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Sample Preparation and Post-separation Analysis

O15160:

ID   RPAC1_HUMAN; STANDARD; 2DG.
AC   O15160;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=DNA-directed RNA polymerases I and III subunit RPAC1;
DE   Short=DNA-directed RNA polymerase I subunit C; Short=RNA polymerases I
DE   and III subunit AC1; AltName: Full=AC40; AltName: Full=DNA-directed RNA
DE   polymerases I and III 40 kDa polypeptide; Short=RPA40; AltName:
DE   Full=RPA39; AltName: Full=RPC40;.
GN   Name=POLR1C; Synonyms=POLR1E;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8V=42495;
1D   -!-   MW: BAND 1D-001V8X=41811;
1D   -!-   PEPTIDE MASSES: BAND 1D-001V8V: 975.6; 1098.6; 1201.7; 1851.8;
1D         2084.1; 2294.2; 3166.5; TRYPSIN.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8V: (R)ILLAEVPTMAVEK(V),79-91; (R)LLPDITLLEPVEGEAAEELSR(C),235-255.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8X: (R)ILLAEVPTMoxAVEK(V),79-91;
1D         (R)NQGDEEGTEIDTLQFR(L),126-141; (R)LLPDITLLEPVEGEAAEELSR(C),235-255.
1D   -!-   MAPPING: BAND 1D-001V8V: PEPTIDE MASS FINGERPRINTING [1]; Tandem
1D         mass spectrometry [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VOK=5.50/42256;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VOK: 974.6; 1098.6; 1201.7; 1413.8;
2D         1429.7; 1467.7; 1851.8; 2084.1; 2294.2; 2870.6; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
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DR   REPRODUCTION-2DPAGE; IPI00217386; IPI00217386.
DR   UniProtKB/Swiss-Prot; O15160; RPAC1_HUMAN.
//

The following 2-D maps are available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells	2D-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)