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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: MALE_ECOLI




SWISS-2DPAGE:  MALE_ECOLI


MALE_ECOLI


General information about the entry
View entry in simple text format
Entry nameMALE_ECOLI
Primary accession numberP0AEX9
Secondary accession number(s) P02928
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onMay 15, 2003 (version 6)
General Annotations were last modified on November 9, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP; AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor;.
Gene nameName=malE
OrderedLocusNames=b4034, JW3994
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.08; Mw=41219  [identification data]

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (EE) [1].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.22; Mw=39098  [identification data]
pI=5.24; Mw=38549

MAPPING (identification):
MICROSEQUENCING (XVAA AND KIEE) [2];
SPOT 2D-00152X: MASS FINGERPRINTING [2].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.09; Mw=50544  [identification data]
pI=5.00; Mw=50085  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.20; Mw=38691  [identification data]

MAPPING (identification):
Tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASED036.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0AEX9; MALE_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMALE_ECOLI
Primary accession numberP0AEX9
Secondary accession number(s) P02928 Q2M6S0
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 70)
Name and origin of the protein
DescriptionRecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP; AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor;
Gene nameName=malE
OrderedLocusNames=b4034, JW3994
Encoded onName=malE; OrderedLocusNames=b4034, JW3994
Keywords3D-structure; Complete proteome; Direct protein sequencing; Periplasm; Reference proteome; Signal; Sugar transport; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00303; CAA23581.1; -; Genomic_DNA
EMBLU00006; AAC43128.1; -; Genomic_DNA
EMBLU00096; AAC77004.1; -; Genomic_DNA
EMBLAP009048; BAE78036.1; -; Genomic_DNA
EMBLJ01648; AAB59056.1; -; Genomic_DNA
EMBLM16181; AAA24102.1; -; Genomic_DNA
EMBLM12635; AAA24123.1; -; Genomic_DNA
EMBLM12647; AAA24135.1; -; Genomic_DNA
EMBLM12650; AAA24138.1; -; Genomic_DNA
EMBLM12653; AAA24115.1; -; Genomic_DNA
PIRA03428; JGECM; .
RefSeqNP_418458.1; NC_000913.2; .
PDB1A7L; X-ray; 2.90 A; A/B/C=27-389
PDB1ANF; X-ray; 1.67 A; A=27-396
PDB1DMB; X-ray; 1.80 A; A=27-396
PDB1EZ9; X-ray; 1.90 A; A/B=27-396
PDB1EZO; NMR; -; A=27-396
PDB1EZP; NMR; -; A=27-396
PDB1FQA; X-ray; 1.90 A; A=28-396
PDB1FQB; X-ray; 1.90 A; A=28-396
PDB1FQC; X-ray; 2.30 A; A=28-396
PDB1FQD; X-ray; 2.30 A; A=28-396
PDB1HSJ; X-ray; 2.30 A; A/B=27-396
PDB1IUD; X-ray; 2.70 A; A=27-396
PDB1JVX; X-ray; 2.50 A; A=27-396
PDB1JVY; X-ray; 1.90 A; A=27-396
PDB1JW4; X-ray; 2.00 A; A=27-396
PDB1JW5; X-ray; 2.00 A; A=27-396
PDB1LAX; X-ray; 1.85 A; A/C=27-396
PDB1LLS; X-ray; 1.80 A; A=27-396
PDB1MDP; X-ray; 2.30 A; 1/2=27-396
PDB1MDQ; X-ray; 1.90 A; A=27-396
PDB1MG1; X-ray; 2.50 A; A=31-396
PDB1MH3; X-ray; 2.10 A; A=27-392
PDB1MH4; X-ray; 2.30 A; A=27-392
PDB1MPB; X-ray; 2.00 A; A=27-396
PDB1MPC; X-ray; 2.10 A; A=27-396
PDB1MPD; X-ray; 2.30 A; A=27-396
PDB1N3W; X-ray; 2.60 A; A=27-396
PDB1N3X; X-ray; 2.50 A; A=27-396
PDB1NL5; X-ray; 2.10 A; A=27-396
PDB1NMU; X-ray; 2.31 A; A/C=27-392
PDB1OMP; X-ray; 1.80 A; A=27-396
PDB1PEB; X-ray; 2.60 A; A=27-396
PDB1R6Z; X-ray; 2.80 A; A/P/Z=27-392
PDB1SVX; X-ray; 2.24 A; B=27-392
PDB1T0K; X-ray; 3.24 A; A=27-392
PDB1Y4C; X-ray; 1.90 A; A=27-396
PDB1YTV; X-ray; 1.80 A; A/B=27-392
PDB1ZIU; X-ray; 2.00 A; A=27-396
PDB1ZJL; X-ray; 2.00 A; A=27-396
PDB1ZKB; X-ray; 2.20 A; A=27-396
PDB1ZMG; X-ray; 2.50 A; A=27-396
PDB2D21; NMR; -; A=27-396
PDB2H25; NMR; -; A=29-396
PDB2KLF; NMR; -; A=29-396
PDB2NVU; X-ray; 2.80 A; B=25-392
PDB2OBG; X-ray; 2.35 A; A=31-396
PDB2OK2; X-ray; 2.00 A; A/B=28-392
PDB2R6G; X-ray; 2.80 A; E=27-396
PDB2V93; NMR; -; A=27-396
PDB2VGQ; X-ray; 2.10 A; A=27-392
PDB2ZXT; X-ray; 3.00 A; A=27-392
PDB3A3C; X-ray; 2.50 A; A=29-392
PDB3C4M; X-ray; 1.95 A; A/B=26-392
PDB3CSB; X-ray; 2.00 A; A=31-396
PDB3CSG; X-ray; 1.80 A; A=31-396
PDB3DM0; X-ray; 2.40 A; A=27-392
PDB3EHS; X-ray; 2.76 A; A=26-392
PDB3EHT; X-ray; 3.40 A; A=26-392
PDB3EHU; X-ray; 1.96 A; A/B=26-392
PDB3F5F; X-ray; 2.65 A; A=27-392
PDB3G7V; X-ray; 1.86 A; A/B/C/D=27-392
PDB3G7W; X-ray; 1.75 A; A=27-392
PDB3H3G; X-ray; 1.94 A; A=26-392
PDB3H4Z; X-ray; 2.35 A; A/B/C=27-392
PDB3HPI; X-ray; 2.00 A; A/B=26-396
PDB3HST; X-ray; 2.25 A; A/C=27-392
PDB3IO4; X-ray; 3.63 A; A/B/C=27-392
PDB3IO6; X-ray; 3.70 A; A/B/C=27-384
PDB3IOR; X-ray; 3.60 A; A/B/C=27-392
PDB3IOT; X-ray; 3.50 A; A/B/C=27-392
PDB3IOU; X-ray; 3.70 A; A/B/C=27-392
PDB3IOV; X-ray; 3.70 A; A/B/C=27-392
PDB3IOW; X-ray; 3.50 A; A/B/C=27-392
PDB3KJT; X-ray; 1.50 A; A=26-396
PDB3L2J; X-ray; 3.24 A; A/B=29-392
PDB3LBS; X-ray; 2.15 A; A/B=29-390
PDB3LC8; X-ray; 2.00 A; A/B=29-390
PDB3MBP; X-ray; 1.70 A; A=27-396
PDB3MP1; X-ray; 2.60 A; A=27-392
PDB3MP6; X-ray; 1.48 A; A=27-392
PDB3MP8; X-ray; 1.92 A; A=27-392
PDB3MQ9; X-ray; 2.80 A; A/B/C/D/E/F/G/H=27-395
PDB3N94; X-ray; 1.80 A; A=26-392
PDB3O3U; X-ray; 1.50 A; N=28-384
PDB3PGF; X-ray; 2.10 A; A=27-392
PDB3PUV; X-ray; 2.40 A; E=27-396
PDB3PUW; X-ray; 2.30 A; E=27-396
PDB3PUX; X-ray; 2.30 A; E=27-396
PDB3PUY; X-ray; 3.10 A; E=27-396
PDB3PUZ; X-ray; 2.90 A; E=27-396
PDB3PV0; X-ray; 3.10 A; E=27-396
PDB3Q25; X-ray; 1.90 A; A=27-392
PDB3Q26; X-ray; 1.54 A; A=27-392
PDB3Q27; X-ray; 1.30 A; A=27-392
PDB3Q28; X-ray; 1.60 A; A=27-392
PDB3Q29; X-ray; 2.30 A; A/C=27-392
PDB3RLF; X-ray; 2.20 A; E=27-396
PDB4MBP; X-ray; 1.70 A; A=27-396
PDBsum1A7L; -; .
PDBsum1ANF; -; .
PDBsum1DMB; -; .
PDBsum1EZ9; -; .
PDBsum1EZO; -; .
PDBsum1EZP; -; .
PDBsum1FQA; -; .
PDBsum1FQB; -; .
PDBsum1FQC; -; .
PDBsum1FQD; -; .
PDBsum1HSJ; -; .
PDBsum1IUD; -; .
PDBsum1JVX; -; .
PDBsum1JVY; -; .
PDBsum1JW4; -; .
PDBsum1JW5; -; .
PDBsum1LAX; -; .
PDBsum1LLS; -; .
PDBsum1MDP; -; .
PDBsum1MDQ; -; .
PDBsum1MG1; -; .
PDBsum1MH3; -; .
PDBsum1MH4; -; .
PDBsum1MPB; -; .
PDBsum1MPC; -; .
PDBsum1MPD; -; .
PDBsum1N3W; -; .
PDBsum1N3X; -; .
PDBsum1NL5; -; .
PDBsum1NMU; -; .
PDBsum1OMP; -; .
PDBsum1PEB; -; .
PDBsum1R6Z; -; .
PDBsum1SVX; -; .
PDBsum1T0K; -; .
PDBsum1Y4C; -; .
PDBsum1YTV; -; .
PDBsum1ZIU; -; .
PDBsum1ZJL; -; .
PDBsum1ZKB; -; .
PDBsum1ZMG; -; .
PDBsum2D21; -; .
PDBsum2H25; -; .
PDBsum2KLF; -; .
PDBsum2NVU; -; .
PDBsum2OBG; -; .
PDBsum2OK2; -; .
PDBsum2R6G; -; .
PDBsum2V93; -; .
PDBsum2VGQ; -; .
PDBsum2ZXT; -; .
PDBsum3A3C; -; .
PDBsum3C4M; -; .
PDBsum3CSB; -; .
PDBsum3CSG; -; .
PDBsum3DM0; -; .
PDBsum3EHS; -; .
PDBsum3EHT; -; .
PDBsum3EHU; -; .
PDBsum3F5F; -; .
PDBsum3G7V; -; .
PDBsum3G7W; -; .
PDBsum3H3G; -; .
PDBsum3H4Z; -; .
PDBsum3HPI; -; .
PDBsum3HST; -; .
PDBsum3IO4; -; .
PDBsum3IO6; -; .
PDBsum3IOR; -; .
PDBsum3IOT; -; .
PDBsum3IOU; -; .
PDBsum3IOV; -; .
PDBsum3IOW; -; .
PDBsum3KJT; -; .
PDBsum3L2J; -; .
PDBsum3LBS; -; .
PDBsum3LC8; -; .
PDBsum3MBP; -; .
PDBsum3MP1; -; .
PDBsum3MP6; -; .
PDBsum3MP8; -; .
PDBsum3MQ9; -; .
PDBsum3N94; -; .
PDBsum3O3U; -; .
PDBsum3PGF; -; .
PDBsum3PUV; -; .
PDBsum3PUW; -; .
PDBsum3PUX; -; .
PDBsum3PUY; -; .
PDBsum3PUZ; -; .
PDBsum3PV0; -; .
PDBsum3Q25; -; .
PDBsum3Q26; -; .
PDBsum3Q27; -; .
PDBsum3Q28; -; .
PDBsum3Q29; -; .
PDBsum3RLF; -; .
PDBsum4MBP; -; .
ProteinModelPortalP0AEX9; -; .
SMRP0AEX9; 27-385; .
DIPDIP-31871N; -; .
IntActP0AEX9; 6; .
TCDB3.A.1.1.1; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP0AEX9; -; .
ECO2DBASED036.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002502; EBESCP00000002502; EBESCG00000002041; .
EnsemblBacteriaEBESCT00000014255; EBESCP00000013546; EBESCG00000013317; .
GeneID948538; -; .
GenomeReviewsAP009048_GR; JW3994; .
GenomeReviewsU00096_GR; b4034; .
KEGGecj:JW3994; -; .
KEGGeco:b4034; -; .
EchoBASEEB0549; -; .
EcoGeneEG10554; malE; .
eggNOGCOG2182; -; .
GeneTreeEBGT00050000010961; -; .
HOGENOMHBG727666; -; .
OMAPKSWEEI; -; .
ProtClustDBPRK09474; -; .
BioCycEcoCyc:MALE-MONOMER; -; .
GenevestigatorP0AEX9; -; .
GOGO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:EcoCyc; .
GOGO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell; .
GOGO:0015609; F:maltooligosaccharide-importing ATPase activity; IGI:EcoCyc; .
GOGO:0015423; F:maltose-transporting ATPase activity; IDA:EcoCyc; .
GOGO:0042956; P:maltodextrin transport; IGI:EcoCyc; .
InterProIPR006060; Maltose-bd; .
InterProIPR006059; Solute-bd_1_bac; .
InterProIPR006061; Solute-bd_1_bac_CS; .
PfamPF01547; SBP_bac_1; 1; .
PRINTSPR00181; MALTOSEBP; .
PROSITEPS01037; SBP_BACTERIAL_1; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server