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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: KDSB_ECOLI




SWISS-2DPAGE:  KDSB_ECOLI


KDSB_ECOLI


General information about the entry
View entry in simple text format
Entry nameKDSB_ECOLI
Primary accession numberP04951
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase; EC=2.7.7.38; AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase; Short=CKS; Short=CMP-KDO synthase;.
Gene nameName=kdsB
OrderedLocusNames=b0918, JW0901
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.06; Mw=33758

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEC032.7; 6TH EDITION.
UniProtKB/Swiss-ProtP04951; KDSB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameKDSB_ECOLI
Primary accession numberP04951
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 116)
Name and origin of the protein
DescriptionRecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase; EC=2.7.7.38; AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase; Short=CKS; Short=CMP-KDO synthase;
Gene nameName=kdsB
OrderedLocusNames=b0918, JW0901
Encoded onName=kdsB; OrderedLocusNames=b0918, JW0901
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Lipopolysaccharide biosynthesis; Magnesium; Nucleotidyltransferase; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ02614; AAA83877.1; -; Genomic_DNA
EMBLU00096; AAC74004.1; -; Genomic_DNA
EMBLAP009048; BAA35664.1; -; Genomic_DNA
PIRA26322; A26322; .
RefSeqNP_415438.1; NC_000913.2; .
PDB1VH1; X-ray; 2.60 A; A/B/C/D=2-247
PDB3K8D; X-ray; 1.90 A; A/B/C/D=1-248
PDB3K8E; X-ray; 2.51 A; A/B/C/D=1-248
PDBsum1VH1; -; .
PDBsum3K8D; -; .
PDBsum3K8E; -; .
ProteinModelPortalP04951; -; .
SMRP04951; 2-247; .
DIPDIP-10066N; -; .
IntActP04951; 17; .
MINTMINT-1224838; -; .
SWISS-2DPAGEP04951; -; .
ECO2DBASEC032.7; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001713; EBESCP00000001713; EBESCG00000001411; .
EnsemblBacteriaEBESCT00000016199; EBESCP00000015490; EBESCG00000015259; .
GeneID945539; -; .
GenomeReviewsAP009048_GR; JW0901; .
GenomeReviewsU00096_GR; b0918; .
KEGGecj:JW0901; -; .
KEGGeco:b0918; -; .
EchoBASEEB0514; -; .
EcoGeneEG10519; kdsB; .
eggNOGCOG1212; -; .
GeneTreeEBGT00050000011443; -; .
HOGENOMHBG637773; -; .
OMAFSRAPLP; -; .
ProtClustDBPRK05450; -; .
BioCycEcoCyc:CPM-KDOSYNTH-MONOMER; -; .
BioCycMetaCyc:CPM-KDOSYNTH-MONOMER; -; .
GenevestigatorP04951; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0008690; F:3-deoxy-manno-octulosonate cytidylyltransferase activity; IDA:EcoCyc; .
GOGO:0000287; F:magnesium ion binding; IDA:EcoCyc; .
GOGO:0019294; P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IDA:EcoCyc; .
HAMAPMF_00057; CMP-KDO_synth; 1; -
InterProIPR003329; Cytidylyl_trans; .
InterProIPR004528; KdsB; .
PfamPF02348; CTP_transf_3; 1; .
TIGRFAMsTIGR00466; KdsB; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server