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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: K6PF1_ECOLI




SWISS-2DPAGE: K6PF1_ECOLI



K6PF1_ECOLI:

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ID   K6PF1_ECOLI; STANDARD; 2DG.
AC   P0A796; P06998;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 15.
DE   RecName: Full=6-phosphofructokinase isozyme 1; EC=2.7.1.11; AltName:
DE   Full=6-phosphofructokinase isozyme I; AltName: Full=Phosphofructokinase
DE   1; AltName: Full=Phosphohexokinase 1;.
GN   Name=pfkA; OrderedLocusNames=b3916, JW3887;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.;
RT   ''''''Gene-protein index of Escherichia coli K-12'';'';'';
RL   Microbiol. Rev. 47(1):231-284(1983).
RN   [3]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L3I=5.45/38232;
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT
2D         [2] AND VANBOGELEN [3].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001L85=5.43/45820;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L85: 865.593; 947.599; 994.732;
2D         1084.684; 1100.665; 1641.08; 1657.082; 1700.126; 1716.091; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WOI=5.43/36348;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WOI: 865.47; 949.45; 1100.53; 1715.93; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
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CC   its content is in no way modified and this statement is not removed.
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CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; F035.8; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A796; K6PF1_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server