Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

ODP2_ECOLI:

ID   ODP2_ECOLI; STANDARD; 2DG.
AC   P06959;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of
DE   pyruvate dehydrogenase complex; EC=2.3.1.12; AltName:
DE   Full=Dihydrolipoamide acetyltransferase component of pyruvate
DE   dehydrogenase complex; AltName: Full=E2;.
GN   Name=aceF; OrderedLocusNames=b0115, JW0111;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=87194592; PubMed=3553157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Jones P.G., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Induction of proteins in response to low temperature in Escherichia coli'';'';'';
RL   J. Bacteriol. 169(1):2092-2095(1987).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=83160794; PubMed=6339477; [NCBI, Expasy, EBI, Israel, Japan]
RA   Smith M.W., Neidhardt F.C.;
RT   ''''''Proteins induced by aerobiosis in Escherichia coli'';'';'';
RL   J. Bacteriol. 154(1):344-350(1983).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: FORMS A 24-POLYPEPTIDE STRUCTURAL CORE WITH OCTAHEDRAL
CC         SYMMETRY
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KHD=5.01/77450;
2D   -!-   EXPRESSION: INCREASED DURING AEROBIC GROWTH [4]; INCREASED DURING
2D         THE ADJUSTMENT OF E. COLI TO GROWTH AT 10°C [3].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WDC=5.09/78105;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WDC: 877.47; 951.48; 1172.6; 1239.57;
2D         1277.66; 1419.75; 1519.85; 1932.96; TRYPSIN.
2D   -!-   EXPRESSION: increase after benzoic acid treatment [5].
2D   -!-   MAPPING: Peptide mass fingerprinting [5].
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DR   ECO2DBASE; C062.7; 6TH EDITION.
DR   ECO2DBASE; C070.0; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P06959; ODP2_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)