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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: LMNA_HUMAN SWISS-2DPAGE:  LMNA_HUMAN LMNA_HUMAN General information about the entry View entry in simple text format Entry name LMNA_HUMAN Primary accession number P02545 Secondary accession number(s) P02546 integrated into SWISS-2DPAGE on  April 1, 2000 (release 12) 2D Annotations were last modified on May 15, 2003 (version 3) General Annotations were last modified on  May 19, 2011 (version 9) Name and origin of the protein Description RecName: Full=Prelamin-A/C; Contains: RecName: Full=Lamin-A/C; AltName: Full=70 kDa lamin; AltName: Full=Renal carcinoma antigen NY-REN-32; Flags: Precursor;. Gene name Name=LMNA Synonyms=LMN1 Annotated species Homo sapiens (Human) [TaxID: 9606] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. References [1]   MAPPING ON GEL MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan] Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F. ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'' Electrophoresis 21(1):3483-3487(2000) [2]   MAPPING ON GEL PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan] Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. ''''''Functional proteomic analysis of human nucleolus'';'';'' Mol. Biol. Cell. 13(1):4100-4109(2002) 2D PAGE maps for identified proteins How to interpret a protein NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells} Homo sapiens (Human) Tissue: Cervix carcinoma   map experimental info   protein estimated location   NUCLEOLI_HELA_1D_HUMAN MAP LOCATIONS: BAND 1D-001V8C: Mw=63488 MAPPING (identification): Peptide mass fingerprinting [2]. NUCLEI_LIVER_HUMAN {Soluble nuclear proteins and matrix from liver tissue} Homo sapiens (Human) Tissue: Liver   map experimental info   protein estimated location   NUCLEI_LIVER_HUMAN MAP LOCATIONS: SPOT 2D-001IZK: pI=7.15; Mw=68933   [identification data] SPOT 2D-001IZM: pI=7.00; Mw=68933   [identification data] SPOT 2D-001IZR: pI=7.11; Mw=68566   [identification data] SPOT 2D-001J2X: pI=7.11; Mw=59687   [identification data] SPOT 2D-001J35: pI=6.91; Mw=59370   [identification data] SPOT 2D-001J37: pI=7.04; Mw=59370   [identification data] MAPPING (identification): Peptide mass fingerprinting [1]. NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells} Homo sapiens (Human) Tissue: Cervix carcinoma   map experimental info   protein estimated location   NUCLEOLI_HELA_2D_HUMAN MAP LOCATIONS: SPOT 2D-001VG3: pI=6.76; Mw=75283   [identification data] SPOT 2D-001VI5: pI=6.68; Mw=64287   [identification data] MAPPING (identification): Peptide mass fingerprinting [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references REPRODUCTION-2DPAGE P02545; P02545. REPRODUCTION-2DPAGE IPI00216952; IPI00216952. REPRODUCTION-2DPAGE IPI00021405; IPI00021405. Siena-2DPAGE P02545; LMNA_HUMAN. UniProtKB/Swiss-Prot P02545; LMNA_HUMAN. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name LMNA_HUMAN Primary accession number P02545 Secondary accession number(s) D3DVB0 P02546 Q5TCJ2 Q5TCJ3 Q969I8 Q96JA2 Sequence was last modified on  March 20, 1987 (version 1) Annotations were last modified on  October 19, 2011 (version 165) Name and origin of the protein Description RecName: Full=Prelamin-A/C; Contains: RecName: Full=Lamin-A/C; AltName: Full=70 kDa lamin; AltName: Full=Renal carcinoma antigen NY-REN-32; Flags: Precursor; Gene name Name=LMNA Synonyms=LMN1 Encoded on Name=LMNA; Synonyms=LMN1 Keywords 3D-structure; Acetylation; Alternative splicing; Cardiomyopathy; Charcot-Marie-Tooth disease; Coiled coil; Complete proteome; Congenital muscular dystrophy; Direct protein sequencing; Disease mutation; Emery-Dreifuss muscular dystrophy; Intermediate filament; Isopeptide bond; Limb-girdle muscular dystrophy; Lipoprotein; Methylation; Neuropathy; Nucleus; Phosphoprotein; Prenylation; Reference proteome; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X03444; CAA27173.1; ALT_FRAME; mRNA EMBL X03445; CAA27174.1; -; mRNA EMBL M13451; AAA36164.1; -; mRNA EMBL M13452; AAA36160.1; -; mRNA EMBL AL135927; CAI15521.1; -; Genomic_DNA EMBL AL135927; CAI15522.1; -; Genomic_DNA EMBL CH471121; EAW52997.1; -; Genomic_DNA EMBL CH471121; EAW52999.1; -; Genomic_DNA EMBL BC000511; AAH00511.1; -; mRNA EMBL BC003162; AAH03162.1; -; mRNA EMBL BC014507; AAH14507.1; -; mRNA EMBL AF381029; AAK59326.1; -; mRNA IPI IPI00021405; -; . IPI IPI00216952; -; . IPI IPI00216953; -; . PIR A02961; VEHULA; . PIR A02962; VEHULC; . RefSeq NP_005563.1; NM_005572.3; . RefSeq NP_733821.1; NM_170707.2; . RefSeq NP_733822.1; NM_170708.2; . UniGene Hs.594444; -; . PDB 1IFR; X-ray; 1.40 A; A=435-552 PDB 1IVT; NMR; -; A=428-549 PDB 1X8Y; X-ray; 2.20 A; A=305-387 PDB 2XV5; X-ray; 2.40 A; A/B=328-398 PDB 3GEF; X-ray; 1.50 A; A/B/C/D=436-552 PDBsum 1IFR; -; . PDBsum 1IVT; -; . PDBsum 1X8Y; -; . PDBsum 2XV5; -; . PDBsum 3GEF; -; . ProteinModelPortal P02545; -; . SMR P02545; 313-386; 428-549; . IntAct P02545; 23; . MINT MINT-5003995; -; . STRING P02545; -; . PhosphoSite P02545; -; . SWISS-2DPAGE P02545; -; . REPRODUCTION-2DPAGE IPI00021405; -; . REPRODUCTION-2DPAGE IPI00216952; -; . REPRODUCTION-2DPAGE P02545; -; . PeptideAtlas P02545; -; . PRIDE P02545; -; . Ensembl ENST00000368300; ENSP00000357283; ENSG00000160789; . GeneID 4000; -; . KEGG hsa:4000; -; . UCSC uc001fnh.2; human; . UCSC uc001fni.2; human; . CTD 4000; -; . GeneCards GC01P127446; -; . HGNC HGNC:6636; LMNA; . HPA CAB004022; -; . HPA HPA006660; -; . MIM 115200; phenotype; . MIM 150330; gene; . MIM 151660; phenotype; . MIM 159001; phenotype; . MIM 176670; phenotype; . MIM 181350; phenotype; . MIM 212112; phenotype; . MIM 248370; phenotype; . MIM 275210; phenotype; . MIM 605588; phenotype; . MIM 610140; phenotype; . MIM 613205; phenotype; . neXtProt NX_P02545; -; . Orphanet 98853; Autosomal dominant Emery-Dreifuss muscular dystrophy; . Orphanet 264; Autosomal dominant limb-girdle muscular dystrophy type 1B; . Orphanet 98855; Autosomal recessive Emery-dreifuss muscular dystrophy; . Orphanet 98856; Charcot-Marie-Tooth disease type 2B1; . Orphanet 157973; Congenital muscular dystrophy due to lamine A/C deficiency; . Orphanet 154; Familial isolated dilated cardiomyopathy; . Orphanet 2348; Familial partial lipodystrophy; Dunnigan type; . Orphanet 168796; Heart-hand syndrome; Slovenian type; . Orphanet 137871; Laminopathy type Decaudain-Vigouroux; . Orphanet 54260; Left ventricular noncompaction; . Orphanet 1662; Lethal restrictive dermopathy; . Orphanet 90153; Mandibuloacral dysplasia with type A lipodystrophy; . Orphanet 740; Progeria; . Orphanet 83618; Severe dilated cardiomyopathy due to lamin A/C mutation; . Orphanet 902; Werner syndrome; . PharmGKB PA231; -; . eggNOG prNOG13164; -; . HOGENOM HBG716303; -; . HOVERGEN HBG013015; -; . InParanoid P02545; -; . OMA HCSGSGD; -; . OrthoDB EOG4S4PG2; -; . PhylomeDB P02545; -; . Pathway_Interaction_DB caspase_pathway; Caspase cascade in apoptosis; . Pathway_Interaction_DB faspathway; FAS signaling pathway (CD95); . Reactome REACT_15380; Diabetes pathways; . Reactome REACT_578; Apoptosis; . Reactome REACT_75792; Meiotic Synapsis; . NextBio 15692; -; . PMAP-CutDB P02545; -; . ArrayExpress P02545; -; . Bgee P02545; -; . CleanEx HS_LMNA; -; . Genevestigator P02545; -; . GermOnline ENSG00000160789; Homo sapiens; . GO GO:0005737; C:cytoplasm; IDA:HPA; . GO GO:0005638; C:lamin filament; TAS:UniProtKB; . GO GO:0005635; C:nuclear envelope; IDA:UniProtKB; . GO GO:0005654; C:nucleoplasm; TAS:Reactome; . GO GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB; . GO GO:0005515; F:protein binding; IPI:UniProtKB; . GO GO:0005198; F:structural molecule activity; IEA:InterPro; . GO GO:0006921; P:cellular component disassembly involved in apoptosis; TAS:Reactome; . GO GO:0071456; P:cellular response to hypoxia; IEP:UniProtKB; . GO GO:0030951; P:establishment or maintenance of microtubule cytoskeleton polarity; ISS:BHF-UCL; . GO GO:0007517; P:muscle organ development; IMP:UniProtKB; . GO GO:0090343; P:positive regulation of cell aging; IDA:UniProtKB; . GO GO:0042981; P:regulation of apoptosis; ISS:BHF-UCL; . GO GO:0030334; P:regulation of cell migration; ISS:BHF-UCL; . InterPro IPR016044; F; . InterPro IPR001664; IF; . InterPro IPR001322; IF_tail_C; . InterPro IPR018039; Intermediate_filament_CS; . PANTHER PTHR23239; IF; 1; . Pfam PF00038; Filament; 1; . Pfam PF00932; IF_tail; 1; . PROSITE PS00226; IF; 1; . SWISS-2DPAGE (search AC)