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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GRP75_HUMAN




SWISS-2DPAGE: GRP75_HUMAN



GRP75_HUMAN:

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ID   GRP75_HUMAN; STANDARD; 2DG.
AC   P38646; P30036;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 5.
DT   19-MAY-2011, general annotation version 17.
DE   RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa
DE   glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa
DE   protein 9; AltName: Full=Mortalin; Short=MOT; AltName:
DE   Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor;.
GN   Name=HSPA9; Synonyms=GRP75, HSPA9B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HL60_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HL60_HUMAN, KIDNEY_HUMAN, LIVER_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot
RA   J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.;
RT   ''''''Human liver protein map: update 1993'';'';'';
RL   Electrophoresis 14(1):1216-1222(1993).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [4]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [5]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [6]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [7]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
RN   [8]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000T66=5.40/73173;
2D   -!-   PI/MW: SPOT 2D-000T6C=5.32/73173;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL AND MICROSEQUENCING [6].
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001DJI=5.27/71362;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001DJI: 958.08; 1148.077; 1242.261;
2D         1290.268; 1361.326; 1450.365; 1462.352; 1592.513; 1694.443; 2055.579; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [7].
2D   -!- MASTER: ELC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000EPC=5.33/24594; !FRAGMENT!
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000YI8=5.28/67250;
2D   -!-   PI/MW: SPOT 2D-000YIG=5.23/67250;
2D   -!-   PI/MW: SPOT 2D-000YIK=5.18/67055;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [4][5].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000MPF=5.27/66791;
2D   -!-   PI/MW: SPOT 2D-000MPG=5.30/66571;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000MPG: B=12, Z=17.1, S=8.3, H=0.9,
2D         G=16.3, T=3.8, A=14.1, P=3.6, Y=4.3, R=4.1, V=4.2, M=1.9, I=1.5, L=4.2,
2D         F=2.9, K=0.7;
2D   -!-   MAPPING: MICROSEQUENCING AND AMINO ACID COMPOSITION ANALYSIS [3].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0001CF=5.30/25652; !FRAGMENT!
2D   -!-   MAPPING: MICROSEQUENCING (ASEAIKGAV) [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VGG=5.69/72414;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VGG: 1045.5; 1149.5; 1242.7; 1290.6;
2D         1361.7; 1450.7; 1462.7; 1569.8; 1592.9; 1694.8; 1808.8; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [8].
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DR   DOSAC-COBS 2D-PAGE; P38646; P38646.
DR   DOSAC-COBS-2DPAGE; P38646; GRP75_HUMAN.
DR   HSC-2DPAGE; P38646; HUMAN.
DR   OGP-WWW; P38646.
DR   PHCI-2DPAGE; P38646; GR75_HUMAN.
DR   REPRODUCTION-2DPAGE; IPI00007765; IPI00007765.
DR   Siena-2DPAGE; P38646; GRP75_HUMAN.
DR   UCD-2DPAGE; P38646; GRP75_HUMAN.
DR   UniProtKB/Swiss-Prot; P38646; GRP75_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal epithelia cells
CEC_HUMAN
Colorectal adenocarcinoma cell line (DL-1)
DLD1_HUMAN
Erythroleukemia Cell
ELC_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Liver
LIVER_HUMAN
2D-PAGE of nucleolar proteins from Human HeLa cells
NUCLEOLI_HELA_2D_HUMAN
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server