resource logo

SWISS-2DPAGE

Attention: World-2DPAGE is no longer maintained.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: HSP77_YEAST




SWISS-2DPAGE:  HSP77_YEAST


HSP77_YEAST


General information about the entry
View entry in simple text format
Entry nameHSP77_YEAST
Primary accession numberP12398
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Heat shock protein SSC1, mitochondrial; AltName: Full=Endonuclease SceI 75 kDa subunit; Short=Endo.SceI 75 kDa subunit; AltName: Full=mtHSP70; Flags: Precursor;.
Gene nameName=SSC1
Synonyms=ENS1
OrderedLocusNames=YJR045C
ORFNames=J1639
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=91171929; PubMed=2005818; [NCBI, Expasy, EBI, Israel, Japan]
Nicolet C.M., Craig E.A.
''''''Inducing and assaying heat-shock response in Saccharomyces cerevisiae'';'';''
Meth. Enzymol. 194(1):710-717(1991)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=5.12; Mw=74241
pI=5.17; Mw=74033

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2D GELS BY NICOLET AND CRAIG [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UCD-2DPAGEP12398; HSP77_YEAST.
UniProtKB/Swiss-ProtP12398; HSP77_YEAST.
YEPD8692.
YEPD8704.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHSP77_YEAST
Primary accession numberP12398
Secondary accession number(s) D6VWL6
Sequence was last modified on October 1, 1989 (version 1)
Annotations were last modified on October 19, 2011 (version 129)
Name and origin of the protein
DescriptionRecName: Full=Heat shock protein SSC1, mitochondrial; AltName: Full=Endonuclease SceI 75 kDa subunit; Short=Endo.SceI 75 kDa subunit; AltName: Full=mtHSP70; Flags: Precursor;
Gene nameName=SSC1
Synonyms=ENS1
OrderedLocusNames=YJR045C
ORFNames=J1639
Encoded onName=SSC1; Synonyms=ENS1; OrderedLocusNames=YJR045C; ORFNames=J1639
KeywordsATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Stress response; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM27229; AAA63792.1; -; Genomic_DNA
EMBLM55275; AAA34590.1; -; Genomic_DNA
EMBLL36344; AAA88747.1; -; Genomic_DNA
EMBLZ49545; CAA89573.1; -; Genomic_DNA
EMBLBK006943; DAA08832.1; -; Genomic_DNA
PIRA32493; HHBYS1; .
RefSeqNP_012579.1; NM_001181703.1; .
ProteinModelPortalP12398; -; .
SMRP12398; 29-638; .
DIPDIP-411N; -; .
IntActP12398; 35; .
MINTMINT-665405; -; .
STRINGP12398; -; .
SWISS-2DPAGEP12398; -; .
UCD-2DPAGEP12398; -; .
PeptideAtlasP12398; -; .
EnsemblFungiYJR045C; YJR045C; YJR045C; .
GeneID853503; -; .
KEGGsce:YJR045C; -; .
NMPDRfig|4932.3.peg.3553; -; .
CYGDYJR045c; -; .
SGDS000003806; SSC1; .
eggNOGfuNOG06906; -; .
GeneTreeEFGT00050000000911; -; .
HOGENOMHBG334976; -; .
OMAQSMTIAS; -; .
OrthoDBEOG4FV07S; -; .
PhylomeDBP12398; -; .
NextBio974151; -; .
ArrayExpressP12398; -; .
GenevestigatorP12398; -; .
GermOnlineYJR045C; Saccharomyces cerevisiae; .
GOGO:0042645; C:mitochondrial nucleoid; IDA:SGD; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0001405; C:presequence translocase-associated import motor; IDA:SGD; .
GOGO:0072380; C:TRC complex; IDA:SGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0016887; F:ATPase activity; IDA:SGD; .
GOGO:0030234; F:enzyme regulator activity; IDA:SGD; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0032079; P:positive regulation of endodeoxyribonuclease activity; IDA:SGD; .
GOGO:0030150; P:protein import into mitochondrial matrix; IDA:SGD; .
GOGO:0042026; P:protein refolding; IDA:SGD; .
GOGO:0043335; P:protein unfolding; IMP:SGD; .
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server