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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: IDH_ECOLI




SWISS-2DPAGE:  IDH_ECOLI


IDH_ECOLI


General information about the entry
View entry in simple text format
Entry nameIDH_ECOLI
Primary accession numberP08200
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase;.
Gene nameName=icd
Synonyms=icdA, icdE
OrderedLocusNames=b1136, JW1122
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=81069671; PubMed=7002901; [NCBI, ExPASy, EBI, Israel, Japan]
Phillips T.A., Bloch P.L., Neidhardt F.C.
''''''Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins'';'';''
J. Bacteriol. 144(1):1024-1033(1980)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.02; Mw=46142

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND PHILLIPS [3].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=4.70; Mw=22389  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.15; Mw=43958  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.00; Mw=74518  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.11; Mw=44335  [identification data]
pI=5.15; Mw=44053  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [5].

MAPPING (identification):
Peptide mass fingerprinting [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP08200; IDH_ECOLI.
ECO2DBASEC043.8; 6TH EDITION.
UniProtKB/Swiss-ProtP08200; IDH_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameIDH_ECOLI
Primary accession numberP08200
Sequence was last modified on August 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 120)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase;
Gene nameName=icd
Synonyms=icdA, icdE
OrderedLocusNames=b1136, JW1122
Encoded onName=icd; Synonyms=icdA, icdE; OrderedLocusNames=b1136, JW1122
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02799; AAA24006.1; -; Genomic_DNA
EMBLU00096; AAC74220.1; -; Genomic_DNA
EMBLAP009048; BAA35958.1; -; Genomic_DNA
EMBLAF017587; AAC45887.1; -; Genomic_DNA
PIRA28482; DCECIS; .
RefSeqNP_415654.1; NC_000913.2; .
PDB1AI2; X-ray; 1.90 A; A=1-416
PDB1AI3; X-ray; 1.90 A; A=1-416
PDB1BL5; X-ray; 2.50 A; A=3-416
PDB1CW1; X-ray; 2.10 A; A=1-416
PDB1CW4; X-ray; 2.10 A; A=1-416
PDB1CW7; X-ray; 2.60 A; A=1-416
PDB1GRO; X-ray; 2.50 A; A=1-416
PDB1GRP; X-ray; 2.50 A; A=1-416
PDB1HJ6; X-ray; 2.00 A; A=1-416
PDB1IDC; X-ray; 2.50 A; A=1-416
PDB1IDD; X-ray; 2.50 A; A=1-416
PDB1IDE; X-ray; 2.50 A; A=1-416
PDB1IDF; X-ray; 2.50 A; A=1-416
PDB1IKA; X-ray; 2.70 A; A=1-416
PDB1ISO; X-ray; 1.90 A; A=1-416
PDB1P8F; X-ray; 1.85 A; A=1-416
PDB1PB1; X-ray; 1.70 A; A=1-416
PDB1PB3; X-ray; 1.70 A; A=1-416
PDB1SJS; X-ray; 2.42 A; A=1-416
PDB3ICD; X-ray; 2.50 A; A=1-416
PDB3LCB; X-ray; 2.90 A; C/D=1-416
PDB4ICD; X-ray; 2.50 A; A=1-416
PDB5ICD; X-ray; 2.50 A; A=1-416
PDB6ICD; X-ray; 2.80 A; A=1-416
PDB7ICD; X-ray; 2.40 A; A=1-416
PDB8ICD; X-ray; 2.50 A; A=1-416
PDB9ICD; X-ray; 2.50 A; A=1-416
PDBsum1AI2; -; .
PDBsum1AI3; -; .
PDBsum1BL5; -; .
PDBsum1CW1; -; .
PDBsum1CW4; -; .
PDBsum1CW7; -; .
PDBsum1GRO; -; .
PDBsum1GRP; -; .
PDBsum1HJ6; -; .
PDBsum1IDC; -; .
PDBsum1IDD; -; .
PDBsum1IDE; -; .
PDBsum1IDF; -; .
PDBsum1IKA; -; .
PDBsum1ISO; -; .
PDBsum1P8F; -; .
PDBsum1PB1; -; .
PDBsum1PB3; -; .
PDBsum1SJS; -; .
PDBsum3ICD; -; .
PDBsum3LCB; -; .
PDBsum4ICD; -; .
PDBsum5ICD; -; .
PDBsum6ICD; -; .
PDBsum7ICD; -; .
PDBsum8ICD; -; .
PDBsum9ICD; -; .
ProteinModelPortalP08200; -; .
SMRP08200; 1-416; .
IntActP08200; 5; .
PhosSiteP08200; -; .
SWISS-2DPAGEP08200; -; .
2DBase-EcoliP08200; -; .
ECO2DBASEC043.8; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000711; EBESCP00000000711; EBESCG00000000593; .
EnsemblBacteriaEBESCT00000000712; EBESCP00000000712; EBESCG00000000593; .
EnsemblBacteriaEBESCT00000015223; EBESCP00000014514; EBESCG00000014283; .
GeneID945702; -; .
GenomeReviewsAP009048_GR; JW1122; .
GenomeReviewsU00096_GR; b1136; .
KEGGecj:JW1122; -; .
KEGGeco:b1136; -; .
EchoBASEEB0484; -; .
EcoGeneEG10489; icd; .
EcoGeneEG10009; icdC; .
eggNOGCOG0538; -; .
GeneTreeEBGT00050000009255; -; .
HOGENOMHBG518924; -; .
OMAFQQIQTR; -; .
ProtClustDBPRK07006; -; .
BioCycEcoCyc:ISOCITDEH-SUBUNIT; -; .
BioCycMetaCyc:ISOCITDEH-SUBUNIT; -; .
GenevestigatorP08200; -; .
GOGO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:EC; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0022900; P:electron transport chain; IMP:EcoliWiki; .
GOGO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:EcoliWiki; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004439; Isocitrate_DH_NADP_dimer_prok; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00183; Prok_nadp_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server