Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P00359:

ID   G3P3_YEAST; STANDARD; 2DG.
AC   P00359;
DT   01-FEB-1995, integrated into SWISS-2DPAGE (release 1).
DT   01-OCT-2001, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 3; Short=GAPDH 3; EC=1.2.1.12;.
GN   Name=TDH3; Synonyms=GPD3; OrderedLocusNames=YGR192C; ORFNames=G7576;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
MT   YEAST.
IM   YEAST.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch
RA   A., Hughes G.J., Hochstrasser D.F.;
RT   ''''''The yeast SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):556-565(1996).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=86059345; PubMed=2999100; [NCBI, Expasy, EBI, Israel, Japan]
RA   McAlister L., Holland M.J.;
RT   ''''''Isolation and characterization of yeast strains carrying mutations
RT   in the glyceraldehyde-3-phosphate dehydrogenase genes'';'';'';
RL   J. Biol. Chem. 260(1):15019-15027(1985).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: YEAST;
2D   -!-   PI/MW: SPOT 2D-000JH9=6.81/32772;
2D   -!-   PI/MW: SPOT 2D-000JHL=6.73/32772;
2D   -!-   PI/MW: SPOT 2D-000JHS=6.88/32607;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND
2D         IDENTIFIED ON 2-D GELS BY MCALLISTER AND HOLLAND [2].
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DR   COMPLUYEAST-2DPAGE; P00359; G3P3_YEAST.
DR   UniProtKB/Swiss-Prot; P00359; G3P3_YEAST.
DR   YEPD; 4280.
//

The following 2-D map is available for this protein:

	Saccharomyces cerevisiae

SWISS-2DPAGE (search AC)