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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: GRPE_ECOLI




SWISS-2DPAGE:  GRPE_ECOLI


GRPE_ECOLI


General information about the entry
View entry in simple text format
Entry nameGRPE_ECOLI
Primary accession numberP09372
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Protein grpE; AltName: Full=HSP-70 cofactor; AltName: Full=HSP24; AltName: Full=Heat shock protein B25.3;.
Gene nameName=grpE
OrderedLocusNames=b2614, JW2594
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[4]   PROTEIN EXPRESSION
MEDLINE=87083376; PubMed=3539918; [NCBI, ExPASy, EBI, Israel, Japan]
Vanbogelen R.A., Kelley P.M., Neidhardt F.C.
''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';''
J. Bacteriol. 169(1):26-32(1987)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.68; Mw=25564

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEB025.3; 6TH EDITION.
UniProtKB/Swiss-ProtP09372; GRPE_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRPE_ECOLI
Primary accession numberP09372
Sequence was last modified on July 1, 1989 (version 1)
Annotations were last modified on October 19, 2011 (version 108)
Name and origin of the protein
DescriptionRecName: Full=Protein grpE; AltName: Full=HSP-70 cofactor; AltName: Full=HSP24; AltName: Full=Heat shock protein B25.3;
Gene nameName=grpE
OrderedLocusNames=b2614, JW2594
Encoded onName=grpE; OrderedLocusNames=b2614, JW2594
Keywords3D-structure; Chaperone; Complete proteome; Cytoplasm; Reference proteome; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX07863; CAA30711.1; -; Genomic_DNA
EMBLU00096; AAC75663.1; -; Genomic_DNA
EMBLAP009048; BAA16498.1; -; Genomic_DNA
PIRS01240; S01240; .
RefSeqNP_417104.1; NC_000913.2; .
PDB1DKG; X-ray; 2.80 A; A/B=1-197
PDBsum1DKG; -; .
ProteinModelPortalP09372; -; .
SMRP09372; 34-197; .
DisProtDP00103; -; .
DIPDIP-6141N; -; .
IntActP09372; 49; .
MINTMINT-1225400; -; .
SWISS-2DPAGEP09372; -; .
ECO2DBASEB025.3; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001332; EBESCP00000001332; EBESCG00000001105; .
EnsemblBacteriaEBESCT00000016304; EBESCP00000015595; EBESCG00000015364; .
GeneID947097; -; .
GenomeReviewsAP009048_GR; JW2594; .
GenomeReviewsU00096_GR; b2614; .
KEGGecj:JW2594; -; .
KEGGeco:b2614; -; .
EchoBASEEB0411; -; .
EcoGeneEG10416; grpE; .
eggNOGCOG0576; -; .
GeneTreeEBGT00050000009835; -; .
HOGENOMHBG732630; -; .
OMANVPFNPE; -; .
ProtClustDBPRK10325; -; .
BioCycEcoCyc:EG10416-MONOMER; -; .
GenevestigatorP09372; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:InterPro; .
GOGO:0051087; F:chaperone binding; IEA:InterPro; .
GOGO:0042803; F:protein homodimerization activity; IEA:InterPro; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
GOGO:0043335; P:protein unfolding; IDA:EcoCyc; .
GOGO:0009408; P:response to heat; IEP:EcoliWiki; .
HAMAPMF_01151; GrpE; 1; -
InterProIPR000740; GrpE; .
InterProIPR013805; GrpE_coiled_coil; .
InterProIPR009012; GrpE_head; .
Gene3DG3DSA:3.90.20.20; GrpE_coiled_coil; 1; .
Gene3DG3DSA:2.30.22.10; GrpE_head; 1; .
PANTHERPTHR21237; GrpE; 1; .
PfamPF01025; GrpE; 1; .
PRINTSPR00773; GRPEPROTEIN; .
SUPFAMSSF51064; GrpE_head; 1; .
PROSITEPS01071; GRPE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server