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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: O95602




SWISS-2DPAGE:  O95602


O95602


General information about the entry
View entry in simple text format
Entry nameRPA1_HUMAN
Primary accession numberO95602
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 5)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase I subunit RPA1; Short=RNA polymerase I subunit A1; EC=2.7.7.6; AltName: Full=A190; AltName: Full=DNA-directed RNA polymerase I largest subunit; AltName: Full=DNA-directed RNA polymerase I subunit A; AltName: Full=RNA polymerase I 194 kDa subunit; Short=RPA194;.
Gene nameName=POLR1A
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
Comments
  • SUBUNIT: EACH CLASS OF RNA POLYMERASE IS ASSEMBLED FROM 9 TO 14 DIFFERENT POLYPEPTIDES. THIS SUBUNIT IS THE LARGEST COMPONENT OF RNA POLYMERASE I
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_1D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_1D_HUMAN

MAP LOCATIONS:
Mw=94000  [identification data]
Mw=94000  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtO95602; RPA1_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRPA1_HUMAN
Primary accession numberO95602
Secondary accession number(s) B7Z7T0 D6W5M0 Q0VG05 Q9UEH0 Q9UFT9
Sequence was last modified on November 25, 2008 (version 2)
Annotations were last modified on October 19, 2011 (version 106)
Name and origin of the protein
DescriptionRecName: Full=DNA-directed RNA polymerase I subunit RPA1; Short=RNA polymerase I subunit A1; EC=2.7.7.6; AltName: Full=A190; AltName: Full=DNA-directed RNA polymerase I largest subunit; AltName: Full=DNA-directed RNA polymerase I subunit A; AltName: Full=RNA polymerase I 194 kDa subunit; Short=RPA194;
Gene nameName=POLR1A
Encoded onName=POLR1A
KeywordsComplete proteome; DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transferase; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU33460; AAC99959.1; ALT_FRAME; mRNA
EMBLU33823; AAD09356.1; ALT_FRAME; Transcribed_RNA
EMBLU33824; AAD09356.1; JOINED; Transcribed_RNA
EMBLAK302458; BAH13716.1; -; mRNA
EMBLCH471053; EAW99467.1; -; Genomic_DNA
EMBLCH471053; EAW99469.1; -; Genomic_DNA
EMBLBC117173; AAI17174.1; -; mRNA
EMBLBC126303; AAI26304.1; -; mRNA
EMBLAL117467; CAB55942.1; -; mRNA
IPIIPI00031960; -; .
PIRT17252; T17252; .
RefSeqNP_056240.2; NM_015425.3; .
UniGeneHs.531818; -; .
ProteinModelPortalO95602; -; .
SMRO95602; 1574-1718; .
DIPDIP-27537N; -; .
IntActO95602; 8; .
MINTMINT-111492; -; .
STRINGO95602; -; .
PhosphoSiteO95602; -; .
SWISS-2DPAGEO95602; -; .
PRIDEO95602; -; .
EnsemblENST00000263857; ENSP00000263857; ENSG00000068654; .
GeneID25885; -; .
KEGGhsa:25885; -; .
CTD25885; -; .
GeneCardsGC02M086165; -; .
H-InvDBHIX0002233; -; .
HGNCHGNC:17264; POLR1A; .
HPACAB010145; -; .
neXtProtNX_O95602; -; .
PharmGKBPA134891380; -; .
eggNOGprNOG08294; -; .
HOGENOMHBG598940; -; .
HOVERGENHBG017741; -; .
InParanoidO95602; -; .
OMAVPPTRFR; -; .
OrthoDBEOG41VK23; -; .
PhylomeDBO95602; -; .
ReactomeREACT_1788; Transcription; .
PMAP-CutDBO95602; -; .
ArrayExpressO95602; -; .
BgeeO95602; -; .
CleanExHS_POLR1A; -; .
GenevestigatorO95602; -; .
GermOnlineENSG00000068654; Homo sapiens; .
GOGO:0005736; C:DNA-directed RNA polymerase I complex; NAS:UniProtKB; .
GOGO:0005654; C:nucleoplasm; TAS:Reactome; .
GOGO:0003677; F:DNA binding; IEA:InterPro; .
GOGO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
GOGO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome; .
GOGO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome; .
GOGO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome; .
InterProIPR015699; DNA-dir_RNA_pol1_lsu; .
InterProIPR000722; RNA_pol_asu; .
InterProIPR006592; RNA_pol_N; .
InterProIPR007080; RNA_pol_Rpb1_1; .
InterProIPR007066; RNA_pol_Rpb1_3; .
InterProIPR007083; RNA_pol_Rpb1_4; .
InterProIPR007081; RNA_pol_Rpb1_5; .
PANTHERPTHR19376:SF11; RNA_polyI; 1; .
PfamPF04997; RNA_pol_Rpb1_1; 1; .
PfamPF00623; RNA_pol_Rpb1_2; 1; .
PfamPF04983; RNA_pol_Rpb1_3; 1; .
PfamPF05000; RNA_pol_Rpb1_4; 1; .
PfamPF04998; RNA_pol_Rpb1_5; 1; .
SMARTSM00663; RPOLA_N; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server