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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: OPPA_ECOLI |
SWISS-2DPAGE: OPPA_ECOLI
OPPA_ECOLI:
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ID OPPA_ECOLI; STANDARD; 2DG.
AC P23843;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 11.
DE RecName: Full=Periplasmic oligopeptide-binding protein; Flags: Precursor;.
GN Name=oppA; OrderedLocusNames=b1243, JW1235;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5.5-6.7, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI5.5-6.7, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL Electrophoresis 19(1):1960-1971(1998).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KMQ=5.70/57529;
2D -!- PI/MW: SPOT 2D-000KN0=5.64/56854;
2D -!- PI/MW: SPOT 2D-000KNL=5.93/56188;
2D -!- PI/MW: SPOT 2D-000KOT=5.44/54555;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000KN0: B=13.1, Z=10.8, S=6.4,
2D H=0.9, G=8, T=7.7, A=8.4, P=6.4, Y=3.9, R=4.3, V=7.8, M=1.4, I=4.5,
2D L=8.5, F=3.3, K=4.7;
2D -!- MAPPING: SPOTS 2D-000KMQ, 2D-000KN0, 2D-000KOT: AMINO ACID
2D COMPOSITION AND SEQUENCE TAG (ADVP) [3]; SPOT 2D-000KNL: AMINO ACID
2D COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY
2D VANBOGELEN [2].
2D -!- MASTER: ECOLI5.5-6.7;
2D -!- PI/MW: SPOT 2D-001U91=5.65/50915;
2D -!- MAPPING: GEL MATCHING WITH ECOLI5-6 [4].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001L2C=5.13/50973;
2D -!- PI/MW: SPOT 2D-001L2S=5.47/50921; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001L2T=5.63/50921;
2D -!- PEPTIDE MASSES: SPOT 2D-001L2C: 917.63; 958.722; 1080.711;
2D 1170.823; 1200.841; 1274.979; 1425.929; 1636.052; 1764.174; 2143.352;
2D 2232.603; 2337.54; 2606.706; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L2S: 917.462; 958.549; 1059.619;
2D 1080.525; 1187.698; 1200.659; 1273.704; 1635.805; 1763.894; 1791.892;
2D 2001.916; 2053.939; 2143.011; 2232.213; 2337.177; 2606.315; 2781.321;
2D 2874.477; 3727.719; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001L2T: 917.559; 958.649; 1059.728;
2D 1080.647; 1170.77; 1200.774; 1274.905; 1635.959; 1764.101; 2143.244;
2D 2232.448; 2337.422; 2606.568; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WH6=5.93/55635;
2D -!- PI/MW: SPOT 2D-001WHC=5.82/55131;
2D -!- PI/MW: SPOT 2D-001WI9=5.71/52681;
2D -!- PEPTIDE MASSES: SPOT 2D-001WH6: 992.59; 1020.52; 1094.55; 1274.75;
2D 1629.9; 2001.9; 2017.91; 2134.95; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WHC: 1030.54; 1094.53; 1425.73; 1629.88;
2D 2017.91; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001WI9: 937.48; 949.41; 1007.52; 1050.52;
2D 1325.63; 1327.76; 1526.77; 1890.89; TRYPSIN.
2D -!- EXPRESSION: increase after benzoic acid treatment [5].
2D -!- MAPPING: Peptide mass fingerprinting [5].
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DR 2DBase-Ecoli; P23843; OPPA_ECOLI.
DR UniProtKB/Swiss-Prot; P23843; OPPA_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(5.5-6.7)
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Escherichia coli(5-6)
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|---|
Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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