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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GRCA_ECOLI




SWISS-2DPAGE:  GRCA_ECOLI


GRCA_ECOLI


General information about the entry
View entry in simple text format
Entry nameGRCA_ECOLI
Primary accession numberP68066
Secondary accession number(s) P33633
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onOctober 1, 2001 (version 4)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Autonomous glycyl radical cofactor;.
Gene nameName=grcA
Synonyms=yfiD
OrderedLocusNames=b2579, JW2563
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.01; Mw=15083  [identification data]
pI=5.00; Mw=12496
pI=5.13; Mw=11775  [identification data]

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (MITG) [1].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=5.01; Mw=11128  [identification data]
pI=4.96; Mw=10675  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.09; Mw=11594  [identification data]
pI=5.05; Mw=9918
pI=5.23; Mw=9795  [identification data]
pI=5.21; Mw=9583

MAPPING (identification):
SPOTS 15FR, 15H1: Peptide mass fingerprinting [2]; SPOTS 15GW, 15H7: MICROSEQUENCING (MITGI) [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP68066; GRCA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRCA_ECOLI
Primary accession numberP68066
Secondary accession number(s) P33633
Sequence was last modified on December 21, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Autonomous glycyl radical cofactor;
Gene nameName=grcA
Synonyms=yfiD
OrderedLocusNames=b2579, JW2563
Encoded onName=grcA; Synonyms=yfiD; OrderedLocusNames=b2579, JW2563
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Organic radical; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD13169; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLD64044; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLU00096; AAC75632.1; -; Genomic_DNA
EMBLAP009048; BAA16465.1; -; Genomic_DNA
EMBLJ03725; -; NOT_ANNOTATED_CDS; Genomic_DNA
PIRB65036; B65036; .
RefSeqNP_417074.1; NC_000913.2; .
ProteinModelPortalP68066; -; .
SMRP68066; 13-127; .
DIPDIP-47913N; -; .
IntActP68066; 12; .
SWISS-2DPAGEP68066; -; .
EnsemblBacteriaEBESCT00000003623; EBESCP00000003623; EBESCG00000002956; .
EnsemblBacteriaEBESCT00000016113; EBESCP00000015404; EBESCG00000015173; .
GeneID947068; -; .
GenomeReviewsAP009048_GR; JW2563; .
GenomeReviewsU00096_GR; b2579; .
KEGGecj:JW2563; -; .
KEGGeco:b2579; -; .
EchoBASEEB1732; -; .
EcoGeneEG11784; grcA; .
eggNOGCOG3445; -; .
GeneTreeEBGT00050000009047; -; .
HOGENOMHBG297207; -; .
OMAGQFEYRE; -; .
ProtClustDBPRK11127; -; .
BioCycEcoCyc:EG11784-MONOMER; -; .
GenevestigatorP68066; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0003824; F:catalytic activity; IEA:InterPro; .
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
HAMAPMF_00806; GrcA; 1; -
InterProIPR001150; Form_AcTrfase_GR; .
InterProIPR019777; Form_AcTrfase_GR_CS; .
InterProIPR011140; Glycyl_radical_cofactor_YfiD; .
PfamPF01228; Gly_radical; 1; .
PIRSFPIRSF000378; Gly_radicl_yfiD; 1; .
PROSITEPS00850; GLY_RADICAL_1; 1; .
PROSITEPS51149; GLY_RADICAL_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server