SPOTS 1WK*: PEPTIDE MASS FINGERPRINTING [4]; Tandem mass
2D spectrometry [4].
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DR 2DBase-Ecoli; P0A825; GLYA_ECOLI.
DR ECO2DBASE; G043.8; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0A825; GLYA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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