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Sample Preparation and Post-separation Analysis

M3K1_HUMAN:

ID   M3K1_HUMAN; STANDARD; 2DG.
AC   Q13233;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   01-DEC-2000, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Mitogen-activated protein kinase kinase kinase 1;
DE   EC=2.7.11.25; AltName: Full=MAPK/ERK kinase kinase 1; Short=MEK kinase 1;
DE   Short=MEKK 1;.
GN   Name=MAP3K1; Synonyms=MAPKKK1, MEKK, MEKK1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   DLD1_HUMAN, HEPG2_HUMAN, LIVER_HUMAN.
IM   DLD1_HUMAN, HEPG2_HUMAN, LIVER_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=97295311; PubMed=9150953; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R.,
RA   Hochstrasser D.F.;
RT   ''''''Simultaneous analysis of cyclin and oncogene expression using
RT   multiple monoclonal antibody immunoblots'';'';'';
RL   Electrophoresis 18(1):638-641(1997).
RN   [2]
RP   MAPPING ON GEL.
RA   Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA   Appel R.D., Hochstrasser D.F.;
RL   Submitted (JAN-1999) to SWISS-2DPAGE.
2D   -!- MASTER: DLD1_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001E13=6.81/46815;
2D   -!-   PI/MW: SPOT 2D-001E15=6.90/46815;
2D   -!-   PI/MW: SPOT 2D-001E1B=6.60/46694;
2D   -!-   PI/MW: SPOT 2D-001E1F=6.35/46694;
2D   -!-   MAPPING: IMMUNOBLOTTING [2].
2D   -!- MASTER: HEPG2_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0012IN=6.50/45349;
2D   -!-   PI/MW: SPOT 2D-0012IO=6.79/45349;
2D   -!-   PI/MW: SPOT 2D-0012IP=6.90/45096;
2D   -!-   PI/MW: SPOT 2D-0012IQ=6.93/44844;
2D   -!-   MAPPING: IMMUNOBLOTTING [1].
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-0010AE=6.77/43848;
2D   -!-   PI/MW: SPOT 2D-0010AF=6.60/43848;
2D   -!-   PI/MW: SPOT 2D-0010AG=7.07/43727;
2D   -!-   PI/MW: SPOT 2D-0010AH=6.41/43486;
2D   -!-   MAPPING: IMMUNOBLOTTING [1].
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DR   UniProtKB/Swiss-Prot; Q13233; M3K1_HUMAN.
//

The following 2-D maps are available for this protein:

	Colorectal adenocarcinoma cell line (DL-1)	HepG2	Liver

SWISS-2DPAGE (search AC)