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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P00358 SWISS-2DPAGE:  P00358 P00358 General information about the entry View entry in simple text format Entry name G3P2_YEAST Primary accession number P00358 integrated into SWISS-2DPAGE on  February 1, 1995 (release 1) 2D Annotations were last modified on October 1, 2001 (version 2) General Annotations were last modified on  May 19, 2011 (version 11) Name and origin of the protein Description RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2; Short=GAPDH 2; EC=1.2.1.12;. Gene name Name=TDH2 Synonyms=GPD2 OrderedLocusNames=YJR009C ORFNames=J1433 Annotated species Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. References [1]   MAPPING ON GEL MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan] Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F. ''''''The yeast SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):556-565(1996) [2]   MAPPING ON GEL MEDLINE=86059345; PubMed=2999100; [NCBI, Expasy, EBI, Israel, Japan] McAlister L., Holland M.J. ''''''Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes'';'';'' J. Biol. Chem. 260(1):15019-15027(1985) Comments SUBUNIT: HOMOTETRAMER 2D PAGE maps for identified proteins How to interpret a protein YEAST {Saccharomyces cerevisiae} Saccharomyces cerevisiae (Baker's yeast)   map experimental info   protein estimated location   YEAST MAP LOCATIONS: SPOT 2D-000JIE: pI=6.65; Mw=32035 SPOT 2D-000JIK: pI=6.72; Mw=31954 SPOT 2D-000JIL: pI=6.81; Mw=31954 SPOT 2D-000JIM: pI=6.77; Mw=31793 SPOT 2D-000JU5: pI=6.59; Mw=20618 SPOT 2D-000JYO: pI=6.73; Mw=12009 MAPPING (identification): AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY MCALLISTER AND HOLLAND [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references COMPLUYEAST-2DPAGE P00358. UniProtKB/Swiss-Prot P00358; G3P2_YEAST. YEPD 4270. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name G3P2_YEAST Primary accession number P00358 Secondary accession number(s) D6VWI4 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  October 19, 2011 (version 130) Name and origin of the protein Description RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2; Short=GAPDH 2; EC=1.2.1.12; Gene name Name=TDH2 Synonyms=GPD2 OrderedLocusNames=YJR009C ORFNames=J1433 Encoded on Name=TDH2; Synonyms=GPD2; OrderedLocusNames=YJR009C; ORFNames=J1433 Keywords Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X60157; CAA42725.1; -; Genomic_DNA EMBL V01301; CAA24608.1; ALT_SEQ; Genomic_DNA EMBL X87611; CAA60931.1; -; Genomic_DNA EMBL Z49509; CAA89531.1; -; Genomic_DNA EMBL BK006943; DAA08800.1; -; Genomic_DNA PIR S57024; DEBYG1; . RefSeq NP_012542.1; NM_001181666.1; . ProteinModelPortal P00358; -; . SMR P00358; 1-330; . DIP DIP-1951N; -; . IntAct P00358; 13; . MINT MINT-399020; -; . STRING P00358; -; . SWISS-2DPAGE P00358; -; . PeptideAtlas P00358; -; . EnsemblFungi YJR009C; YJR009C; YJR009C; . GeneID 853465; -; . KEGG sce:YJR009C; -; . NMPDR fig|4932.3.peg.3516; -; . SGD S000003769; TDH2; . eggNOG fuNOG04196; -; . GeneTree EFGT00050000004847; -; . HOGENOM HBG571736; -; . OMA CSINEIS; -; . OrthoDB EOG4578GC; -; . PhylomeDB P00358; -; . NextBio 974053; -; . Genevestigator P00358; -; . GermOnline YJR009C; Saccharomyces cerevisiae; . GO GO:0009277; C:fungal-type cell wall; IDA:SGD; . GO GO:0005811; C:lipid particle; IDA:SGD; . GO GO:0005739; C:mitochondrion; IDA:SGD; . GO GO:0001950; C:plasma membrane enriched fraction; IDA:SGD; . GO GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:SGD; . GO GO:0051287; F:NAD binding; IEA:InterPro; . GO GO:0006915; P:apoptosis; IMP:SGD; . GO GO:0006094; P:gluconeogenesis; IEP:SGD; . GO GO:0006096; P:glycolysis; IEP:SGD; . GO GO:0072593; P:reactive oxygen species metabolic process; IMP:SGD; . InterPro IPR020831; GlycerAld/Erythrose_P_DH; . InterPro IPR020830; GlycerAld_3-P_DH_AS; . InterPro IPR020829; GlycerAld_3-P_DH_cat; . InterPro IPR020828; GlycerAld_3-P_DH_NAD(P)-bd; . InterPro IPR006424; Glyceraldehyde-3-P_DH_1; . InterPro IPR016040; NAD(P)-bd_dom; . Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1; . PANTHER PTHR10836; GAP_DH; 1; . Pfam PF02800; Gp_dh_C; 1; . Pfam PF00044; Gp_dh_N; 1; . PIRSF PIRSF000149; GAP_DH; 1; . PRINTS PR00078; G3PDHDRGNASE; . SMART SM00846; Gp_dh_N; 1; . TIGRFAMs TIGR01534; GAPDH-I; 1; . PROSITE PS00071; GAPDH; 1; . SWISS-2DPAGE (search AC)