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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00582




SWISS-2DPAGE:  P00582


P00582


General information about the entry
View entry in simple text format
Entry nameDPO1_ECOLI
Primary accession numberP00582
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=DNA polymerase I; Short=POL I; EC=2.7.7.7;.
Gene nameName=polA
Synonyms=resA
OrderedLocusNames=b3863, JW3835
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: MONOMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.37; Mw=108286

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
ECO2DBASEF113.0; 6TH EDITION.
UniProtKB/Swiss-ProtP00582; DPO1_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDPO1_ECOLI
Primary accession numberP00582
Secondary accession number(s) Q2M8G1
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 136)
Name and origin of the protein
DescriptionRecName: Full=DNA polymerase I; Short=POL I; EC=2.7.7.7;
Gene nameName=polA
Synonyms=resA
OrderedLocusNames=b3863, JW3835
Encoded onName=polA; Synonyms=resA; OrderedLocusNames=b3863, JW3835
Keywords3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00317; CAA23607.1; -; Genomic_DNA
EMBLL19201; AAB02998.1; -; Genomic_DNA
EMBLU00096; AAC76861.1; -; Genomic_DNA
EMBLAP009048; BAE77445.1; -; Genomic_DNA
EMBLJ01663; AAA24402.1; -; Genomic_DNA
EMBLJ01664; AAA24404.1; -; Genomic_DNA
PIRA92360; DJECI; .
RefSeqNP_418300.1; NC_000913.2; .
PDB1D8Y; X-ray; 2.08 A; A=325-928
PDB1D9D; X-ray; 2.18 A; A=325-928
PDB1D9F; X-ray; 3.00 A; A=325-928
PDB1DPI; X-ray; 2.80 A; A=324-928
PDB1KFD; X-ray; 3.90 A; A=324-928
PDB1KFS; X-ray; 2.10 A; A=325-928
PDB1KLN; X-ray; 3.20 A; A=324-928
PDB1KRP; X-ray; 2.20 A; A=325-928
PDB1KSP; X-ray; 2.30 A; A=325-928
PDB1QSL; X-ray; 2.20 A; A=325-928
PDB2KFN; X-ray; 2.03 A; A=325-928
PDB2KFZ; X-ray; 2.03 A; A=325-928
PDB2KZM; X-ray; 2.60 A; A=325-928
PDB2KZZ; X-ray; 2.25 A; A=325-928
PDBsum1D8Y; -; .
PDBsum1D9D; -; .
PDBsum1D9F; -; .
PDBsum1DPI; -; .
PDBsum1KFD; -; .
PDBsum1KFS; -; .
PDBsum1KLN; -; .
PDBsum1KRP; -; .
PDBsum1KSP; -; .
PDBsum1QSL; -; .
PDBsum2KFN; -; .
PDBsum2KFZ; -; .
PDBsum2KZM; -; .
PDBsum2KZZ; -; .
ProteinModelPortalP00582; -; .
SMRP00582; 5-928; .
DIPDIP-10524N; -; .
IntActP00582; 28; .
MINTMINT-1225247; -; .
SWISS-2DPAGEP00582; -; .
ECO2DBASEF113.0; 6TH EDITION; .
PRIDEP00582; -; .
EnsemblBacteriaEBESCT00000001399; EBESCP00000001399; EBESCG00000001165; .
EnsemblBacteriaEBESCT00000014390; EBESCP00000013681; EBESCG00000013451; .
GeneID948356; -; .
GenomeReviewsAP009048_GR; JW3835; .
GenomeReviewsU00096_GR; b3863; .
KEGGecj:JW3835; -; .
KEGGeco:b3863; -; .
EchoBASEEB0739; -; .
EcoGeneEG10746; polA; .
eggNOGCOG0749; -; .
GeneTreeEBGT00050000009637; -; .
HOGENOMHBG284758; -; .
OMAPKGAPST; -; .
ProtClustDBPRK05755; -; .
BioCycEcoCyc:EG10746-MONOMER; -; .
BioCycMetaCyc:EG10746-MONOMER; -; .
DrugBankDB00548; Azelaic Acid; .
GenevestigatorP00582; -; .
GOGO:0005622; C:intracellular; IEA:InterPro; .
GOGO:0008408; F:3'-5' exonuclease activity; IEA:InterPro; .
GOGO:0008409; F:5'-3' exonuclease activity; IEA:InterPro; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW; .
GOGO:0006281; P:DNA repair; IEA:UniProtKB-KW; .
GOGO:0006260; P:DNA replication; IEA:UniProtKB-KW; .
InterProIPR002562; 3'-5'_exonuclease; .
InterProIPR020046; 5-3_exonucl_a-hlix_arch_N; .
InterProIPR020045; 5-3_exonuclease_C; .
InterProIPR002421; 5-3_exonuclease_N; .
InterProIPR020047; 5-3_exonuclease_SAM-fold_dom; .
InterProIPR019760; DNA-dir_DNA_pol_A_CS; .
InterProIPR001098; DNA-dir_DNA_pol_A_palm_dom; .
InterProIPR018320; DNA_polymerase_1; .
InterProIPR002298; DNA_polymerase_A; .
InterProIPR008918; HhH2; .
InterProIPR003583; Hlx-hairpin-Hlx_DNA-bd_motif; .
InterProIPR012337; RNaseH-like; .
PfamPF01367; 5_3_exonuc; 1; .
PfamPF02739; 5_3_exonuc_N; 1; .
PfamPF00476; DNA_pol_A; 1; .
PfamPF01612; DNA_pol_A_exo1; 1; .
PRINTSPR00868; DNAPOLI; .
SMARTSM00474; 35EXOc; 1; .
SMARTSM00475; 53EXOc; 1; .
SMARTSM00278; HhH1; 1; .
SMARTSM00279; HhH2; 1; .
SMARTSM00482; POLAc; 1; .
SUPFAMSSF47807; 5_3_exo_C; 1; .
SUPFAMSSF53098; RNaseH_fold; 1; .
TIGRFAMsTIGR00593; Pola; 1; .
PROSITEPS00447; DNA_POLYMERASE_A; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server