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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: LIVJ_ECOLI |
SWISS-2DPAGE: LIVJ_ECOLI
LIVJ_ECOLI:
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ID LIVJ_ECOLI; STANDARD; 2DG.
AC P0AD96; P02917;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 31-MAR-2004, 2D annotation version 6.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor;.
GN Name=livJ; OrderedLocusNames=b3460, JW3425;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI4.5-5.5, ECOLI5.5-6.7, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI4.5-5.5, ECOLI5.5-6.7, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
RA Bloch P.L., Phillips T.A., Neidhardt F.C.;
RT ''''''Protein identifications of O'Farrell two-dimensional gels:
RT locations of 81 Escherichia coli proteins'';'';'';
RL J. Bacteriol. 141(1):1409-1420(1980).
RN [3]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [4]
RP PROTEIN EXPRESSION.
RX MEDLINE=92138602; PubMed=1346534; [NCBI, Expasy, EBI, Israel, Japan]
RA Ernsting B.R., Atkinson M.R., Ninfa A.J., Matthews R.G.;
RT ''''''Characterization of the regulon controlled by the
RT leucine-responsive regulatory protein in Escherichia coli'';'';'';
RL J. Bacteriol. 174(1):1109-1118(1992).
RN [5]
RP PROTEIN EXPRESSION.
RX MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
RA Gage D.J., Neidhardt F.C.;
RT ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT phosphorylation 2,4-dinitrophenol'';'';'';
RL J. Bacteriol. 175(1):7105-7108(1993).
RN [6]
RP PROTEIN EXPRESSION.
RX MEDLINE=93352420; PubMed=8349554; [NCBI, Expasy, EBI, Israel, Japan]
RA Sankar P., Hutton M.E., Vanbogelen R.A., Clark R.L., Neidhardt F.C.;
RT ''''''Expression analysis of cloned chromosomal segments of Escherichia coli'';'';'';
RL J. Bacteriol. 175(1):5145-5152(1993).
RN [7]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [8]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KZA=5.28/42043;
2D -!- EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [5]; IS REDUCED IN
2D A LRP+ STRAIN WHEN LEUCINE IS ADDED TO THE MEDIUM [4]; WAS ENCODED AND
2D EXPRESSED FROM PLASMID PFNK613L [6].
2D -!- MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D IDENTIFIED ON 2-D GELS BY BLOCH [2] AND VANBOGELEN [3].
2D -!- MASTER: ECOLI4.5-5.5;
2D -!- PI/MW: SPOT 2D-00151X=5.31/40301;
2D -!- PI/MW: SPOT 2D-0015E7=5.21/16972;
2D -!- PEPTIDE MASSES: SPOT 2D-00151X: 1021.46; 1638.76; 1766.85; 1784.9;
2D 2528.24; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting and microsequencing (EDIKV) [7].
2D -!- MASTER: ECOLI5.5-6.7;
2D -!- PI/MW: SPOT 2D-001UF5=5.73/30106;
2D -!- MAPPING: GEL MATCHING WITH ECOLI5-6 [7].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L3W=5.09/50544;
2D -!- PI/MW: SPOT 2D-001L4G=5.23/50424;
2D -!- PI/MW: SPOT 2D-001LHX=5.67/30753;
2D -!- PI/MW: SPOT 2D-001LNA=5.33/23392;
2D -!- PEPTIDE MASSES: SPOT 2D-001L3W: 862.449; 908.41; 1021.427;
2D 1361.551; 1638.668; 1707.623; 1762.67; 1766.758; 1784.785; 2069.772;
2D 2400.94; 2416.912; 2482.919; 2528.083; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L4G: 862.52; 908.484; 1021.503;
2D 1361.814; 1638.857; 1784.885; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001LHX: 862.423; 908.378; 1021.391;
2D 1361.494; 1638.597; 1766.684; 2400.761; 2416.705; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001LNA: 908.628; 1639.008; 1767.13;
2D 1785.173; 2401.435; 2417.411; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [7].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WMS=5.18/39439;
2D -!- PI/MW: SPOT 2D-001WN2=5.30/38773;
2D -!- PEPTIDE MASSES: SPOT 2D-001WMS: 858.48; 862.5; 891.45; 908.42;
2D 983.36; 1021.49; 1361.65; 1784.92; 1797.96; 2528.3; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WN2: 856.46; 862.47; 908.39; 983.25;
2D 1021.37; 1361.44; 1525.61; 1784.67; 1797.7; 2069.68; 2400.74; 2483.56;
2D 2527.84; 2756.02; 3144.1; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WMS: (R)TTGLDSDQGPTAAK(Y),140-153;
2D (K)GNANVVFFDGITAGEKDFSTLVAR(L),188-211; (K)NYDQVPANKPIVDAIK(A),274-289.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WN2: (K)QAVAVANKVVNDGIK(Y),80-94;
2D (R)TTGLDSDQGPTAAK(Y),140-153; (K)GNANVVFFDGITAGEKDFSTLVAR(L),188-211.
2D -!- EXPRESSION: decrease after benzoic acid treatment [8].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [8].
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DR 2DBase-Ecoli; P0AD96; LIVJ_ECOLI.
DR ECO2DBASE; D040.7; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P0AD96; LIVJ_ECOLI.
//
The following 2-D maps are available for this protein:
|
Escherichia coli
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Escherichia coli(4.5-5.5)
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Escherichia coli(5.5-6.7)
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|---|
Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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