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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: KAD_ECOLI |
SWISS-2DPAGE: KAD_ECOLI
KAD_ECOLI:
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ID KAD_ECOLI; STANDARD; 2DG.
AC P69441; P05082;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 01-OCT-2001, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 10.
DE RecName: Full=Adenylate kinase; Short=AK; EC=2.7.4.3; AltName:
DE Full=ATP-AMP transphosphorylase;.
GN Name=adk; Synonyms=dnaW, plsA; OrderedLocusNames=b0474, JW0463;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6.
IM ECOLI, ECOLI4-5, ECOLI4.5-5.5, ECOLI5-6.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
CC -!- SUBUNIT: MONOMER
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000LE3=5.49/28491;
2D -!- PI/MW: SPOT 2D-000LKL=5.60/22509;
2D -!- MAPPING: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY
2D VANBOGELEN [2].
2D -!- MASTER: ECOLI4-5;
2D -!- PI/MW: SPOT 2D-001HQ8=5.00/9979; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001HQ8: 834.348; 891.369; 974.458;
2D 1009.599; 1135.588; 1152.559; 1168.575; 1312.816; 1450.769; 1466.749;
2D 2520.331; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-0015GO=4.99/10074; !FRAGMENT!
2D -!- MAPPING: MICROSEQUENCING (MRIIL) [3].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LHC=5.48/31661;
2D -!- PEPTIDE MASSES: SPOT 2D-001LHC: 879.503; 891.421; 974.56; 990.502;
2D 1009.654; 1015.571; 1119.561; 1135.607; 1152.624; 1168.586; 1170.683;
2D 1235.618; 1312.829; 1450.781; 1466.77; 1648.837; 2013.062; 2029.03;
2D 2520.305; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [3].
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DR ECO2DBASE; F026.0; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P69441; KAD_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(4-5)
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Escherichia coli(4.5-5.5)
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Escherichia coli(5-6)
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SWISS-2DPAGE (search AC)
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