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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: GRCA_ECOLI |
SWISS-2DPAGE: GRCA_ECOLI
GRCA_ECOLI:
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ID GRCA_ECOLI; STANDARD; 2DG.
AC P68066; P33633;
DT 01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT 01-OCT-2001, 2D annotation version 4.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Autonomous glycyl radical cofactor;.
GN Name=grcA; Synonyms=yfiD; OrderedLocusNames=b2579, JW2563;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [2]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LSC=5.01/15083;
2D -!- PI/MW: SPOT 2D-000LTS=5.00/12496;
2D -!- PI/MW: SPOT 2D-000LUC=5.13/11775;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000LSC: B=8.6, Z=13.5, S=5.6,
2D H=0.5, G=8.3, T=5.2, A=9.3, P=4.7, Y=2.2, R=7.8, V=11.5, M=0.7, I=5.1,
2D L=9.3, F=2.9, K=4.7;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000LUC: B=6.3, Z=14.8, S=5.1,
2D H=0.3, G=9.1, T=3.6, A=10, P=4.7, Y=1.9, R=6.8, V=13.3, M=1.2, I=5.4,
2D L=11, F=1.5, K=5.1;
2D -!- MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (MITG) [1].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HON=5.01/11128; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001HOT=4.96/10675;
2D -!- PEPTIDE MASSES: SPOT 2D-001HON: 890.475; 1219.58; 1280.686;
2D 1381.727; 1434.77; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001HOT: 865.46; 890.571; 1219.673;
2D 1280.782; 1381.807; 1434.842; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [2].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015FR=5.09/11594;
2D -!- PI/MW: SPOT 2D-0015GW=5.05/9918;
2D -!- PI/MW: SPOT 2D-0015H1=5.23/9795;
2D -!- PI/MW: SPOT 2D-0015H7=5.21/9583;
2D -!- PEPTIDE MASSES: SPOT 2D-0015FR: 751.415; 1219.59; 1280.7; 1381.76;
2D 1434.75; 1590.85; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-0015H1: 890.496; 1280.677; 1434.771; TRYPSIN.
2D -!- MAPPING: SPOTS 15FR, 15H1: Peptide mass fingerprinting [2]; SPOTS
2D 15GW, 15H7: MICROSEQUENCING (MITGI) [2].
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DR UniProtKB/Swiss-Prot; P68066; GRCA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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SWISS-2DPAGE (search AC)
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