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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: KPYM_MOUSE SWISS-2DPAGE:  KPYM_MOUSE KPYM_MOUSE General information about the entry View entry in simple text format Entry name KPYM_MOUSE Primary accession number P52480 integrated into SWISS-2DPAGE on  June 30, 1999 (release 10) 2D Annotations were last modified on May 15, 2003 (version 2) General Annotations were last modified on  May 19, 2011 (version 10) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;. Gene name Name=Pkm2 Synonyms=Pk3, Pykm Annotated species Mus musculus (Mouse) [TaxID: 10090] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus. References [1]   MAPPING ON GEL PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan] Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F. ''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';'' Proteomics 1(1):136-163(2001) 2D PAGE maps for identified proteins How to interpret a protein ISLETS_MOUSE {Pancreatic islet cells} Mus musculus (Mouse) Tissue: Pancreatic islet   map experimental info   protein estimated location   ISLETS_MOUSE MAP LOCATIONS: SPOT 2D-0018FE: pI=7.08; Mw=62739   [identification data] MAPPING (identification): Peptide mass fingerprinting [1]. MUSCLE_MOUSE {Gastrocnemius muscle} Mus musculus (Mouse) Tissue: Gastrocnemius   map experimental info   protein estimated location   MUSCLE_MOUSE MAP LOCATIONS: SPOT 2D-0016S6: pI=6.80; Mw=56422   [identification data] SPOT 2D-0016SD: pI=6.71; Mw=56578   [identification data] MAPPING (identification): Peptide mass fingerprinting [1]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references PMMA-2DPAGE P52480. REPRODUCTION-2DPAGE IPI00225961; IPI00225961. REPRODUCTION-2DPAGE IPI00845840; IPI00845840. REPRODUCTION-2DPAGE IPI00407130; IPI00407130. REPRODUCTION-2DPAGE IPI00116279; IPI00116279. REPRODUCTION-2DPAGE P52480; P52480. UniProtKB/Swiss-Prot P52480; KPYM_MOUSE. World-2DPAGE Repository P52480; KPYM_MOUSE. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name KPYM_MOUSE Primary accession number P52480 Secondary accession number(s) Q3TBV8 Q3TBW5 Q3TC59 Q3U1X3 Q3U5P6 Q4VC20 Q64484 Q91YI8 Q9CWB1 Sequence was last modified on  May 1, 2007 (version 4) Annotations were last modified on  October 19, 2011 (version 125) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme; Gene name Name=Pkm2 Synonyms=Pk3, Pykm Encoded on Name=Pkm2; Synonyms=Pk3, Pykm Keywords Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL D38379; BAA07457.1; -; mRNA EMBL X97047; CAA65761.1; -; mRNA EMBL AK002341; BAB22025.1; -; mRNA EMBL AK135397; BAE22519.1; -; mRNA EMBL AK151724; BAE30642.1; -; mRNA EMBL AK153483; BAE32031.1; -; mRNA EMBL AK155110; BAE33055.1; -; mRNA EMBL AK155655; BAE33370.1; -; mRNA EMBL AK170892; BAE42098.1; -; mRNA EMBL AK168943; BAE40751.1; -; mRNA EMBL AK171023; BAE42192.1; -; mRNA EMBL AK171033; BAE42199.1; -; mRNA EMBL AC160637; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL BC016619; AAH16619.1; -; mRNA EMBL BC094663; AAH94663.1; -; mRNA IPI IPI00407130; -; . IPI IPI00845840; -; . PIR S55921; S55921; . RefSeq NP_035229.2; NM_011099.2; . UniGene Mm.326167; -; . UniGene Mm.405069; -; . ProteinModelPortal P52480; -; . SMR P52480; 13-531; . IntAct P52480; 4; . MINT MINT-1850796; -; . STRING P52480; -; . PhosphoSite P52480; -; . SWISS-2DPAGE P52480; -; . PMMA-2DPAGE P52480; -; . REPRODUCTION-2DPAGE IPI00407130; -; . REPRODUCTION-2DPAGE P52480; -; . PRIDE P52480; -; . Ensembl ENSMUST00000034834; ENSMUSP00000034834; ENSMUSG00000032294; . Ensembl ENSMUST00000163694; ENSMUSP00000128770; ENSMUSG00000032294; . GeneID 18746; -; . KEGG mmu:18746; -; . UCSC uc009pyf.1; mouse; . UCSC uc009pyi.1; mouse; . CTD 5315; -; . MGI MGI:97591; Pkm2; . eggNOG roNOG11660; -; . GeneTree ENSGT00390000008859; -; . HOVERGEN HBG000941; -; . InParanoid P52480; -; . OMA PANEGNT; -; . OrthoDB EOG40GCQJ; -; . PhylomeDB P52480; -; . NextBio 294905; -; . ArrayExpress P52480; -; . Bgee P52480; -; . CleanEx MM_PKM2; -; . Genevestigator P52480; -; . GermOnline ENSMUSG00000032294; Mus musculus; . GO GO:0019861; C:flagellum; IDA:MGI; . GO GO:0005739; C:mitochondrion; IDA:MGI; . GO GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0000287; F:magnesium ion binding; IEA:InterPro; . GO GO:0030955; F:potassium ion binding; IEA:InterPro; . GO GO:0005515; F:protein binding; IPI:IntAct; . GO GO:0004743; F:pyruvate kinase activity; IDA:MGI; . GO GO:0006096; P:glycolysis; IDA:MGI; . InterPro IPR001697; Pyr_Knase; . InterPro IPR015813; Pyrv/PenolPyrv_Kinase; . InterPro IPR011037; Pyrv_Knase-like_insert_dom; . InterPro IPR015794; Pyrv_Knase_a/b; . InterPro IPR018209; Pyrv_Knase_AS; . InterPro IPR015793; Pyrv_Knase_brl; . InterPro IPR015795; Pyrv_Knase_C; . InterPro IPR015806; Pyrv_Knase_insert_dom; . Gene3D G3DSA:2.40.33.10; PK_B_barrel_like; 1; . Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; . Gene3D G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; . PANTHER PTHR11817; Pyruvate_kinase; 1; . Pfam PF00224; PK; 1; . Pfam PF02887; PK_C; 1; . PRINTS PR01050; PYRUVTKNASE; . SUPFAM SSF50800; PK_B_barrel_like; 1; . SUPFAM SSF52935; Pyruvate_kinase; 1; . SUPFAM SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; . TIGRFAMs TIGR01064; Pyruv_kin; 1; . PROSITE PS00110; PYRUVATE_KINASE; 1; . SWISS-2DPAGE (search AC)