Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

O00567:

ID   NOP56_HUMAN; STANDARD; 2DG.
AC   O00567;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   15-MAY-2003, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 9.
DE   RecName: Full=Nucleolar protein 56; AltName: Full=Nucleolar protein 5A;.
GN   Name=NOP56; Synonyms=NOL5A;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V87=73806;
1D   -!-   MW: BAND 1D-001V88=70605;
1D   -!-   MW: BAND 1D-001V89=68364;
1D   -!-   PEPTIDE MASSES: BAND 1D-001V88: 918.5; 952.5; 1017.5; 1188.6;
1D         1239.6; 1311.7; 1439.8; 1857; 1872; 2575.4; TRYPSIN.
1D   -!-   PEPTIDE MASSES: BAND 1D-001V89: 918.5; 952.5; 1007.6; 1017.5;
1D         1188.6; 1239.6; 1311.7; 1376.7; 1822.9; 1857; 1873; 2353.3; 2575.4;
1D         3229.6; TRYPSIN.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V87:
1D         (K)EVEEISLLQPQVEESVLNLGK(F),21-41; (K)MoxSQVAPSLSALIGEAVGAR(L),289-307;
1D         (K)MSQVAPSLSALIGEAVGAR(L),289-307; (R)IDCFSEVPTSVFGEK(L),382-396.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V88: (R)LLLETHLPSK(K),78-87.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V89:
1D         (K)EVEEISLLQPQVEESVLNLGK(F),21-41;
1D         (R)LVAFCPFASSQVALENANAVSEGVVHEDLR(L),48-77; (R)LLLETHLPSK(K),78-87;
1D         (R)LHFHNLVK(G),127-134; (K)AQLGLGHSYSR(A),144-154;
1D         (R)EWYGYHFPELVK(I),191-202; (R)LAQFIGNR(R),213-220;
1D         (R)SSMoxGMoxDISAIDLINIESFSSR(V),250-270;
1D         (K)MoxSQVAPSLSALIGEAVGAR(L),289-307; (K)MSQVAPSLSALIGEAVGAR(L),289-307;
1D         (R)LIAHAGSLTNLAK(Y),308-320; (K)YPASTVQILGAEK(A),321-333; (R)IDCFSEVPTSVFGEK(L),382-396.
1D   -!-   MAPPING: Tandem mass spectrometry [1]; BANDS 1V88, 1V89: PEPTIDE
1D         MASS FINGERPRINTING [1].
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DR   UniProtKB/Swiss-Prot; O00567; NOP56_HUMAN.
//

The following 2-D map is available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)