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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: METK_ECOLI




SWISS-2DPAGE:  METK_ECOLI


METK_ECOLI


General information about the entry
View entry in simple text format
Entry nameMETK_ECOLI
Primary accession numberP0A817
Secondary accession number(s) P04384
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 19)
Name and origin of the protein
DescriptionRecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase; EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase;.
Gene nameName=metK
Synonyms=metX
OrderedLocusNames=b2942, JW2909
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.03; Mw=45059

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.13; Mw=43079  [identification data]
pI=5.00; Mw=30312  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0A817; METK_ECOLI.
ECO2DBASEC044.6; 6TH EDITION.
UniProtKB/Swiss-ProtP0A817; METK_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMETK_ECOLI
Primary accession numberP0A817
Secondary accession number(s) P04384 P30869 Q2M9Q1
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 74)
Name and origin of the protein
DescriptionRecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase; EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase;
Gene nameName=metK
Synonyms=metX
OrderedLocusNames=b2942, JW2909
Encoded onName=metK; Synonyms=metX; OrderedLocusNames=b2942, JW2909
Keywords3D-structure; Acetylation; ATP-binding; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK02129; AAA24164.1; -; Genomic_DNA
EMBLU28377; AAA69109.1; -; Genomic_DNA
EMBLU00096; AAC75979.1; -; Genomic_DNA
EMBLAP009048; BAE77005.1; -; Genomic_DNA
EMBLM98266; AAB05197.1; -; Genomic_DNA
EMBLM31770; AAA24645.2; -; Genomic_DNA
PIRE65079; SYECSM; .
RefSeqNP_417417.1; NC_000913.2; .
PDB1FUG; X-ray; 3.20 A; A/B=2-384
PDB1MXA; X-ray; 2.80 A; A=2-384
PDB1MXB; X-ray; 2.80 A; A=2-384
PDB1MXC; X-ray; 3.00 A; A=2-384
PDB1P7L; X-ray; 2.50 A; A/B/C/D=2-383
PDB1RG9; X-ray; 2.50 A; A/B/C/D=2-383
PDB1XRA; X-ray; 3.00 A; A=2-384
PDB1XRB; X-ray; 3.00 A; A=2-384
PDB1XRC; X-ray; 3.00 A; A=2-384
PDBsum1FUG; -; .
PDBsum1MXA; -; .
PDBsum1MXB; -; .
PDBsum1MXC; -; .
PDBsum1P7L; -; .
PDBsum1RG9; -; .
PDBsum1XRA; -; .
PDBsum1XRB; -; .
PDBsum1XRC; -; .
ProteinModelPortalP0A817; -; .
SMRP0A817; 2-384; .
DIPDIP-35672N; -; .
IntActP0A817; 37; .
MINTMINT-1233798; -; .
PhosSiteP0A817; -; .
SWISS-2DPAGEP0A817; -; .
2DBase-EcoliP0A817; -; .
ECO2DBASEC044.6; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003134; EBESCP00000003134; EBESCG00000002570; .
EnsemblBacteriaEBESCT00000018096; EBESCP00000017387; EBESCG00000017152; .
GeneID945389; -; .
GenomeReviewsAP009048_GR; JW2909; .
GenomeReviewsU00096_GR; b2942; .
KEGGecj:JW2909; -; .
KEGGeco:b2942; -; .
EchoBASEEB0584; -; .
EcoGeneEG10589; metK; .
eggNOGCOG0192; -; .
GeneTreeEBGT00050000011099; -; .
HOGENOMHBG443662; -; .
OMAIYYAHRL; -; .
ProtClustDBPRK05250; -; .
BioCycEcoCyc:S-ADENMETSYN-MONOMER; -; .
BioCycMetaCyc:S-ADENMETSYN-MONOMER; -; .
GenevestigatorP0A817; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004478; F:methionine adenosyltransferase activity; IEA:EC; .
GOGO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW; .
GOGO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:InterPro; .
HAMAPMF_00086; S-AdoMet_synth1; 1; -
InterProIPR022631; ADOMET_SYNTHASE_CS; .
InterProIPR022630; S-AdoMet_synt_C; .
InterProIPR022629; S-AdoMet_synt_central; .
InterProIPR022628; S-AdoMet_synt_N; .
InterProIPR002133; S-AdoMet_synthetase; .
InterProIPR022636; S-AdoMet_synthetase_sfam; .
PANTHERPTHR11964; S-AdoMet_synt; 1; .
PfamPF02773; S-AdoMet_synt_C; 1; .
PfamPF02772; S-AdoMet_synt_M; 1; .
PfamPF00438; S-AdoMet_synt_N; 1; .
PIRSFPIRSF000497; MAT; 1; .
SUPFAMSSF55973; S-AdoMet_synt; 3; .
TIGRFAMsTIGR01034; MetK; 1; .
PROSITEPS00376; ADOMET_SYNTHASE_1; 1; .
PROSITEPS00377; ADOMET_SYNTHASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server