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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GLYC_MOUSE




SWISS-2DPAGE:  GLYC_MOUSE


GLYC_MOUSE


General information about the entry
View entry in simple text format
Entry nameGLYC_MOUSE
Primary accession numberP50431
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Serine hydroxymethyltransferase, cytosolic; Short=SHMT; EC=2.1.2.1; AltName: Full=Glycine hydroxymethyltransferase; AltName: Full=Serine methylase;.
Gene nameName=Shmt1
Synonyms=Shmt
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
MEDLINE=93162044; PubMed=1286668; [NCBI, ExPASy, EBI, Israel, Japan]
Giometti C.S., Taylor J., Tollaksen S.L.
''''''Mouse liver protein database: a catalog of proteins detected by two- dimensional gel electrophoresis'';'';''
Electrophoresis 13(1):970-991(1992)
[3]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=6.75; Mw=35104

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [3].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.78; Mw=32004

MAPPING (identification):
SPOT 2D-00140Z: MATCHING [3] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1], AND WITH GIOMETTI MOUSE LIVER 2-DE MAP [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=6.71; Mw=35503

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
REPRODUCTION-2DPAGEP50431; P50431.
UniProtKB/Swiss-ProtP50431; GLYC_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGLYC_MOUSE
Primary accession numberP50431
Secondary accession number(s) Q64508 Q8R0X9
Sequence was last modified on July 27, 2011 (version 3)
Annotations were last modified on October 19, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=Serine hydroxymethyltransferase, cytosolic; Short=SHMT; EC=2.1.2.1; AltName: Full=Glycine hydroxymethyltransferase; AltName: Full=Serine methylase;
Gene nameName=Shmt1
Synonyms=Shmt
Encoded onName=Shmt1; Synonyms=Shmt
Keywords3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; One-carbon metabolism; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF237702; AAK15040.1; -; mRNA
EMBLAL596215; CAI35264.1; -; Genomic_DNA
EMBLBC026055; AAH26055.1; -; mRNA
EMBLX94478; CAA64225.1; -; mRNA
EMBLX94479; CAA64226.1; -; mRNA
IPIIPI00118059; -; .
PIRJC4959; JC4959; .
RefSeqNP_033197.2; NM_009171.2; .
UniGeneMm.364956; -; .
PDB1EJI; X-ray; 2.90 A; A/B/C/D=1-478
PDBsum1EJI; -; .
ProteinModelPortalP50431; -; .
STRINGP50431; -; .
PhosphoSiteP50431; -; .
SWISS-2DPAGEP50431; -; .
REPRODUCTION-2DPAGEP50431; -; .
PRIDEP50431; -; .
EnsemblENSMUST00000018744; ENSMUSP00000018744; ENSMUSG00000020534; .
GeneID20425; -; .
NMPDRfig|10090.3.peg.23855; -; .
MGIMGI:98299; Shmt1; .
eggNOGroNOG08367; -; .
HOGENOMHBG301263; -; .
HOVERGENHBG002807; -; .
InParanoidP50431; -; .
OrthoDBEOG42Z4Q2; -; .
PhylomeDBP50431; -; .
ArrayExpressP50431; -; .
BgeeP50431; -; .
CleanExMM_SHMT1; -; .
GenevestigatorP50431; -; .
GermOnlineENSMUSG00000020534; Mus musculus; .
GOGO:0005739; C:mitochondrion; IDA:MGI; .
GOGO:0004372; F:glycine hydroxymethyltransferase activity; IEA:EC; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0006544; P:glycine metabolic process; IEA:InterPro; .
GOGO:0006563; P:L-serine metabolic process; IEA:InterPro; .
GOGO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW; .
InterProIPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterProIPR015421; PyrdxlP-dep_Trfase_major_sub1; .
InterProIPR015422; PyrdxlP-dep_Trfase_major_sub2; .
InterProIPR001085; Ser_HO-MeTrfase; .
InterProIPR019798; Ser_HO-MeTrfase_PLP_BS; .
Gene3DG3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
Gene3DG3DSA:3.90.1150.10; PyrdxlP-dep_Trfase_major_sub2; 1; .
PANTHERPTHR11680; Gly_HO-Metrfase; 1; .
PfamPF00464; SHMT; 1; .
PIRSFPIRSF000412; SHMT; 1; .
SUPFAMSSF53383; PyrdxlP-dep_Trfase_major; 1; .
PROSITEPS00096; SHMT; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server