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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: MALE_ECOLI




SWISS-2DPAGE: MALE_ECOLI



MALE_ECOLI:

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ID   MALE_ECOLI; STANDARD; 2DG.
AC   P0AEX9; P02928;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   15-MAY-2003, 2D annotation version 6.
DT   09-NOV-2011, general annotation version 12.
DE   RecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP;
DE   AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor;.
GN   Name=malE; OrderedLocusNames=b4034, JW3994;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI4.5-5.5, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [3]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000L0A=5.08/41219;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000L0A: B=11.4, Z=10.5, S=4.7,
2D         H=0.8, G=10, T=5.4, A=12.2, P=5.5, Y=2.7, R=3.1, V=6.5, M=1.3, I=5.8,
2D         L=9, F=4.8, K=6.4;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (EE) [1].
2D   -!- MASTER: ECOLI4.5-5.5;
2D   -!-   PI/MW: SPOT 2D-00152X=5.22/39098;
2D   -!-   PI/MW: SPOT 2D-00153H=5.24/38549;
2D   -!-   PEPTIDE MASSES: SPOT 2D-00152X: 959.74; 1057.85; 1189.96; 1267.9;
2D         1336.9; 1767.19; 1914.17; 2097.37; 2139.51; TRYPSIN.
2D   -!-   MAPPING: MICROSEQUENCING (XVAA AND KIEE) [2]; SPOT 2D-00152X: MASS
2D         FINGERPRINTING [2].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001L3W=5.09/50544;
2D   -!-   PI/MW: SPOT 2D-001L61=5.00/50085;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L3W: 959.53; 1267.698; 1766.898;
2D         1891.007; 2139.147; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001L61: 959.495; 1057.542; 1189.69;
2D         1267.635; 1336.618; 1337.676; 1423.719; 1766.866; 1891.056; 1897.809;
2D         2097.038; 2110.063; 2213.082; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WN8=5.20/38691;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WN8: (K)AGLTFLVDLIK(N),216-226;
2D         (K)VNYGVTVLPTFK(G),266-277; (R)TAVINAASGR(Q),371-380.
2D   -!-   MAPPING: Tandem mass spectrometry [3].
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CC   This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
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CC   its content is in no way modified and this statement is not removed.
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CC   ---------------------------------------------------------------------------
DR   ECO2DBASE; D036.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0AEX9; MALE_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(4.5-5.5)
ECOLI4.5-5.5
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server